Animal Cloning Flashcards
(34 cards)
is a technique used for direct genetic modification of organisms using recombination of DNA
Genetic engineering
purpose is direct manipulation of DNA sequences
Genetic engineering
these techniques involve the capacity to isolate, cut, and transfer specific DNA pieces, corresponding to specific genes
Genetic engineering
a Finn Dorsett ewe, was the first mammal to have been successfully cloned from an adult cell
Dolly
is the creation of an organism that is an exact genetic copy of another
Cloning
Procedure of isolating a defined DNA sequence and obtaining multiple copies of it in vivo
DNA cloning
The objectives of _____ DNA :
– Identifying genes
– Isolating genes
– Modifying genes
– Re-expressing genes in other hosts or
organisms
Recombinant DNA
is a piece of DNA that is capable of independent growth (bacterial plasmids and
viral phages)
Vector
is used to cut a specific DNA strand from the DNA of a cell
Restriction enzyme
is used to bond the DNA fragment with the virus’ DNA
DNA Ligase
is used to amplify a single molecule of DNA into many copes
Vector
is a DNA molecule that has an origin of replication and is capable of replicating in a bacterial cell
Cloning vector
They can replicate independently of the host cell
Plasmid
circular, double-stranded DNA molecules
Plasmid
they have an origin of replication, antibiotic resistance genes as markers, and several unique restriction sites.
Plasmids
are small circles of DNA found in bacteria
Plasmids
This process entails the transfer of a nucleus from a donor adult cell to an egg which has no nucleus
Reproductive cloning
is the production of human embryos for use in
research
Therapeutic cloning
The goal of this process is not to create cloned human beings, but rather to harvest stem cells that can be used to study human development and to treat disease
Therapeutic cloning
this can also be used to create a clonal embryo
Somatic cell nuclear transfer
fusing two cells by electric shock
Electrofusion
Can be implanted into a uterus of a female to create a whole organism
Blastomer
can also be used as a source of stem cells
Blastomeres
As a consequence, clones such as Dolly that are born from ____ are not perfect copies of the donor of the nucleus
SCNT