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aerobic g+ bacilli Flashcards

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1
Q

normal flora of human nasopharynx

A

c.pseudodiphtheriticum

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2
Q

responsible for the s/s of anthrax

A

protein exotoxins

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3
Q

acute, contagious disease characterized by systemic toxin and false membrane lining of mucus membrne of throat which leads to respiratory obstruction

A

diphtheria

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4
Q

growth factor of coryne

A

8 amino acids

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5
Q

biggest pathogen

A

b.anthracis

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6
Q

b.anthracis is part of normal flora, T/F?
motile or nonmotile?
__% NaCl is needed

A

F
nonmotile
7%

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7
Q

appearance of b.anthracis on culture (3)

A

medusa head colonies
beaten egg white appearance
non hemolytic

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8
Q

b.anthracis grows in?
produces?
ferments?

A

low pH (

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9
Q

virulence factor of b.anthracis (2)

A

d-glutamic capsule (antiphagocytic)

protein exotoxins

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10
Q

combination of these protein exotoxins may lead to death

A

protective antigen

lethal factor

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11
Q

combination of these protein exotoxins may lead to edema

A

edema factor

protective antigen

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12
Q

other name of pulmonary anthrax

A

woolsorter’s disease

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13
Q

3 colonial type of ctba, bap

A

mitis
intermedius
gravis

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14
Q

growth factor of b.anthracis

A

thiamine (B1)

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15
Q

MOT of b.anthracis (2)

A

inhalation

traumatic introduction

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16
Q

3 types pf anthrax

A

cutaneous
pulmonary
gastrointestinal

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17
Q

appearance of cutaneous anthrax

A

black eschar appearance

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18
Q

closely related to nocardia and mycobacteria

A

corynebacterium

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19
Q

fried rice bacillus

A

bacillus cereus

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20
Q

most commonly encountered bacillus in oppurtunistic inections

A

b.cereus

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21
Q

weapon for mass destruction

A

b.anthracis

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22
Q

virulence factor of b.cereus

A

enterotoxins (heat stable & heat labile)

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23
Q

which type is assocuated with ingestion of contaminated meat, vegetables, poultry?
incubation period?
symptoms?
virulence factor present?

A

diarrheal type
long incubation (8-16 hrs)
diarrhea
(+) heat labile enterotoxin

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24
Q

which type is associated with ingestion of improper stored fried rice/reheated rice?
incubation period?
symptoms?
virulence factor present?

