26- Molecular techniques review lecture

Question 1

Wild type fragments

Answer 1

restriction endonucleases can cleave MstII site- recognises sequence

0. 4kb
1. 6kb
2. 2kb
3. 9kb

Question 2

sicke cell fragments

Answer 2

(No MstII site to cleave)

0. 4kb
1. 8kb (0.6kb +1.2 Kb)
2. 9 kb

Question 3

sicke cell traits (SCT) fragments

Answer 3

heterozygous

Has both HbA and HbS genes on different alleles

0. 4kb
1. 6 kb
2. 2 kb
3. 9 kb
4. 8 kb

Question 4

probes

Answer 4

(radioactive or fluorescent) added and will bind to specific sequences

Question 5

probe 1

Answer 5

e.g. Probe 1

WT: will only have complementary DNA for 0.6kb section, so will only bind there and not 0.4, 1.2 or 0.9k.

SC: only bind top 1.8 segment

SCT: will bind to 1.8 and 0.6kb segment

Question 6

Answer 6

Normally when designing a primer you will include al the sequence you want to PCR

• can do primers that are complementary for outside the ORF