Ammonia

Question 1

Produced in the catabolism or deamination of _____and by bacterial metabolism in the lumen of the intestine

Answer 1

amino acids

Question 2

Ammonia

• Removed from the circulation and converted to urea in the____

Answer 2

liver

Question 3

Ammonia disease correlation

Answer 3

• Hepatic failure

• Severe liver disease

• Inherited deficiency of urea cycle enzymes

• Reye’s syndrome

Question 4

– causes swelling in the liver and brain

– often affects children and teenagers recovering from a viral infection, most commonly the flu or chickenpox

Answer 4

Reye’s syndrome

Question 5

Analytical method

• complicated by its low concentration, instability, and pervasive contamination

•__________________

Answer 5

GLUTAMATE DEHYDROGENASE ASSAY

Question 6

Specimen requirement

•_______ ammonia concentration increases rapidly following specimen collection because of in vitro amino acid deamination

•______ blood should be obtained without trauma and placed on ice immediately

•(2) tubes

• Samples should be centrifuged at _______ within 20 minutes of collection and the plasma or serum removed

• Hemolysis should be avoided

Answer 6

Whole blood

Venous

Heparin and EDTA

0°C to 4°C

Question 7

Interfering substances

INCREASED

Answer 7

• Ammonium salts

• Asparaginase

• Barbiturates

• diuretics

• Ethanol

• Hyperalimentation

• narcotic analgesics

Question 8

Interfering substances

DECREASED

Answer 8

• Diphenhydramine

• Lactobacillus acidophilus

• Lactulose

• Levodopa

• several antibiotics