Anas Lecture (Pharmacoproteomics)

Question 1

What is “P4”?

Answer 1

-Participatory
-Personalized
-Predictive
-Preventative

Question 2

Benefits of P4 medicinal approach?

Answer 2

-Cost Effective
-Reduce Dx Incidence
-Better Pt. Outcomes
-Better Pt. Compliance

Question 3

What is “pharmacoproteomics”?

Answer 3

Changes in protein abundance brought about by med administration… Examining at the protein level what a drug is doing to the body.

Question 4

Match the proteomic tech approaches to their purpose:

Gel Electrophoresis
Chromatography
Immunoassays
Mass Spectrometry

Isolation
ID
Quantification

Answer 4

GE: Isolate, ID, Qty
C: Isolate, ID, Qty
IA: ID, Qty
MS: ID, Qty

Question 5

Contrast Top Down Proteomics from Shotgun Proteomics.

Answer 5

TD: Run proteins through gel using SDS-PAGE, chop up gel into strips, instill into wells & expose proteins to Trypsins, then use LC-MS Analysis to quantify & ID target proteins.

Shotgun: Take entire protein sample & expose to Trypsins… Then, straight to LC-MS. No need for chromatography steps!

Question 6

Can we isolate proteins from Immunoassays?

Answer 6

NOOOOOO… Only quantify & ID.

Question 7

What is Mass Spectrometry?

Answer 7

Ionizer imparts a charge to gas phase ions, which are then quantified and detected according to their mass-to-charge (m/z) ratios.

Question 8

What is Toxicoproteomics?

Answer 8

Analyzes how chem exposure modifies proteins or overall protein expression levels.