Ackerman Lectures 3 and 4 Flashcards Preview

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Flashcards in Ackerman Lectures 3 and 4 Deck (27):
1

lactate dehydrogenase

reduces pyruvate to lactate, uses NADH as cofactor, replenishes NAD+ (req for glycolysis) (anaerobic cells only)

2

aerobic pyruvate breakdown

oxid decarboxy to CO2 and acetyl coA cat by PDC; 2C acetyl coA then oxid to 2mol CO2 in TCA cycle

3

mito matrix contents

PDC, CAC enz's, beta oxid enz's, AA oxid enz's, DNA, ribosomes, ATP, ADP, Pi, Mg, Ca, K; soluble metab intermeds

4

outer memb

freely permeable to small molecs and ions

5

inner memb

imperm to most small molecs and ions, including H+; has resp e- carriers, ADP-ATP translocase, ATP synthase, other transporters

6

PDH

oxidatively decarboxylates pyruvate to CO2 and acetyl CoA, yields 1 mol NADH per mol pyruvate; E1, E2, E3

7

3 cofactors bd perm to PDH

TPP, lipoic acid, FAD

8

2 cofactors that enter/exit PDH w/each turnover

CoA-SH, NAD+

9

E1

pyruvate DH (TPP)

10

E2

dihydrolipoyl transacetylase (lipoic acid)

11

E3

dihydrolipoyl DH (FAD)

12

PDH products

CO2, then acetyl-coA, then NADH

13

citrate synthase

acetyl-CoA + OAA --> citrate (H2O to CoA-SH); -7.7

14

aconitase

citrate --> cis-aconitate --> isocitrate (lost 2 H2O); +3.2

15

isocitrate dehydrogenase

isocitrate --> a-KG; NAD to NADH + H+; rate limiting step; -2.0

16

a-ketoglutarate dehydrogenase complex

a-KG --> succinyl-CoA; NAD+ to NADH and CoA-SH; -8.0

17

succinyl-CoA synthetase

succinyl-CoA-->succinate; GDP + Pi-->GTP; CoA-SH; NTP synth; -0.7

18

succinate dehydrogenase

succinate-->fumarate (FAD to FADH2); 0

19

fumarase

fumarate-->L-malate (lose OH-); -0.9

20

malate dehydrogenase

L-malate-->OAA (NAD+ to NADH + H+); very unfavorable; +7.1; OAA can't leave, but malate can

21

TCA inhibition

high E charge; ATP, NADH, acetyl-CoA, FAs

22

TCA stimulators

Ca, AMP, CoA, NAD+, ADP

23

amphibolic

both catabolic and anabolic; produces E and provides metabolites for biosynth of new material

24

anapleurotic

intermeds constantly replenished by metabolite input from other paths

25

catabolic action

NADH, FADH2, GTP (degrad rxns yield high-E metabolites)

26

anabolic action

amino acids (aKG, OAA), FA and cholesterol (acetyl CoA via citrate), porphyrins (succ-CoA), glucose (OAA via malate)

27

carbon sources/feeding steps

AAs (aKG, OAA, succ-CoA, fumarate), odd-chain FA (succ-CoA), pyruvate + CO2 (OAA)