Amniotic Fluid Flashcards

1
Q

Anti-hCG source

A

Rabbit

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2
Q

hCG peaks during

A

1st Trimester of Pregnancy

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3
Q

Priciple of Home-based HCG Pregnancy test Kit

A

Enzyme Immunoassay

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4
Q

Cut-off point for pregnancy test kit

A

25 mIU/mL

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5
Q

Ultimate source of amniotic fluid water and solutes, and is a product of fetal metabolism

A

Placenta

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6
Q

primary function of Amniotic fluid

A

Cushion for the fetus
Stabilizes temperature
Allows fetal movement
Proper kung development

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7
Q

Amniotic fluid volume is from

A

Fetal urine and lung fluid

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8
Q

Normal Amniotic fluid volume in 3rd trimester

A

800 - 1200 mL

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9
Q

During the 1st trimester 35mL of AF is derived from

A

Maternal circulation

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10
Q

Fetal urine is the major contributor to the AF volume AFTER the

A

1st Trimester

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11
Q

Placed on ICE DELIVERY, kept REFRIGERATED or FROZEN
filtration prevents loss of PHOSPHOLIPIDS

A

Test for FETAL LUNG MATURITY

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12
Q

Kept at ROOM TEMPERATURE or at 37C

A

Test for CYTOGENETICS STUDIES

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13
Q

Protected from Light

A

Test for HDN

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14
Q

Detects premature rupture of membranes, also used to diagnosed EARLY PREGNANCY

A

Fern Test

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15
Q

Colorless- Pale Yellow

A

Normal

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16
Q

Traumatic tap, abd. Trauma, Intra-amniotic hemorrhage

A

Blood-Streaked

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17
Q

HDN Bilirubin

18
Q

Meconium 1st fetal bowel movement

A

Dark-Green

19
Q

Fetal Death

A

Dark Red - Brown

20
Q

Ratio of Mature Fetal Lungs

21
Q

CANNOT be performed in the presence of Blood and Meconium

22
Q

Reference method for Alveolar Stability

23
Q

Serves as a control due to constant production

A

Sphingomyelin

24
Q

Amniostat FLM is an immunologic test for

A

Phosphatidylglycerol (PG)

25
NOT AFFECTED by Blood or meconium; production of PG is delayed among diabetic Mothers
Amniostat-FLM
26
Reagent Used in Foam stability/ Shake test
95% Ethanol
27
Measures by Fluorescence polarization
Microviscosity
28
Produced srufactants stored in te form of lamellar bodies
Type II pneumocytes
29
Lamellar Body Count considered to have Adequate FLM
>32,000 /uL
30
LBC can be done using IMPEDANCE or
Optical scatter
31
O.D. of _____ is equivalent to L:S ration of >=2.0 and the presence of PG
>=0.150
32
test for fetal age prior to 36 weeks of gestation
1.5 to 2.0 mg/dL amniotic fluid creatinine
33
A test for HDN which is the OLDEST ROUTINE lab test on amniotic fluid
O.D. 450
34
Normal absorbance of Amniotic Fluid
^ at 365nm < at 550nm
35
Non-affected or Mildly affected fetus
Zone I
36
Moderately affected fetus, requires careful monitoring
Zone II
37
Severly affected fetus, requires intervention: labor or EXCHANGE TRANSFUSION
Zone III
38
What is used to extract or remove hemoglobin to remove interference
Chloroform
39
it shows A450 values from 14-40 weeks of gestation
Queenan curve (Mod. Liley curve)
40
Screening test for NTD
AFP
41
Confirmatory test for NTD
Acetylcholinesterase
42
It is the major protein produced by the FETAL LIVER during early gestation (prior to 18 weeks)
AFP