Rapid Diagnosing Infectious Disease Flashcards
What 4 tests can be done on samples
Antibody test
Antigen assay test
Molecular assay test(testing for genes)
Cultivation in agar test
What are the 4 types of cultivation of bacteria
Selective : certain conditions for certain species
general - non selective
Enrichment - selects for certain bacteria
Differential - selection between species
What does differential cultivation use
Chromogenic reactions with enzymes and substrates which turn bacteria a different colour
Why would urine be cloudy if infected
CFU present
What is latex agglutination test
testing for antigens present in sample with antibodies attached to latex beads
Why is latex agglutination an example of serology
Serology tests for specific antibodies/ antigen relationships
How is serotyping different to serology
Serotyping is identifying different subgroups of organisms eg of ecoli
What 3 things are subtypes dependant on
O antigen
H antigen
K antigen
What is the biochemical assay test
Using the unique bacterial metabolic activities to identify it on a slide with eg enzymes present
Give 4 advantages to using a molecular diagnostic technique (DNA)
Genome tracking is important for evolution
Genetic material can detect where it’s from
Dna is easily amplified
Molecular diagnosis is sensitive to CFU
Name 4 disadvantages of using dna molecular techniques
Technology is expensive
Some tests require bacteria to be isolated = long time
Some cross over in sample due to sensitivity for CFU
Standardisation tests needed in labs
Which 2 ways are molecular tests used to identify presence
Elisa or PCR with gel electrophoresis
Why is the PCR used in testing called real time
Because a binding dye or agent/ gel will illuminate as soon as product is growing in PCR
Name 3 types of agents used in RT PCR
Sybr green- binds to dna and luminate green
FRET probe
Dna probe
Out of the reporter and quencher , what is knocked off when dna grows in RT PCR
Reporter then quencher
