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entero-gram negative Flashcards

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1
Q

serological test identifies grp A strep through

A

latex agglutination/nucleic acid probe

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2
Q

rgt for catalase test

rxn to enterecocci

A

3% hydrogen peroxide

(-) no bubble formation

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3
Q

LAP means

A

Leucine aminopeptidase test

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4
Q

antigen detection

A

serological test

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5
Q

drug of choice for penicillin-resistant n.gonorrhea

A

ceftriaxone

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6
Q

differentiates aerococcus and leuonostoc to other g+ cocci

A

lap test

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7
Q

appearance of moraxella catarrhalis in culture

A

hockey puck appearance

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8
Q

young colonies of s.pneumoniae

A

dome-shaped
glistening
wet
mucoid

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9
Q

uses antibody bound to a particle to enhance visibility of agglutination rxn between Ag and Ab

A

co-agglutination test

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10
Q

modt common isolate inchildren with recurrent otitis media

A

s. pneumoniae

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11
Q

test which detects acetoin production from glucose

A

voges-proskauer test

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15
Q

virulence factor of s. pnuemoniae

A

capsular polysaccharide

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17
Q

present in martin lewis medium

A

all in modified TMA except nystatin, which is replaved by anisomycin: yeast

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18
Q

(+) for voges proskauer test

end product ?

A

anginosus grp

acetoin (red/pink color)

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19
Q

appear as satellites around an organism that produces pyridoxal

A

abiotrophia and granulicatella

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20
Q

s. pnemoniae in microscope

A

gram-positive cocci in pairs, oval or lancet shape, faculatatively anaerobe

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21
Q

(-) to LAP test

A

aerococci

leuconostoc

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22
Q

specimens used in lab diagnosis for s. pneumonie

A

sputum
blood
csf

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23
Q

growth factors of abiotrophia and granulicatella

A

thiol compounds (cystein,vit b6,pyridoxal)

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24
Q

most commonly isolated member of genus moraxella

A

moraxella catarrhalis

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25
Q

old colonies of s.pneumoniae

A

coin w/ raised rim

dimple/donut-shaped

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26
Q

gram stain: s. pneumoniae

A

direct smearsof csf reveal wbcsand gram+ cocci in pairs

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28
Q

3 differential tests for s. pneumoniae

A

optochin susceptibility/p disk test
bile solubility
neufeld quellung rxn

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30
Q

(neufeld quellung rxn)

