Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacteriology > Lab 3 Streptococci, Staphylococci > Flashcards

Lab 3 Streptococci, Staphylococci Flashcards

1
Q

Staphylococci habitat and morphology

A

Habitat: skin, mm, food/plant, soil, water
Morphology: coccus, bunch of grapes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Staphylococci culture and pigment

A

Simple culture: nutrient agar and nutrient broth

Pigment: caroteinoid (gold-ish)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Give two selective cultures used for Staphylococci and what they detect

A
  1. Mannitol-salt agar (with pH indicator: phenol)
    Detects s. aureus (and micrococci) colour change!
  2. Baird-Parker agar (with Na-tellurite, glycine, pyruvic acid and egg)
    Can detect staph coagulase positive!
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Staphylococci catalase and oxidase

A

Catalase +

Oxidase -

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What EC enzymes do staphylococci produce?

A

Coagulase
Fibrinolysin
Hyaluronidase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What surface proteins and toxins do staphylococci have?

A

Surface proteins: protein-A:
can bind immunoglobulins and hide from immune system of host

Toxins: 
haemolysins, 
leucosidins (WBC damage),
enterotoxins
toxic shock syndrome toxins!
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Do staphylococci produce spores? What resistance do they have?

A

No spores

Good resistance

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Give pathogenicity of staphylococci (7)

A 
Local suppuration
Abcesses
Arthritis
Mastitis
Metritis
Dermatitis
Septicaemia
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Give some characteristics of coagulase POS species of staphylococci

A 
Many extracellular enzymes
Hemolysis
Mannitol fermentation
Toxin production
Facultative pathogenicity
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Give some characteristics of coagulase NEG species of staphylococci

A 
Few extracellular enzymes
No hemolysis or mannitol fermentation
No or little toxin production
No or subclinical pathogenicity
Generally saprophytes
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

S. aureus subsp. aureus

A

Staph
Biotypes A-E (different hosts)
MRSA: methicillin resistant s. aureus
Coag+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

S. aureus subsp. anaerobius

A

Staph
Morel-disease in sheep (lymphadenitis)
Coag+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

S. pseudointermedius

A

Staph
Dermatitis/otitis externa in Ca and Fe
Coag+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

S. intermedius

A

Staph
Ca, Eq, Av: mucous membranes
Saprophyte
Coag+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

S. epidermidis

A

Staph
Frequent wound infection in Ca and Eq
Coag-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

S. haemolyticus

A

Staph
Milk
Coag-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

S. hyicus

A

Staph
Some strains are coag pos!
Porcine exudative epidermitis (greasy pig disease)
Coag-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

S. gallinarum
S. equorum
S. felis

A

Staph
Dermatitis
Coag-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Micrococcus habitat and morphology

A

Habitat: environment, mm, food, skin
Morphology: cocci, clusters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Micrococcus biochemistry and pathogenicity

A

Biochem: decomposition of glucose is aerobic or missing
Pathogenicity: saprophyte
Catalase and oxidase pos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

M. luteus

A

Yellow colonies, grape clusters

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Streptococci habitat and morphology

A

Habitat: skin, mm, food, milk, digestive tract
Morphology: coccus, in chains (NOT CLUSTERS) diplococci
Some species have a capsule (hyaluronic acid or polysaccharide)

23
Q

Streptococci cultures

A

Fastidious: blood/serum agar (some need CO2)

Selective culture: Crystal Violet, Thallium-SO4, esculin, blood (Edwards)

24
Q

What type of haemolysis do you see in Streptococci compared to Staphylococci

A

Strep: alpha, beta and gamma hemolysis
Staph: only beta and gamma hemolysis

25
Q

What streptococci is detected with CAMP test

A

S. agalactiae (in presence of s. aureus)

26
Q

Streptococci catalase and oxidase

A

Catalase neg and oxidase neg

27
Q

Streptococci utilization of glycosides

A

Esculin, salicin

28
Q

Streptococci antigens

A

Complex
Lancefield A-W
Other type specific antigens
Capsule antigens (S. suis, S. pneumoniae)

29
Q

Streptococci resistance and pathogenicity

A 
Resistance: good
Local suppuration
Metritis, mastitis, arthritis
Septicaemia
Strangles (!)
Pneumonic diseases
30
Q