A

emetic type
short incubation (1-6 hrs)
vomiting
(+) heat stable enterotoxin

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25
common laboratory contaminant
bacillus subtilis
26
result when virulent strains repeatedly subcultured in vitro
avirulent
27
sporeforming, rod shaped organisms
bacillus
28
appearance of b.anthracis in microscope
bamboo fishing rod appearance | unstained central spore
29
susceptible to penicillin? | appearance?
b.anthracis | string of pearl appearance
30
virulence factor of. c.diphtheriae
diphtheriae toxin
31
source of bacitacin antibiotic
b.subtilis
32
appearance of b.subtilis in culture? | ferments? (3)
ground glass appearance | mannitol, xylose, arabinose
33
specimens used for lab diagnosis of b.subtilis (3)
malignant pustule sputum blood
34
specimens used for coryne
throat, nasopharyngeal swabs
35
used to observe the capsule of b.anthracis in blood or csf?
india ink
36
coryne are glucose and maltose fermenters except (2)
c. urealyticum | c. psedodiphtheriticum
37
2 types of food poisoning caused by b.cereus
diarrheal | emetic
38
PLET means
polymyxin lysozyme EDTA thallous acetate
39
enrichment and selective tchnique
heat/alcohol shock technique before plating on media
40
medium used for isolation of bacillus spp from contamintaed specimen
PEA
41
flagella of bacilli
peritrichous
42
give the characteristics of coryne (3)
nonmotile non sporeforming non encapsulated pleomorphic rods
43
animal, plant and human pathogens
corynebacterium
44
apperance of b.cereus in BAP
frosted glass colonies beta-hemolytsis feathery
45
c.diphtheriae is +/- to urease?
(-)
46
medium which induces the capsule formation of b.anthracis
bicarbonate agar
47
appearance of b.anthracis in gelatin medium? | (+) result?
inverted pine tree | liquefaction of b.anthracis
48
agar used with an appearance of opaque zone (lecithinase formation) of b.anthracis
egg yolk agar
49
most frequently isolated coryne
c.urealyticum
50
hay bacillus
b.subtilis
51
most clinically significant soecies of bacillus
bacillus cereus grp
52
biochemical test: bacillus catalase +/-? ferments? hydrolyzes?
+ glucose starch
53
other name of c.diphtheriae
kleb loeffler's bacillus
54
appearance fo coryne in microscope
club shape bacteria (slightly curved, rod, silightly wider ends, unparallel sides
55
means red disease
rhusiopathiae
56
diphtheria can cause (2) | appearance?
tissue necrosis, exudates formation | gray to white pseudomembranous formation
57
c.diphtheriae can be killed by (2)
heat | disinfectants
58
cell wall of coryne contains ___ which is composed of? (2)
mesodiaminopimelic acid/ m-DAP | diamino acid, short chain mycolic acid
59
diphtheria toxin are nontoxic, unless exposed to? | process is called?
trypsin | trypsinazation
60
characterized by slow healing ulcers and membrane formation
cutaneous form
61
c.diphtheriae grp (3)
c. diphtheriae c. ulcerans c. pseudotuberculosis
62
means red skin, thread
erysipelothrix
63
cells are arranged in chinese letters, palisades in pairs
coryne
64
culture media used for coryne
CTBA tinsdale agar loeffler serum agar
65
ctba and tellurite media should be incubated for how long?
48 hrs in ambient air
66
used to enhance recovery form clinical soecimens (placental tissues) what temperature?
cold enrichment technique | 4 C
67
addition of ___ inhibits toxin production of coryne very quickly
iron
68
swab used in collecting the specimen for coryne
calcium alginate swab
69
prefrered medium for coryne
CTBA
70
disease affecting farm animals by feeding on plants contaminated with the spores
anthrax
71
only catalase negative that produces h2s
erysipelothrix rhusiopathiae
72
test which detects anthrax antigen rgt used? to observe?
ascoli test rabbit antiserum precipitin formation
73
culture media used for bacilli (5)
``` BAP CAP PEA PLET nutrient broth ```
74
respiratory form of diphtheria can cause (2)
pharyngitis | death
75
skin test for bacillus spp
ascoli test
76
urinary pathogen
c.urealyticum
78
skin test for c.diphtheriae
schick's test
79
tinsdale agar is composed of (5) | (+) result
``` sheep's blood bovine serum cystine potassium tellurite sodium thiosulfate (+) black colonies with brown halo ```
80
forms of diphtheria (2)
respiratory form | cutaneous form
81
agar used for oberving morphology and metachromatic granules? (+) result? metachromatic granules w/in the cells?
loeffler's serum agar poached egg colonies babes-ernst granule
82
serious infection primrly of neonates, preggy and immunocomprmsd hosts
listeriosis
83
to observe urease production of c.urealyticum
christensen urea slant
85
immunodiffusion test? | (+) result?
elek plate test | 4-5 mm fine precipitin lines at 45 degree angle to the streaks
86
it can grow in high salt concn (10% NaCl)
listeria monocytogenes
87
medium used for selection and isolation of bacillus spp from contamintaed specimen
PLET
88
appearance of erysipelothrix rhusiopathiae in gelatin stab culture
pipe cleaner/test tube brush pattern
89
in vitro test? | in vivo test?
elek plate test | guinea pig lethal test
90
hoffman's bacillus
c.pseudodiphtheriticum
91
c.ulcerans (+) result?
gelatin formation
93
virulence factor of listeria monocytogenes
listeriolysin O
94
rapid urease producer
c.urealyticum
95
culture media used for listeria monocytogenes (2)
mcbride agar | nalidixic acid medium
96
listeria monocytogenes (+) result in CAMP
block type hemolysis
97
CTBA means
cystine tellurite blood agar
98
specimen for testing b.cereus
suspected food (>= 10 to the fifth cells/g)
99
bacillus in microscope
boxcar-shaped | unstained spores are clear
100
(+) result for motility test in listeria monocytogenes
tumbling motility | umbrella-shape/inverted xmas tree
101
immunocompromised and pregnant women shoul avoid eating?
soft cheese
102
b.cereus ferments?
salicin
103
c.pseudodiphtheriticum (+) result
nitrate production
104
common cause of diphtheroid prosthetic valve endocarditis
c.jeikeium
111
appearance of listeria monocytogenes in BAP
translucent grayish-blue colonies
119
coryne which produces dermonecrotic toxin
c.pseudotuberculosis
120
bacillus cereus grp (4)
b. cereus c. anthracis b. thuringiensis b. mycoides
121
(+) h2s production? | what medium?
erysipelothrix rhusiopathiae | triple sugar iron medium
122
c.diphtheriae on gram stain
highly pleomorphic g+ short or slightly curved rods with rounded ends

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