Analytical Instrumentation

Question 1

works like a prism and separates the light into its component wavelengths

Answer 1

diffraction grating

Question 2

the _____________ measures the transmittance and absorbance of the sample

Answer 2

detector

Question 3

the detector measures the _________________ and ____________ of the sample

Answer 3

transmittance, absorbance

Question 4

It refers to the amount of light that passes completely through the sample and strikes the detector

Answer 4

Transmittance

Question 5

It is a measurement of light that is absorbed by the sample

Answer 5

Absorbance

Question 6

The detector senses the light being transmitted through the sample and converts this information into a _____________________

Answer 6

digital display

Question 6

It is the study of how light interact with matter

Answer 6

Spectroscopy

Question 7

This works because lights gets absorbed by matter

Answer 7

Spectrophotometry

Question 8

higher concentration = light absorbed?

Answer 8

more light gets absorbed

Question 9

higher concentration = light transmitted?

Answer 9

less lights gets transmitted

Question 10

Components of spectrophotometry:

Answer 10

light source
collimator (lens)
monochromator (prism)
wavelength selector
sample dilution
detector
digital display

Question 11

This measures the incoming light and the result gets displayed

Answer 11

Detector

Question 12

This value is known as the absorbance value which is also often called?

Answer 12

optical density or od

Question 13

This value that gets displayed is known as the?

Answer 13

absorbance value

Question 14

It shows us how much light and at which wavelength it gets absorbed by our sample

Answer 14

Absorption spectrum

Question 15

In the absorption spectrum, the y-axis is the?

Answer 15

absorbance

Question 16

In the absorption spectrum, the x-axis is the?

Answer 16

wavelength

Question 17

To calculate the specific concentration of the solution used in the spectrometer, we use?

Answer 17

Beer’s law

Question 18

This can be used to quantify the amount or a substance in a solution

Answer 18

Spectrophotometry

Question 19

It is especially useful when we want to measure the change in concentration of a solution over time

Answer 19

Spectrophotometry

Question 20

It is widely used in chemistry, physics, biochemistry, and materials and chemical engineering

Answer 20

Spectrophotometry

Question 21

It is typically used to measure wavelengths of electromagnetic radiation (light) that have interacted with a sample

Answer 21

spectrometer

Question 22

It is a chromatography technique in which separation is carried out in column

Answer 22

Column Chromatography

Question 23

The column is filled with the stationary phase which can either be?

Answer 23

silica
alumina
cellulose

Question 24

The bottom of the column is packed with ______________ to support the stationary phase

Answer 24

cotton or glass wool

Question 25

The column preparation is usually carried out by two methods:

Answer 25

wet method dry method

Question 26

In this method, the stationary phase is mixed with mobile phase to make a slurry and then packed in the column

Answer 26

wet method

Question 27

In this method, the powder of the stationary phase is directly added to the column.

Answer 27

dry method

Question 28

mobile phase = ?

Answer 28

eluent

Question 29

The mobile phase leaving the column is known as

Answer 29

eluate

Question 30

The sample leaving the column is known as

Answer 30

eluite

Question 31

Other forms of column chromatography:

Answer 31

adsorption chromatography Ion exchange chromatography affinity chromatography gel permeation chromatography

Question 32

If stationary phase is adsorbent material like silica and alumina, then its known as

Answer 32

adsorption chromatography

Question 33

Is stationary phase is ion exchange resin, then its known as I

Answer 33

on exchange chromatography

Question 34

If the stationary phase is attached with the ligand that binds specific molecules such as protein or enzyme, then it is called as

Answer 34

affinity chromatography

Question 35

If the stationary phase is a porous gel or a bead, then its known as

Answer 35

gel permeation chromatography

Question 36

It is based on adsorption chromatography

Answer 36

thin layer chromatography

Question 37

Adsorbent (silica, alumina) is coated on?

Answer 37

glass slide plastic surface inert solid surface

Question 38

Thickness of adsorbent material = ?

Answer 38

0.1 mm to 0.3 mm

Question 39

Once adsorbent material is coated, the plates are kept in?

Answer 39

oven

Question 40

Coloured samples can be visualized directly

Answer 40

thin layer chromatography

Question 41

It is usually used to detect analytes that gives fluorescence

Answer 41

UV light