staph & strep (lab)

Question 1

most commonly isolated viridans strep

Answer 1

s.mutans

Question 2

test which detects unbound coagulase?
(+) result within?
screening?
confirmation?

Answer 2

tube method
clot formation within 4 hrs
1-4 hrs
20 hrs

Question 3

culture: BAP staph

Answer 3

buttery looking/ creamy,white or light gold

Question 4

staph
2 coagulase (-) species

Answer 4

s. epidermidis

s. saprophyticus

Question 5

bacitacin test or?
units?
to differentiate?
which is susceptible/resistant?

Answer 5

taxo A disk tes
0.04 units
micrococci (s; >10mm zone of inhibition) & staph (r)

Question 6

differentiates patho and nonpatho staph?
(+) result? in what organism?
agar used?
composed of?

Answer 6

mannitol fermentation test
yellow; s.aureus
MSA
1%mannitol and 7.5% NaCl

Question 7

most common isolated enterococci

Answer 7

e.faecalis

Question 8

test which detects dnase activity of s.aureus?
medium and rgt used?
(+) result?

Answer 8

DNAse test
DNA medium; methyl green dye
clearing of dye

Question 9

rgt for coagulase test

Answer 9

edta plasma

Question 10

staph
normal flora of skin?
cause what diseases? (2)

Answer 10

s.epidermidis

endocarditis, prostethic 💟 valve

Question 11

test for identification of s. saprophyticus?

zone of inhibition?

Answer 11

5 ug novobiocin: resistant

6-12 mm

Question 13

grp which is only susceptible to bacitracin

Answer 13

A

Question 14

s.epidermidis is ___ to novobiocin?

zone of inhibition?

Answer 14

susceptible

16-27mm

Question 15

coagulase (-) staph by tube method

Answer 15

s.lugdunensis

Question 16

characteristics of strep (5)

Answer 16

catalase (-)
fastidious
pairs, chains
facultative anaerobe
capnophilic

Question 17

alpha hemolytic strep

Answer 17

s.pneumoniae

Question 18

appearance in microscope (staph)

Answer 18

spherical in clusters

Question 19

beta hemolytic strep (4)

Answer 19

s. pyogenes
s. agalactiae
s. dysagalactiae, s.equi

Question 20

SXT means

Answer 20

sulfamethoxazole trimethoprim

Question 21

diseases caused by s.pnuemoniae

Answer 21

meningitis
lobar pneumonia
otitis media

Question 22

staph
cause of UTI in women?
urine culture?

Answer 22

s. saprophyticus

10,000 CFU/mL

Question 23

lab test results for viridans strep

Answer 23

bile insoluble

optochin resistant

Question 24

test for identification of agalactiae?
rgt?
(+) result?

Answer 24

CAMP test
beta-lysin disk
bowtie appearance

Question 25

CAMP means

Answer 25

christie
atkinson
munch
petersen

Question 26

rgt for hippurate hydrolysis test? | (+) result?

Answer 26

Na hippurate + ninhydrin | purple

Question 27

rgt for catalase test

Answer 27

3% h2o2

Question 28

most virulent staph

Answer 28

s.aureus

Question 28

presumptive test for s.pneumoniae? rgt? result?

Answer 28

optochin susceptibility/p disk test ethyhydroxycuprein hcl >= 14 mm

Question 29

enterococcus (3)

Answer 29

e. faecalis e. faecium e. durans

Question 32

(+) result for neufeld quellung

Answer 32

swelling of capsule

Question 33

tests which differentiate grp D and grp E enterococci? | tests which E is (-)

Answer 33

bile esculin, 6.5% NaCl, PYR | nacl, pyr

Question 34

gro C,F,G is ____ to bacitracin

Answer 34

resistant

Question 35

grp which is only susceptible to SXT

Answer 35

C

Question 36

rgt used for modified oxidase test? | (+) result?

Answer 36

1% tetramethyl-p-phenylenediamene dihydrochloride | purple

Question 36

test for identification of s.pyogenes? | result?

Answer 36

bacitracin | susceptible

Question 37

test which detects cell bound coagulase? | (+) result? within?

Answer 37

slide method | clot formation within 30 sec

Question 37

normal flora of URT, GIT, GUT

Answer 37

viridans strep

Question 38

confirmatory test for s.pneumoniae? rgt? incubate for how long? with tempt (+) result?

Answer 38

bile solubility Na desoxycholate 3 hrs at 35 C clear

Question 38

non enterococcus

Answer 38

s. bovis | s. equinis

Question 40

more specific test than bacitracin for pyogenes? rgt? (+) result?

Answer 40

L-pyrrolidonyl-alpha-naphthylamide test 0.01% p-dimethyl-aminocinnamaldehyde cherry red

Question 41

known as nutritionally variant strep? appearance in culture? growth factors?

Answer 41

abiotrophia and granulicatella satellites thiol compounds (cystein,pyrodoxal,vitb6)

Question 42

not classified under lancefield? | species?

Answer 42

viridans strep | s.mitis,mutans,sanguis

Question 43

give 5 charcteristics of staph

Answer 43

``` catalase-producing bacteria facultative anaerobe non motile non spore forming glucose fermenters ```

Question 43

gamma hemolytic strep

Answer 43

e. faecalis | e. facium