Tools for lab

Question 1

Culture

Answer 1

To grow a microbe

Sample or group of microbes

Question 2

Medium

Answer 2

nutrients for the growth of microbes

Question 3

Inoculum

Answer 3

a small sample of microbes

Question 4

Inoculation

Answer 4

a sample microbe is placed in media with a tool

Question 5

Clinical Specimens

Answer 5

from the bodily fluids, discharges, anatomical sites, diseased tissue

Question 6

Incubated

Answer 6

placed in a temperature-controlled area

Question 7

Cultured Media uses

Answer 7

Flask, Petri dishes, and tubes

Question 8

Media inoculated with

Answer 8

Needle, loops, swabs, pipes

Question 9

Media Classified by

Answer 9

Physical state, chemical composition, and function(purpose)

Question 10

Agar

Answer 10

complex polysaccharide from red algae;
Solid at room temp
Melts at 100 C
holds moisture and nutrients
Microorganisms cannot digest

Question 11

Physical states of media

Answer 11

Liquid(Nutrient broth)
Solid that can be liquid( media has levels of agar)
Solid that cannot be converted to liquid
Semi-solid (media has low levels of agar)

Question 12

Chemical Composition of Media:

Defined/ Synthetic

Answer 12

We know all the components of media chemical

Question 13

Chemical Composition of Media:

Complex

Answer 13

One component of media is not precisely defined

Question 14

Media Purpose/Function:

General-purpose media

Answer 14

Allow the growth of a broad spectrum of microbes

Question 15

Media Purpose :

Enriched media

Answer 15

grow microbes that need special growth factors

Question 16

Fastidious

Answer 16

microbes that must have special growth factors and complex nutrients

Question 17

Selective media

Answer 17

can inhibit the growth of certain microbes but not inhibit the growth of other microbes

Question 18

Differential media ;

changes you might see

Answer 18

show the visible differences in how microorganisms grow.
variation in colony size or color
media might change color
gas bubbles

Question 19

Reducing medium

Answer 19

cystine will absorb O2 or slow the penetration of 02 in a medium.
Use when culture anaerobic bacteria

Question 20

Carbohydrate fermentation media

Answer 20

has sugars that are fermented and converted to acids and a pH indicator to show this reaction

Question 21

Transport media

Answer 21

used to maintain specimens that have to be held for a period of time before sent to a lab

Question 22

Isolation

Answer 22

the single microbe is separated from other cells on a nutrient surface and form a colony

Question 23

Colony

Answer 23

a mound of the same species of microbe from isolation

Question 24

Streak plate method

Answer 24

a small drop of culture is spread over media with a loop in a pattern to thin out culture
this allows a single cell to grow into a colony

Question 25

Loop dilution/ pour plate

Answer 25

the sample is inoculated multiple times in a cooled agar tube to dilute the number of cells in each tube.
end result is to have a volume of cells decreased so cells may have room to grow in colonies

Question 26

Spread plate technique

Answer 26

the diluted sample is piped onto media and spread evenly

this will push cells into separate areas so they can form colonies

Question 27

How are microbes identified

Answer 27

microscope
metabolic features
their nutrient needs
enzyme presence 
genes