ANAPHY LAB MICROSCOPY L1

Question 1

7 DEFINITION OF TERMS OF MICROSCOPY

Answer 1

1. MAGNIFICATION
2. RESOLUTION/RESOLVING POWER
   3.NUMERICAL APERTURE
   4.FOCAL LENGTH
   5.WORKING DISTANCE
   6.PARFOCAL
   7.REFRACTIVE INDEX

Question 2

Bending of light rays away from the objective lens when light passes from the glass of the
microscope slide to the air.

Answer 2

Refractive Index

Question 3

Refers to quality of the objectives & eyepiece where practically no change in focus has to be
made when objective is substituted for another

Answer 3

PARFOCAL

Question 4

Distance between the front lens of the objective lens & the top of the cover glass when the
specimen is in focus

Answer 4

WORKING DISTANCE

Question 5

• Thickness of the object that maybe seen at one time under focus.
• Distance between the center of a lens or curved mirror and its focus

Answer 5

FOCAL LENGTH

Question 6

A measurement of the ability of the condenser and the objective lens to gather light.

Answer 6

NUMERICAL APERTURE

Question 7

MEANING OF LPO HPO OIO

Answer 7

LOW POWER OBJECTIVE
HIGH POWER OBJECTIVE
OIL IMMERSION OBJECTIVE
SCANNER

Question 8

MAGNIFICATION OF SCANNER AND NUMERICAL APERTURE

Answer 8

4X; 0.10

Question 9

MAGNIFICATION OF LPO AND N.A

Answer 9

10X; 0.25

Question 10

MAGNIFICATION AND N.A OF HPO

Answer 10

40X; 0.65

Question 11

MAGNIFICATION AND N.A OF OIO

Answer 11

100X; 1.25

Question 12

4 TYPES OF MICROSCOPE

Answer 12

SIMPLE
COMPOUND
SCANNING E
TRANSMISSION E

Question 13

Created in the 17th century by Antony van
Leeuwenhoek, who combined a convex lens with a
holder for specimens.

200 – 300x Magnification

Answer 13

SIMPLE

Question 14

Two lenses

Better magnification than a simple microscope

Answer 14

COMPOUND MICROSCOPE

Question 15

the specimen is lit from
underneath, and they can be binocular or monocular)
provide a magnification of 1,000 times, which is
considered to be high, although the resolution is low.

Answer 15

BRIGHT FIELD

Question 16

Stained tissue is examined with ordinary light
passing through the preparation.

Answer 16

BRIGHT FIELD

Question 17

TYPES OF COMPOUND MICROSCOPE

Answer 17

BRIGHT FIELD MICROSCOPE
FLUORESCENCE MICROSCOPY
PHASE-CONTRAST MICROSCOPY
POLARIZING MICROSCOPY

Question 18

Tissue sections are usually irradiated with
ultraviolet (UV) light and the emission is in the
visible portion of the spectrum.

Answer 18

FLUORESCENCE MICROSCOPY

Question 19

Fluorescent substances appear bright on a dark
background

Answer 19

FLUORESCENCE MICROSCOPY

Question 20

uses a lens system that produces visible images
from transparent objects and, importantly, can be
used with living, cultured cells

Answer 20

PHASE-CONTAST MICROSCOPY

Question 21

Allows the recognition of stained or
unstained structures made of highly
organized subunits.

Answer 21

POLARIZING MICROSCOPY

Question 22

Tissue structures containing oriented
macromolecules are located between the
two polarizing filters, they appear as
bright structures against a dark
background.

Answer 22

POLARIZING MICROSCOPY

Question 23

type of microscope that uses visible light and an objective lens to magnify and illuminate a sample.

Answer 23

BF MICROSCOPY

Question 24

Type of optical microscopy where a fluorescent sample is illuminated with light of certain wavelength

Answer 24

FLUORESCENCE

Question 25

a light microscopy technique used to enhance the contrast of images of transparent and colourless specimens. BLACK AND WHITE

Answer 25

PHASE-CONTRAST

Question 26

a microscope that utilizes polarized light to reveal detail in an object, used especially to study crystalline and fibrous structures.

Answer 26

POLARIZING

Question 27

* Uses electrons rather than light for image formation. * Samples are scanned in vacuum or near- vacuum conditions, so they must be specially prepared by first undergoing dehydration and then being coated with a thin layer of a conducive material, such as gold. * After the item is prepared and placed in the chamber, the SEM produces a 3-D, black-and- white image on a computer

Answer 27

SEM

Question 28

-Uses electrons in creating a magnified image - Uses a slide preparation to obtain a 2-D view of specimens, so it's more suited for viewing objects with some degree of transparency. - Offers a high degree of both magnification and resolution

Answer 28

TEM

Question 29

3 GEN PART OF MICROSCOPE

Answer 29

MECHANICAL MAGNIFYING ILLUMINATING

Question 30

used to support and adjust the parts

Answer 30

MECHANICAL

Question 31

USED TO ENLARGE SPECIMEN

Answer 31

MAGNIFYING

Question 32

USED TO PROVIDE LIGHTS

Answer 32

ILLUMINATING

Question 33

post portion that supports the entire microscope, provides stability and support for the microscope when it is upright. also typically holds the illuminator, or light source

Answer 33

BASE

Question 34

part above the base that supports the other parts

Answer 34

PILLAR

Question 35

allows for tilting for the convenience of the user

Answer 35

INCLINATION JOINT

Question 36

connects the eyepiece tube to the base ,curve or slanted part which is held while carrying the microscope

Answer 36

ARM - CARRYING HANDLE

Question 37

platform for the slides, which hold the specimen. The stage typically has a stage clip on either side to hold the slide firmly in place. Some microscopes have a mechanical stage, with adjustment knobs that allow for more precise positioning of slides

Answer 37

Stage and stage clip

Question 38

attached to the arm and bears the lenses

Answer 38

BODY TUBE

Question 39

cylindrical structure on the top of the body tube that holds the ocular lenses

Answer 39

Draw tube

Question 40

contains the objective lenses. Microscope users can rotate this part to switch between the objective lenses and adjust the magnification power

Answer 40

REVOLVING NOSE PIECE

Question 41

Lies atop the nose piece and keeps dust from settling on the objectives

Answer 41

DUST SHIELD

Question 42

moves stage (or body tube) up and down, used to focus the image on the microscope under low or medium power

Answer 42

Coarse Adjustment Knob

Question 43

small knob, used to fine-tune the focus of your specimen after using the coarse adjustment knob

Answer 43

Fine Adjustment Knob

Question 44

ontains the ocular lens, which the user looks through to see the magnified specimen, further magnify the image produced by the objective lenses by 5x to 15x

Answer 44

EYEPIECE/OCULAR

Question 45

combine with the eyepiece lens to increase magnification levels. Microscopes generally feature three or four objective lenses, with magnification levels ranging 4x to 100x

Answer 45

OBJECTIVE LENSES

Question 46

light source for viewing. This can come in the form of a built-in, low-voltage illuminator light, or a mirror that reflects an external light source like sunlighT

Answer 46

ILLUMINATOR/ E-LAMP

Question 47

has a concave and plane surface to gather and direct light in order to illuminate the object

Answer 47

MIRROR

Question 48

focus the light onto the specimen, concentrating light rays on the specimen

Answer 48

CONDENSER

Question 49

controls the amount of light reaching the specimen and regulates the light necessary to obtain a clear view of the objecT

Answer 49

IRIS DIAPHRAGM

Question 50

USING MICROSCOPE

Answer 50

The proper way to focus a microscope is to start with the lowest power objective lens first and while looking from the side, crank the lens down as close to the specimen as possible without touching it. * Look through the eyepiece lens and focus upward only until the image is sharp. If you can't get it in focus, repeat the process again. * Once the image is sharp with the low power lens, you should be able to simply click in the next power lens and do minor adjustments with the focus knob. * If your microscope has a fine focus adjustment, turning it a bit should be all that's necessary. Continue with subsequent objective lenses and fine focus each time.

Question 51

CARE OF THE MICROSCOPE

Answer 51

-Always carry with 2 hands * Never touch the lenses with your fingers. * Only use lens paper for cleaning * Keep objects clear of desk and cords * When you are finished with your "scope", rotate the nosepiece so that it's on the low power objective, roll the stage down to lowest level, rubber band the cord, then replace the dust cover.