this is mixed with thesputum,csf,etc along with methylen blue

A

antipneumococcal serum

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31
opsonized particles are more easily phagocytosed dueto presence of ___ on plasma membrane
complement receptors
34
``` *s.pneumoniae presumptive test? rgt used? susceptibility medium? result? ```
optochin susceptibility/p disk test ethylhydroxycuprein hydrochloride (6 or 10 um) BAP greater or equal to 14 mm
36
differentiates pneumococcus from viridans strep
bile solubility
37
compliction of pneumonia and otitis meia
meningitis
38
(+) result of m.catarrhalis in butyrate esterase test
blue color
39
(+)result of neufeld quellung rxn
swellingof capsule
40
formerly known as nutritionally variant streptococci
abiotrophia | granulicatella
41
appearance of neisseria in culture
small, gray-white opaque,convex, glistening colonies
42
capsule of s.pneumoniae is
antigenic
43
abiotrophia and granulicatella are(3)
pyridoxal/vit b6-dependent thiol-dependent symbiotic streptococci
44
opsonization of the capsule of s.pneumoniae renders the organism
avirulent
45
3rd most common cause of otitis media and sinusitis
moraxella catarrhalis
46
give at least 7 characteristics of neisseria
``` obligate aerobic non motile non hemolytic capnophiles (2-8%) sensitive to drying and extreme heat carbohydrate fermenters fastidious; enriched medium is used ```
47
bestmethod for culture andtransport of n.gonorrhea is
inoculate the agar immediately and place the medium in an increased CO2 such as coplin jar, JEMBEC system
48
culture media for meningitidis (3)
bap cap tma
49
neisseria observed in microscope | rod shaped?
gram-negative diplococci with coffee or kidney bean shaped | N. elongata, N. weaver
50
only member of family neisseriaceae which is (+) on DNAse test
m. catarrhalis
51
natural habitat of neisseria
mucuos membrane of respiratory & urogenital tract
52
n.menigitidis is sensitive to ___; this should not exceed ___
sps; 0.025%
53
beta lactamase test? rgt? (+) result?
cephalosporinase test cephalosporin disk deep pink
54
gram variable and pleomorphic in microscope
abiotrophia | granulicatella
55
*s.pneumoniae confirmatory test? rgt used? result?
bile solubility sodium desoxycholate(bile salt) (+) bile soluble, (-) turbid
57
biochemical identification of n.gonorrhea | (+) result?
coagglutination | agglutination
61
major pathogens of neisseria (2)
n. gonorrhea | n. meningitidis
62
presence of n.menigitidis in blood
meningococcemia
63
infection shows bloody, rust stinged sputum
lobar pneumonitis
64
other name for n. gonorrhea | only known host
gonococci | man
65
n. gonorrhea requires __ for growth it produces ___ which is important for pathogenesis virulence factor?(for adherence)
iron igA protease common pili
66
colonial typesof n.gonorrhea: virulent? avirulent?
t1 t2 | t3 t5
67
present in new york city medium
vancomycin colistin trimethoprim amphotericin B: yeast
68
in n.gonorrhea, these rare isolates are isolated from asymptomatic men
auxotypes
70
(n.meningitidis) clsi recommends what broth? and what agar microdilution? with?
MH broth with 2-5% laked horse blood | MHA with 5% sheep blood
72
AHU stains (3)
arginine hypoxanthine uracil
73
means "flow of seed" and "brothel"
gonorrhea
74
endotoxin produces hemorrhage in adrenals, which causes what syndrome
waterhouse-friderichsen syndrome
75
other name for conjuctivitis
opthalmia neonatorum
76
chief complaint oropharyngeal infection
pharyngitis
79
specimens used for n.gonorrhea
pus and secretions from cervix,urethra, throat,prostate
80
specimen collection for n.gonorrhea | then place inwhat transport medium? how long?
dacron or rayon swabs | amies medium for 6 hrs
81
screening test for n.gonorrhea
nucleic acid amplification
83
JEMBEC means
james e. martin biological environmental chamber
84
confirmation of n.gonorrhea is based on (2)
growth and biochemical characteristics
85
uses monoclonal bodies that recognizes epitope; | also confirms morphologic appearance
fluorescent antibody test/FAT
86
antimicrobial agents present in modified TMA, suppressed organism
all in TMA | trimethoprim plate: swarming of proteus
87
pyridoxal producers (4)
e.coli kliebsiella spp enterobacter spp yeast
88
virulence factors of n.meningitidis (4)
common pili capsular polysaccharide endotoxin igA1
89
confirmatory test for n.gonorrhea
chemiluminescent nucleic acid probe
90
present in GC-LECT medium
all in NYC medium | lincomycin: g+
91
other bacteria with (+) growth on gonococcal media (3)
acinetobacter capnocytophaga k.denitrificans
92
standard method in identifying n.gonorrhea detects? medium? (+) result?
CHO utilization test acid production from glucose, maltose, lactose, fructose cystine trypticase agar yellow color at 35-37C
93
rgt used for oxidase test in neisseria | (+) result?
1% tetramethyl-p-phenylenediamine dihydrochloride | purple color within 10 sec
95
s. pnuemoniae is also known as
diplococcus/pneumococcus
99
culture media for n.gonorrhea | if swabs, what pattern?
bap & cap | z-pattern and cross streaked with loop
104
antimicrobial agents present in TMA (3); and their suppressed organism
vancomycin: g+ colistin: g- nystatin: yeast
106
staph and strep are motile, T/F
F, brownian movement
112
untreated gonococcal cerviitis may cause
fitz-hugh-curtis synrome
138
(+) to LAP test? | end product?
viridans streptococci stretococcus enterococcus pediococcus B-naphthylamine (red color)

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