S. pyogenes:

A

Strep
capsule
toxin,
Hu: scarlet fever, rheumatic fever, glomerulonephritis

31
Q

S. agalactiae, S. dysgalactiae (subsp. equismilis), S. uberis

A

Strep
Ruminants
Mastitis
Subsp. equismilis: lymphadenitis, abortion in Eq

32
Q

S. equi

A

Strep
Subsp. equi: horse strangles
Subsps. zooepidemicus: suppuration, septicaemia, mastitis, abortion

33
Q 
S. suis
S. porcinus
S. canis
S. bovis
S. salivarius, sanguis, mutans
S. pneumoniae
A 
Strep
Suis: septicaemia, arthritis, meningitis
Porcinus: lymphadenitis, abcesses
Canis: metritis, neonatal septicaemia
Bovis: septicaemia (pigeon)
SSM: oral streptococci
Pneumoniae: calf, foal, human: pneumonia, meningitis
34
Q

Enterococcus culture and Lancefield category

A

Culture: 10-45 C, pH 9,6, Lancefield D

Forms chains

35
Q

Enterococcus species

A

E. faecalis (hemolysis)
E. faecium
E. avium
E. gallinarum

36
Q

Enterococcus habitat, pathogenicity

A

Gut, mainly saprophytes

Sometimes endocarditis, abscesses

37
Q

Lactococcus fermenters and Lancefield category

A

Fast lactose fermenters
Found in gut flora (Probiotic)
Lancefield N

38
Q

Lactococci species and subspecies

A

L. lactis subsp. lactis
L. lactis subsp. cremoris
L. plantarum (silage)

39
Q

Anaerobic cocci habitat and species

A 
Habitat: mucous membranes, mainly saprophytes1
Peptococcus
Peptostreptococcus
Peptoniphilus
Ruminococcus
Sarcina
40
Q

Lactobacillus habitat and morphology

A

Habitat: mm, gut, feed, plants
Morphology: long, thin (sometimes curved) rods

41
Q

Lactobacillus culture (temp and pH)

A

Microaerophilic
Temp. 30-40C (2-53)
pH 5.5-6.2

42
Q

Lactobacillus production and use

A

Produces lactate from lactose (pH 4)

Used in dairy industry (yogurt etc)

43
Q

Erysipelothrix habitat and morphology

A

Habitat: alimentary tract, mid, slime of fish, water
Morphology: small thin rods (s) filaments (r)

44
Q

Erysipelothrix culture

A

Nutrient agar
Nutrient broth: S: uniform, R: sediment
NaN3 + Crystal violet, neomycin

45
Q

Erysipelothrix catalase and oxidase

A

Catalase -

Oxidase -

46
Q

Erysipelothrix extracellular enzymes

A

Neuroaminidase

Hyaluronidase

47
Q

Erysipelothrix resistance

A

Good, halotolerance, soil

Alpha hemolysis

48
Q

Erysipelothrix pathogenicity

A 
Facultative pathogenic
Su: erysipelas
Ov: wound infection
Av: septicaemia
Hu: wound inf
49
Q

E. tonsillarum

A

Present in tonsils of Su

Endocarditis in dogs

50
Q

Listeria habitat and morphology

A

Habitat: soil, sewage, plants, silage, gut, food
Morph: thick rods, flagella (in room temp)

51
Q

Listeria culture

A

Simple: nutrient agar/broth
Wide temp range
pH 5.5-9.6
Selective: cattle serum - trypaflavin, nalidixic acid (U-tube)

52
Q

Listeria catalase and oxidase

A

Catalase +

Oxidase -

53
Q

Listeria virulence factors and antigens

A 
Listeriolysin (haemolysin) IC replication
Monocytosis factor (cell wall, lipoid)

Antigens: cell wall polysaccharide
flagellar protein
13 serotypes

54
Q

Listeria resistance and pathogenicity

A

Good: propagation in soil

Facultative pathogenic:
haemolysis, lipolysis, monocytosis
Sheep: encephalitis, abortion
Cattle: encephalitis, abortion
Rabbit: septicaemia, abortion
Humans: abortion, neonatal septicaemia, encephalitis
Birds: septicaemia

Bacteriology (13 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions