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Microbiology > aerobic g+ bacilli > Flashcards

aerobic g+ bacilli Flashcards

1
Q

normal flora of human nasopharynx

A

c.pseudodiphtheriticum

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2
Q

responsible for the s/s of anthrax

A

protein exotoxins

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3
Q

acute, contagious disease characterized by systemic toxin and false membrane lining of mucus membrne of throat which leads to respiratory obstruction

A

diphtheria

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4
Q

growth factor of coryne

A

8 amino acids

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5
Q

biggest pathogen

A

b.anthracis

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6
Q

b.anthracis is part of normal flora, T/F?
motile or nonmotile?
__% NaCl is needed

A

F
nonmotile
7%

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7
Q

appearance of b.anthracis on culture (3)

A

medusa head colonies
beaten egg white appearance
non hemolytic

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8
Q

b.anthracis grows in?
produces?
ferments?

A

low pH (

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9
Q

virulence factor of b.anthracis (2)

A

d-glutamic capsule (antiphagocytic)

protein exotoxins

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10
Q

combination of these protein exotoxins may lead to death

A

protective antigen

lethal factor

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11
Q

combination of these protein exotoxins may lead to edema

A

edema factor

protective antigen

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12
Q

other name of pulmonary anthrax

A

woolsorter’s disease

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13
Q

3 colonial type of ctba, bap

A

mitis
intermedius
gravis

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14
Q

growth factor of b.anthracis

A

thiamine (B1)

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15
Q

MOT of b.anthracis (2)

A

inhalation

traumatic introduction

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16
Q

3 types pf anthrax

A

cutaneous
pulmonary
gastrointestinal

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17
Q

appearance of cutaneous anthrax

A

black eschar appearance

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18
Q

closely related to nocardia and mycobacteria

A

corynebacterium

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19
Q

fried rice bacillus

A

bacillus cereus

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20
Q

most commonly encountered bacillus in oppurtunistic inections

A

b.cereus

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21
Q

weapon for mass destruction

A

b.anthracis

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22
Q

virulence factor of b.cereus

A

enterotoxins (heat stable & heat labile)

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23
Q

which type is assocuated with ingestion of contaminated meat, vegetables, poultry?
incubation period?
symptoms?
virulence factor present?

A

diarrheal type
long incubation (8-16 hrs)
diarrhea
(+) heat labile enterotoxin

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24
Q

which type is associated with ingestion of improper stored fried rice/reheated rice?
incubation period?
symptoms?
virulence factor present?

A

emetic type
short incubation (1-6 hrs)
vomiting
(+) heat stable enterotoxin

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25
Q

common laboratory contaminant

A

bacillus subtilis

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26
Q

result when virulent strains repeatedly subcultured in vitro

A

avirulent

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27
Q

sporeforming, rod shaped organisms

A

bacillus

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28
Q

appearance of b.anthracis in microscope

A

bamboo fishing rod appearance

unstained central spore

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29
Q

susceptible to penicillin?

appearance?

A

b.anthracis

string of pearl appearance

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30
Q

virulence factor of. c.diphtheriae

A

diphtheriae toxin

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31
Q

source of bacitacin antibiotic

A

b.subtilis

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32
Q

appearance of b.subtilis in culture?

ferments? (3)

A

ground glass appearance

mannitol, xylose, arabinose

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33
Q

specimens used for lab diagnosis of b.subtilis (3)

A

malignant pustule
sputum
blood

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34
Q

specimens used for coryne

A

throat, nasopharyngeal swabs

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35
Q

used to observe the capsule of b.anthracis in blood or csf?

A

india ink

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36
Q

coryne are glucose and maltose fermenters except (2)

A

c. urealyticum

c. psedodiphtheriticum

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37
Q

2 types of food poisoning caused by b.cereus

A

diarrheal

emetic

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38
Q

PLET means

A

polymyxin
lysozyme
EDTA
thallous acetate

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39
Q

enrichment and selective tchnique

A

heat/alcohol shock technique before plating on media

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40
Q

medium used for isolation of bacillus spp from contamintaed specimen

A

PEA

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41
Q

flagella of bacilli

A

peritrichous

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42
Q

give the characteristics of coryne (3)

A

nonmotile
non sporeforming
non encapsulated
pleomorphic rods

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43
Q

animal, plant and human pathogens

A

corynebacterium

44
Q

apperance of b.cereus in BAP

A

frosted glass colonies
beta-hemolytsis
feathery

45
Q

c.diphtheriae is +/- to urease?

A

(-)

46
Q

medium which induces the capsule formation of b.anthracis

A

bicarbonate agar

47
Q

appearance of b.anthracis in gelatin medium?

(+) result?

A

inverted pine tree

liquefaction of b.anthracis

48
Q

agar used with an appearance of opaque zone (lecithinase formation) of b.anthracis

A

egg yolk agar

49
Q

most frequently isolated coryne

A

c.urealyticum

50
Q

hay bacillus

A

b.subtilis

51
Q

most clinically significant soecies of bacillus

A

bacillus cereus grp

52
Q

biochemical test: bacillus
catalase +/-?
ferments?
hydrolyzes?

