Microscopic Examination of Urine Flashcards
Used to clean the optical surfaces of the microscope
Lens paper
Used to remove dust on the optical surface of the microscope
Camel hair brush
Performs initial focusing used for LPO and SO
Coarse Adjustment Knob
Most commonly used supravital stain
Identifies WBC, EPITHELIAL CELLS and CAST
Sternheimer-Malbin (SM)
Differentiates WBC and RTE cells. Enhances Nuclear detail
Toluidine blue
Distinguish RBCs from WBC, YEAST, OIL DROPLETS & CRYSTALS
Lyses RBC
2% Acetic acid
Hansel stain is composed of
Eosin Y + Methylene blue
Stains DNA
Phenathridine (Orange)
Stains NUCLEAR MEMBRANE, MITOCHONDRIA & CELL MEMBRANES
Carbocyanine (Green)
normal value of RBCs
0-2 or 0-3 HPF
(Average No.)
causes dimorphic w/ projections, Fragmented
Glomerular membrane damage
Sources of Error RBC cells
‘‘MAYO’’
Yeast
Oil droplets
Air bubbles
Monohydrate calcium oxalate crystals
Normal value of WBCs
0-5 or 0-8/ HPF
Neutrophils undergo BROWNIAN MOVEMENT, producing GLITTER CELLS in
Hypotonic urine
Normal value of Eosinophil
1%
In using Sternheimer-Malbin stain Glitter cells appear
Pale Blue
In using Sternheimer-Malbin stain LEUKOCYTES appear
Pale Pink
It is normally present in sMALL numbers
Mononuclear cells
What cell increases in Renal transplant rejection
Lymphocytes
Increase monocyte and Histiocytes are seen in
Chronic Inflammation & Radiation theraphy
Largest cell which is the point of reference w/ abundant irregular cytoplasm and prominen nucleus.
May appear folded, resenbling a cast
S.E.C
SEC covered with Gardenella vaginalis. Associated w. bacterial vaginosis
Clue cells
Spherical, polyhedral or Caudate w/ CENTRALLY located nucleus. 4-6x the size of RBC
TEC
RTE cells has an _____Nucleus
ECCENTRIC
origin of RTE cells
Nephron
> 2 RTE/ HPF indicates
Tubular Injury
Oval fat body id identified by
Lipd stains (TAG and Neutral fats)
Polarizing Microscope (cholesterol ‘‘MALTESE CROSS’’ formation
Highly refractile RTE cell seen in Lipiduria
OFB
injured cells in which the endoplasmic reticulum has dilated prior to cell death: seen in ACUTE TUBULAR NECROSIS
Bubble cell
what differentiates bacteria from amorphous urates and phosphates
Motility
if not moving T. vaginas may resemble
WBC/TEC/RTE cell
Parasite that is asso. with Bladder cancer
S. haematobium
urinary bladder cancer Markers
Nuclear Matrix protein
Bladder tumor Antigen
