ANAEROBES Flashcards

(44 cards)

1
Q

provide anaerobic environment

A

GASPAK jar

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2
Q

Substance added to provide oxygen free environment

A

Pallidum catalyst

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3
Q

Indicators used for anaerobic bacteria

A

Rezusarin
Methylene blue

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4
Q

Emit foul odor

A

C. Difficile
Fusobacterium
Porphyromonas

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5
Q

Brick red fluorescence; black pigment

A

Porphyromonas
Prevotella

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6
Q

Vellonella

A

Red fluorescence; rapidly fade

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7
Q

Fusobacterium nucleatum

A

Chartreuse fluorescence, yellow fluorescence

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8
Q

Actinomyces, Propionibacterium, Cutibacterium, Eubacterium nodatum

A

Sulfur granules

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9
Q

Microscopic: fusobacterium nucleaticum

A

Taperred ends, toothpick appearance

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10
Q

Microscopic: Bifidobacterium

A

Bifid ends/ dog bone biscuit shaped

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11
Q

Bile tolerant, cause of aspiration pneumonia

A

Bacteroides fragilis

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12
Q

Appearance of B.fragilis microscopically

A

Pin shaped

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13
Q

B.fragilis is resistant to

A

KVC
kanamycin
Vancomycin
Colistin

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14
Q

Clinical significance of Propionibacterium

A

Anaerobic diptheroid

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15
Q

Spot indole test positive color

A

Blue/ green

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16
Q

Spot indole test (+) organism

A

Cutibacterium

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17
Q

Spot indole test (-) organism

A

Propionibacterium

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18
Q

Spot indole (-) color

19
Q

Potency disk test is same with

20
Q

Purpose of Potency disk test

A

Verify gram stain of Anaerobic
Susceptibility/Resistance

21
Q

Spot indole test reagent

A

P-dimethylaminocinnamaldehyde

22
Q

PYR reagent and organism

A

P-dimethylaminocinnamaldehyde and S. Pyogenes

23
Q

Antibiotic in Potency

A

KVC
Kanamycin - 1000
Vancomycin - 5g
Colistin - 10g

24
Q

RRR

25
SRS
Fusobacterium B.ureolyticus Veillonella
26
SSR
Clostridium
27
RSR
Porphyromonas P. Anaerobius
28
RRS
Prevotella
29
SPS Sensitive test ; SPS stands for
Sodium Polyethanol Sulfonate
30
Organism inhibited by SPS
P. Anaerobius
31
Concentration of SPS in blood culture
0.025%
32
To counter act inhibition of P. Anaerobius you should add
1% GELATIN
33
SPS: C. Tetani and C. Septicum appears
- Swarming motility (like proteus) - spore forming
34
SPS: Actinomyces israelii appears
- Molar tooth (colonies) - causes Lumpy jaw: Actinomycosis of Jaw
35
SPS: Anaerobic Staphylococcus
Peptococcus niger
36
SPS: Anaerobic streptococcus
Peptostreptococcus
37
SPS: Prevotella Porphyromonas
Brick red fluorescence
38
Prevotella is important in
Dental biofilm
39
Flexible media
Thioglycolate
40
Thioglycolate is used for
Anaerobe, Facultative anaearobe, strict aerobe
41
Thioglycollate: Sign of Growth
Turbidity
42
Thioglycolate: Anaerobic location
Bottom
43
Thioglycolate: Aerobic location
Surfac
44
Thioglycolate: Facultative anaerobe location
Dispersed