HACEK

Question 1

HACEK characteristics

Answer 1

Gram negative coccobaccili
Causes SBE

Question 2

HACEK: Normal flora of oral cavity

Answer 2

H.parainfluenzae

Question 3

HACEK: Normal flora; previously under Haemophilus

Answer 3

Aggregatibacter aphrophilus

Question 4

HACEK: Star shaped colonies, usually with A. Israeli

Answer 4

Aggregatibacter actinomycetemcommitans

Question 5

HACEK: twitching motility

Answer 5

Kingella kingae

Question 6

Kingella kingae is found in

Answer 6

Bone and heart

Question 7

HACEK: forms rosette in gram stain

Answer 7

Cardiobacterium hominis

Question 8

HACEK:

Non-fermenters
Oxizider: Forms acid under aerobic
NOT NORMAL FLORA

Answer 8

Genus Pseudomonas
Genus Stenotrophomonas
Genus Burkholderia
Genus Acinetobacter

Question 9

Most common non-fermenter

Answer 9

Pseudomonas aeruginosa

Question 10

1 ICU isolated (nosocomial infection)

Answer 10

P. Aeruginosa

Question 11

P. Aeruginosa: Clinical significance

Answer 11

Pneumonia in patient w/ cystic fibrosis
Jacuzzi/hot tub syndrome
Ecthyema gangrenosum

Question 12

P. Aeruginosa: General characteristics

Answer 12

Strict aerobe
Oxidase positive
Beta hemolytic on BAP

Question 13

P. Aeruginosa: Odor

Answer 13

Old riped grapes
Taco-tortilla

Question 14

P. Aeruginosa: Medium

Answer 14

Centrimide agar

Question 15

Non-motile and Oxidase (-)

Answer 15

Acinetobacter

Question 16

Causes Glander’s disease

Answer 16

B. Mallei/ Glander’s bacillus

Question 17

With earthy odor at ashdown media

Answer 17

B. Pseudomallei
Whitmore bacillus

Question 18

Clinical significance of Whitmore bacillus

Answer 18

Mellioidosis
Vietnam time bomb

Question 19

Hacek that has WRINKLED COLONY

Answer 19

Pseudomonas stutzeri
B. Pseudomallei

Question 20

Associated with Transfusion septicemia

Answer 20

P. Fluorescence
P. Putida

Question 21

P. Fluorescence and P.putida develop

Answer 21

Pyoverdine

Question 22

Pyoverdine and Pyocyanin is produced by

Answer 22

P. Aeruginosa

Question 23

Test to differentiate P. Fluorescence to P. Putida is

Answer 23

Gelatin Hydrolysis Test
Fluorescence (+)
Putida (-)

Question 24

Component of Hugh and Leifson (OF)

Answer 24

High CHO
Low peptone

Question 25

Glucose is

Answer 25

Fermentable CHO

Question 26

Ph indicator used for OF (hugh and leifson)

Answer 26

Bromthymol blue

Question 27

2 tubes used for OF

Answer 27

Open - aerobic
Closed - anaerobic

Question 28

Result of OF: Positive

Answer 28

Yellow

Question 29

Result of OF: Negative

Answer 29

Blue/Blue green

Question 30

Indicator of OF using CDC Method

Answer 30

Phenol Red

Question 31

TSI media is used for what genus

Answer 31

Enterobacteriaceae

Question 32

Enterobacteriaceae characteristics:

Answer 32

Facultative anaerobe
All are fermenters

Question 33

Used to detect acid production and H2s production

Answer 33

Triple Sugar Iron

Question 34

TSI: 3 fermentable CHO for acid production

Answer 34

1 glucose
10 lactose
10 Sucrose

Question 35

TSI: Indicator used for acid production

Answer 35

Phenol red

Question 36

TSI: indicator used in H2s production

Answer 36

Ferrous sulfate
Sodium thiosulfate

Question 37

TSI RESULT: Acidic

Answer 37

Yellow

Question 38

TSI RESULT: Alkaline

Answer 38

RED

Question 39

TSI Reporting: Non-fermenters

Answer 39

Red slant/butt
K/K

Question 40

TSI Reporting: Non-Lactose Fermenter

Answer 40

Red slant/ yellow butt
K/A

Question 41

TSI Reporting: Lactose Fermenters

Answer 41

Yellow slant/Butt
A/A

Question 42

TSI: Can produce acid under aerobic condition, Non-fermenters (K/K)

OF: acid under aerobic (+) Open; (-) close

Answer 42

P. Aeruginosa

Question 43

Glucose (+), Facultative anaerobe, All fermenters

TSI: A/A, K/A
OF: (+) Open; (+) closed

Answer 43

Enterobacteriaceae