Tools for lab Flashcards

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1
Q

Culture

A

To grow a microbe

Sample or group of microbes

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2
Q

Medium

A

nutrients for the growth of microbes

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3
Q

Inoculum

A

a small sample of microbes

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4
Q

Inoculation

A

a sample microbe is placed in media with a tool

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5
Q

Clinical Specimens

A

from the bodily fluids, discharges, anatomical sites, diseased tissue

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6
Q

Incubated

A

placed in a temperature-controlled area

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7
Q

Cultured Media uses

A

Flask, Petri dishes, and tubes

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8
Q

Media inoculated with

A

Needle, loops, swabs, pipes

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9
Q

Media Classified by

A

Physical state, chemical composition, and function(purpose)

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10
Q

Agar

A
complex polysaccharide from red algae;
Solid at room temp
Melts at 100 C
holds moisture and nutrients
Microorganisms cannot digest
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11
Q

Physical states of media

A

Liquid(Nutrient broth)
Solid that can be liquid( media has levels of agar)
Solid that cannot be converted to liquid
Semi-solid (media has low levels of agar)

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12
Q

Chemical Composition of Media:

Defined/ Synthetic

A

We know all the components of media chemical

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13
Q

Chemical Composition of Media:

Complex

A

One component of media is not precisely defined

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14
Q

Media Purpose/Function:

General-purpose media

A

Allow the growth of a broad spectrum of microbes

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15
Q

Media Purpose :

Enriched media

A

grow microbes that need special growth factors

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16
Q

Fastidious

A

microbes that must have special growth factors and complex nutrients

17
Q

Selective media

A

can inhibit the growth of certain microbes but not inhibit the growth of other microbes

18
Q

Differential media ;

changes you might see

A

show the visible differences in how microorganisms grow.
variation in colony size or color
media might change color
gas bubbles

19
Q

Reducing medium

A

cystine will absorb O2 or slow the penetration of 02 in a medium.
Use when culture anaerobic bacteria

20
Q

Carbohydrate fermentation media

A

has sugars that are fermented and converted to acids and a pH indicator to show this reaction

21
Q

Transport media

A

used to maintain specimens that have to be held for a period of time before sent to a lab

22
Q

Isolation

A

the single microbe is separated from other cells on a nutrient surface and form a colony

23
Q

Colony

A

a mound of the same species of microbe from isolation

24
Q

Streak plate method

A

a small drop of culture is spread over media with a loop in a pattern to thin out culture
this allows a single cell to grow into a colony

25
Q

Loop dilution/ pour plate

A

the sample is inoculated multiple times in a cooled agar tube to dilute the number of cells in each tube.
end result is to have a volume of cells decreased so cells may have room to grow in colonies

26
Q

Spread plate technique

A

the diluted sample is piped onto media and spread evenly

this will push cells into separate areas so they can form colonies

27
Q

How are microbes identified

A
microscope
metabolic features
their nutrient needs
enzyme presence 
genes