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MLS111 > ANAPHY LAB MICROSCOPY L1 > Flashcards

ANAPHY LAB MICROSCOPY L1 Flashcards

1
Q

7 DEFINITION OF TERMS OF MICROSCOPY

A
  1. MAGNIFICATION
  2. RESOLUTION/RESOLVING POWER
    3.NUMERICAL APERTURE
    4.FOCAL LENGTH
    5.WORKING DISTANCE
    6.PARFOCAL
    7.REFRACTIVE INDEX
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2
Q

Bending of light rays away from the objective lens when light passes from the glass of the
microscope slide to the air.

A

Refractive Index

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3
Q

Refers to quality of the objectives & eyepiece where practically no change in focus has to be
made when objective is substituted for another

A

PARFOCAL

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4
Q

Distance between the front lens of the objective lens & the top of the cover glass when the
specimen is in focus

A

WORKING DISTANCE

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5
Q
  • Thickness of the object that maybe seen at one time under focus.
  • Distance between the center of a lens or curved mirror and its focus
A

FOCAL LENGTH

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6
Q

A measurement of the ability of the condenser and the objective lens to gather light.

A

NUMERICAL APERTURE

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7
Q

MEANING OF LPO HPO OIO

A

LOW POWER OBJECTIVE
HIGH POWER OBJECTIVE
OIL IMMERSION OBJECTIVE
SCANNER

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8
Q

MAGNIFICATION OF SCANNER AND NUMERICAL APERTURE

A

4X; 0.10

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9
Q

MAGNIFICATION OF LPO AND N.A

A

10X; 0.25

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10
Q

MAGNIFICATION AND N.A OF HPO

A

40X; 0.65

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11
Q

MAGNIFICATION AND N.A OF OIO

A

100X; 1.25

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12
Q

4 TYPES OF MICROSCOPE

A

SIMPLE
COMPOUND
SCANNING E
TRANSMISSION E

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13
Q

Created in the 17th century by Antony van
Leeuwenhoek, who combined a convex lens with a
holder for specimens.

200 – 300x Magnification

A

SIMPLE

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14
Q

Two lenses

Better magnification than a simple microscope

A

COMPOUND MICROSCOPE

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15
Q

the specimen is lit from
underneath, and they can be binocular or monocular)
provide a magnification of 1,000 times, which is
considered to be high, although the resolution is low.

A

BRIGHT FIELD

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16
Q

Stained tissue is examined with ordinary light
passing through the preparation.

A

BRIGHT FIELD

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17
Q

TYPES OF COMPOUND MICROSCOPE

A

BRIGHT FIELD MICROSCOPE
FLUORESCENCE MICROSCOPY
PHASE-CONTRAST MICROSCOPY
POLARIZING MICROSCOPY

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18
Q

Tissue sections are usually irradiated with
ultraviolet (UV) light and the emission is in the
visible portion of the spectrum.

A

FLUORESCENCE MICROSCOPY

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19
Q

Fluorescent substances appear bright on a dark
background

A

FLUORESCENCE MICROSCOPY

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20
Q

uses a lens system that produces visible images
from transparent objects and, importantly, can be
used with living, cultured cells

A

PHASE-CONTAST MICROSCOPY

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21
Q

Allows the recognition of stained or
unstained structures made of highly
organized subunits.

A

POLARIZING MICROSCOPY

22
Q

Tissue structures containing oriented
macromolecules are located between the
two polarizing filters, they appear as
bright structures against a dark
background.

A

POLARIZING MICROSCOPY

23
Q

type of microscope that uses visible light and an objective lens to magnify and illuminate a sample.

A

BF MICROSCOPY

24
Q

Type of optical microscopy where a fluorescent sample is illuminated with light of certain wavelength

A

FLUORESCENCE

25
Q

a light microscopy technique used to enhance the contrast of images of transparent and colourless specimens.
BLACK AND WHITE

A

PHASE-CONTRAST

26
Q

a microscope that utilizes polarized light to reveal detail in an object, used especially to study crystalline and fibrous structures.