A

+
glucose
starch

53
Q

other name of c.diphtheriae

A

kleb loeffler’s bacillus

54
Q

appearance fo coryne in microscope

A

club shape bacteria (slightly curved, rod, silightly wider ends, unparallel sides

55
Q

means red disease

A

rhusiopathiae

56
Q

diphtheria can cause (2)

appearance?

A

tissue necrosis, exudates formation

gray to white pseudomembranous formation

57
Q

c.diphtheriae can be killed by (2)

A

heat

disinfectants

58
Q

cell wall of coryne contains ___ which is composed of? (2)

A

mesodiaminopimelic acid/ m-DAP

diamino acid, short chain mycolic acid

59
Q

diphtheria toxin are nontoxic, unless exposed to?

process is called?

A

trypsin

trypsinazation

60
Q

characterized by slow healing ulcers and membrane formation

A

cutaneous form

61
Q

c.diphtheriae grp (3)

A

c. diphtheriae
c. ulcerans
c. pseudotuberculosis

62
Q

means red skin, thread

A

erysipelothrix

63
Q

cells are arranged in chinese letters, palisades in pairs

A

coryne

64
Q

culture media used for coryne

A

CTBA
tinsdale agar
loeffler serum agar

65
Q

ctba and tellurite media should be incubated for how long?

A

48 hrs in ambient air

66
Q

used to enhance recovery form clinical soecimens (placental tissues)
what temperature?

A

cold enrichment technique

4 C

67
Q

addition of ___ inhibits toxin production of coryne very quickly

A

iron

68
Q

swab used in collecting the specimen for coryne

A

calcium alginate swab

69
Q

prefrered medium for coryne

A

CTBA

70
Q

disease affecting farm animals by feeding on plants contaminated with the spores

A

anthrax

71
Q

only catalase negative that produces h2s

A

erysipelothrix rhusiopathiae

72
Q

test which detects anthrax antigen
rgt used?
to observe?

A

ascoli test
rabbit antiserum
precipitin formation

73
Q

culture media used for bacilli (5)

A 
BAP
CAP
PEA
PLET 
nutrient broth
74
Q

respiratory form of diphtheria can cause (2)

A

pharyngitis

death

75
Q

skin test for bacillus spp

A

ascoli test

76
Q

urinary pathogen

A

c.urealyticum

78
Q

skin test for c.diphtheriae

A

schick’s test

79
Q

tinsdale agar is composed of (5)

(+) result

A 
sheep's blood
bovine serum
cystine
potassium tellurite
sodium thiosulfate
(+) black colonies with brown halo
80
Q

forms of diphtheria (2)

A

respiratory form

cutaneous form

81
Q

agar used for oberving morphology and metachromatic granules?
(+) result?
metachromatic granules w/in the cells?

A

loeffler’s serum agar
poached egg colonies
babes-ernst granule

82
Q

serious infection primrly of neonates, preggy and immunocomprmsd hosts

A

listeriosis

83
Q

to observe urease production of c.urealyticum

A

christensen urea slant

85
Q

immunodiffusion test?

(+) result?

A

elek plate test

4-5 mm fine precipitin lines at 45 degree angle to the streaks

86
Q

it can grow in high salt concn (10% NaCl)

A

listeria monocytogenes

87
Q

medium used for selection and isolation of bacillus spp from contamintaed specimen

A

PLET

88
Q

appearance of erysipelothrix rhusiopathiae in gelatin stab culture

A

pipe cleaner/test tube brush pattern

89
Q

in vitro test?

in vivo test?

A

elek plate test

guinea pig lethal test

90
Q

hoffman’s bacillus

A

c.pseudodiphtheriticum

91
Q

c.ulcerans (+) result?

A

gelatin formation

93
Q

virulence factor of listeria monocytogenes

A

listeriolysin O

94
Q

rapid urease producer

A

c.urealyticum

95
Q

culture media used for listeria monocytogenes (2)

A

mcbride agar

nalidixic acid medium

96
Q

listeria monocytogenes (+) result in CAMP

A

block type hemolysis

97
Q

CTBA means

A

cystine tellurite blood agar

98
Q

specimen for testing b.cereus

A

suspected food (>= 10 to the fifth cells/g)

99
Q

bacillus in microscope

A

boxcar-shaped

unstained spores are clear

100
Q

(+) result for motility test in listeria monocytogenes

A

tumbling motility

umbrella-shape/inverted xmas tree

101
Q

immunocompromised and pregnant women shoul avoid eating?

A

soft cheese

102
Q

b.cereus ferments?

A

salicin

103
Q

c.pseudodiphtheriticum (+) result

A

nitrate production

104
Q

common cause of diphtheroid prosthetic valve endocarditis

A

c.jeikeium

111
Q

appearance of listeria monocytogenes in BAP

A

translucent grayish-blue colonies

119
Q

coryne which produces dermonecrotic toxin

A

c.pseudotuberculosis

120
Q

bacillus cereus grp (4)

A

b. cereus
c. anthracis
b. thuringiensis
b. mycoides

121
Q

(+) h2s production?

what medium?

A

erysipelothrix rhusiopathiae

triple sugar iron medium

122
Q

c.diphtheriae on gram stain

A

highly pleomorphic g+ short or slightly curved rods with rounded ends

Microbiology (6 decks)

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