ABOH and Se

Question 1

Most important blood group system

Answer 1

ABO

Question 2

What is the inheritance pattern for A B and O antigens

Answer 2

A and B are co-dominant over O (recessive)

Question 3

Which chromosome are ABO alleles on

Answer 3

Chromosome 19

Question 4

Which substrate are ABO antigen built on

Answer 4

H

Question 5

Precursor for H

Answer 5

Type 2 precursor chain

Question 6

Name for the H gene

Answer 6

FUT1 gene (built on type 2 precursor chain)

Question 7

Transferase that the H gene codes for

Answer 7

L-fucosyl transferace (L-fucose)

Question 8

Chromosome location for ABO vs H

Answer 8

ABO- chromosome 9
H- chromosome 19

Question 9

What type of gene is O

Answer 9

Amorph recessive

Question 10

What transferase does A code for

Answer 10

N-Acetylgalactosaminyltransferase
(N-Acetylgalactosamine sugar, also called N-Acetyl-D-galactosamine)

Question 11

What transferase does B code for

Answer 11

D Galactosyl transferase (D-galactose sugar)

Question 12

If you inherit both A and B, which one will have more antigens? (AB type)

Answer 12

B is more competitive and there will be more B than A antigens

Question 13

At what age do ABO antigens develop

Answer 13

5-6 weeks gestation

Question 14

At what age do ABO antigens fully develop

Answer 14

2-4 years

Question 15

ABO frequencies for Whites

Answer 15

O 45%
A 40%
B 11%
AB 4%

Question 16

ABO frequencies for Blacks

Answer 16

O 50%
A 26%
B 20%
AB 4%

Question 17

ABO frequencies for Hispanics

Answer 17

O 56%
A 31%
B 10%
AB 3%

Question 18

ABO frequencies for Asians

Answer 18

O 40%
A 28%
B 25%
AB 7%

Question 19

When do ABO antibodies start to develop

Answer 19

As early as 3 months after birth, usually 4-6 months
They are naturally occurring

Question 20

What antibody class are ABO antibodies

Answer 20

Predominantly IgM for group A and B people (IgM is the general answer)
Predominantly IgG for group O people

Question 21

What type would ABO antibodies detected at birth be?

Answer 21

IgG because IgM cannot cross placenta. Any antibodies seen are moms, so moms IgG ABO antibodies are the ones observed on baby

Question 22

Antibodies present in group O people

Answer 22

Anti-A, anti-B, and anti-A,B

Question 23

What type are reagent cells A1 and B

Answer 23

A1 are type A1 Rh negative
B are type B Rh negative

Question 24

Why is a saline control needed when typing AB people

Answer 24

To rule out possibility of panreactivity or autoagglutination

Question 25

Precursor substance for Se

Answer 25

Precursor substance 1 or type 1 chain

Question 26

Name for Se gene

Answer 26

FUT2

Question 27

Precursor substances

Answer 27

On RBC (H) type 2 On secretions (Se) type 1

Question 28

FUT genes

Answer 28

FUT1 H FUT2 Se

Question 29

What transferase does Se produce

Answer 29

L-fucosyl transferase (same as H, which is why there is a H-like antigen in secretions)

Question 30

Percentage of population that are secretors

Answer 30

80%

Question 31

Bombay inherited alleles

Answer 31

hh/sese

Question 32

Parabombay inherited alleles

Answer 32

hh/Sese

Question 33

Difference between Bombay and Parabombay

Answer 33

Bombay don’t have H at all, parabombay have Se gene so they have H and can have A/B in their secretions, just not on rbc

Question 34

What would a Bombays ABO and screen look like

Answer 34

aBO would look normal like a type O. Screen would be reactive with all cells at all phases except for the autocontrol

Question 35

How to identify a bombay

Answer 35

Test with anti-H should be negative (ulex europaeus). Need RESt adsorptions to remove anti-H (cold)

Question 36

A1 vs A2

Answer 36

A1 people have A and A1 antigens A2 people only have A 80% of A people are A1

Question 37

How to test for A1 vs A2

Answer 37

A1 will react with anti-A1 lectin A2 will not A2 people can make anti-A1

Question 38

Which A subgroups can make anti-A1

Answer 38

A2, A3, Ax are the main ones (not always and not naturally occurring for A2) Ax and Ael usually make anti-A1 due to very weak A expression

Question 39

Which A subgroups would show MF reactions when typing

Answer 39

A3 and Aend

Question 40

Which A subgroup will react with anti-A1

Answer 40

A1 only

Question 41

Name for A1 lectin

Answer 41

Dolichos Bifloris

Question 42

Name for H lectin

Answer 42

Ulex europaeus

Question 43

Which B subgroup shows MF

Answer 43

B3

Question 44

Weakest subgroups of A and B

Answer 44

Ael and Bel Type as O, need adsorption elution

Question 45

Greatest to leas amount of H on ABOs

Answer 45

O>A2>B>A2B>A1>A1B

Question 46

Name for B lectin

Answer 46

Bandeiraea simplicifolia