A

POLARIZING

27
Q
  • Uses electrons rather than light for image
    formation.
  • Samples are scanned in vacuum or near-
    vacuum conditions, so they must be specially
    prepared by first undergoing dehydration and
    then being coated with a thin layer of a
    conducive material, such as gold.
  • After the item is prepared and placed in the
    chamber, the SEM produces a 3-D, black-and-
    white image on a computer
A

SEM

28
Q

-Uses electrons in creating a magnified image

  • Uses a slide preparation to obtain a 2-D view
    of specimens, so it’s more suited for viewing
    objects with some degree of transparency.
  • Offers a high degree of both magnification and
    resolution
A

TEM

29
Q

3 GEN PART OF MICROSCOPE

A

MECHANICAL
MAGNIFYING
ILLUMINATING

30
Q

used to support and adjust the parts

A

MECHANICAL

31
Q

USED TO ENLARGE SPECIMEN

A

MAGNIFYING

32
Q

USED TO PROVIDE LIGHTS

A

ILLUMINATING

33
Q

post portion that supports the entire
microscope, provides stability and support for the
microscope when it is upright. also typically
holds the illuminator, or light source

A

BASE

34
Q

part above the base that supports the other parts

A

PILLAR

35
Q

allows for tilting for the convenience
of the user

A

INCLINATION JOINT

36
Q

connects the eyepiece tube to the base ,curve or
slanted part which is held while carrying the
microscope

A

ARM - CARRYING HANDLE

37
Q

platform for the slides,
which hold the specimen. The stage typically has a
stage clip on either side to hold the slide firmly in
place. Some microscopes have a mechanical stage,
with adjustment knobs that allow for more precise
positioning of slides

A

Stage and stage clip

38
Q

attached to the arm and bears the lenses

A

BODY TUBE

39
Q

cylindrical structure on the top of the
body tube that holds the ocular lenses

A

Draw tube

40
Q

contains the objective
lenses. Microscope users can rotate this part to
switch between the objective lenses and adjust the
magnification power

A

REVOLVING NOSE PIECE

41
Q

Lies atop the nose piece and keeps dust
from settling on the objectives

A

DUST SHIELD

42
Q

moves
stage (or body tube) up and down, used
to focus the image on the
microscope under low or medium
power

A

Coarse Adjustment Knob

43
Q

small knob,
used to fine-tune the focus of your
specimen after using the coarse
adjustment knob

A

Fine Adjustment Knob

44
Q

ontains the ocular lens,
which the user looks through to see the
magnified specimen, further magnify the
image produced by the objective lenses by 5x
to 15x

A

EYEPIECE/OCULAR

45
Q

combine with the eyepiece
lens to increase magnification levels.
Microscopes generally feature three or four
objective lenses, with magnification levels
ranging 4x to 100x

A

OBJECTIVE LENSES

46
Q

light source for viewing. This can come
in the form of a built-in, low-voltage illuminator
light, or a mirror that reflects an external light
source like sunlighT

A

ILLUMINATOR/ E-LAMP

47
Q

has a concave and plane surface to
gather and direct light in order to illuminate the
object

A

MIRROR

48
Q

focus the light onto the specimen,
concentrating light rays on the specimen

A

CONDENSER

49
Q

controls the amount of light
reaching the specimen and regulates the light
necessary to obtain a clear view of the objecT

A

IRIS DIAPHRAGM

50
Q

USING MICROSCOPE

A

The proper way to focus a microscope is to start with the lowest power objective lens first
and while looking from the side, crank the lens down as close to the specimen as possible
without touching it.
* Look through the eyepiece lens and focus upward only until the image is sharp. If you can’t
get it in focus, repeat the process again.
* Once the image is sharp with the low power lens, you should be able to simply click in the
next power lens and do minor adjustments with the focus knob.
* If your microscope has a fine focus adjustment, turning it a bit should be all that’s
necessary. Continue with subsequent objective lenses and fine focus each time.

51
Q

CARE OF THE MICROSCOPE

A

-Always carry with 2 hands
* Never touch the lenses with your fingers.
* Only use lens paper for cleaning
* Keep objects clear of desk and cords
* When you are finished with your “scope”, rotate the nosepiece so that it’s
on the low power objective, roll the stage down to lowest level, rubber
band the cord, then replace the dust cover.

MLS111 (13 decks)

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