Analysis of Single Nucleotide Polymorphism

Question 1

SNPs occur every ____ in the genome and the majority are thought to be of no clinical significance as they occur in ____ and do not result in amino acid substitutions within coding DNA. Majority of SNPs are therefore considered ____.

Answer 1

200-500 bp; non - coding DNA; silent mutations

Question 2

SNP detection can be carried out with what methods?

Answer 2

PCR and sequencing of the region that contains it; by the use of mixture of two slightly different DNA probes (each with a different attached fluorescent label) that are designed to bind either the normal or the variant sequence, respectively.

Question 3

An increasing number of SNPs, have been found to be associated with modest (usually less than twofold) increases in an individual ’ s risk of developing common medical conditions such as ____. In addition, the presence or absence of various SNPs can be associated with altered ____.

Answer 3

type 2 diabetes; pharmacokinetics and drug responses