Antibodies as diagnostic tools Flashcards Preview

MCD Y2: Laboratory diagnostic methods > Antibodies as diagnostic tools > Flashcards

Flashcards in Antibodies as diagnostic tools Deck (13):
1

Why is it inefficient to collect antibodies from animals?

They have a polyclonal response to antigen

2

Summarise the production of monoclonal antibodies

Take B cell producing correct Ab --> fuse with myeloma cell line --> hybridoma --> Ab of interest produced

3

Describe the production of antibody using recombinant DNA technology

1. Isolate genes encoding Ab variable regions
2. Construct fusion protein of V region with bacteriophage protein coat
3. Clone random populatio of phages to produce phage display library
4. Select phage with desired V region and amplify

4

What is implicated by the suffix of an antibody being "omab"?

mouse monoclonal

5

What is implicated by the suffix of an antibody being "imab"?

chimeric or partly humanised

6

What is implicated by the suffix of an antibody being "umab"?

human

7

Recall 4 therapeutic uses of antibodies

1. Cancer drug (herceptin)
2. In immunodeficiency
3. Blocking cytokine activity
4. Removal of T cells from bone marrow grafts

8

Recall 4 things that can be diagnosed using antibodies

Infection
AI disease
Allergy
Malignancy

9

Recall 3 antibody diagnostic methods

Tissue typing
Serology
Immunoassays

10

Describe how ELISA works

2 samples containing Ag used to coat inside of “wells” --> wells covered in Ab against Ag to be measured
If Ag is present, Ab will adhere to well wall -
Reporter is enzyme --> colour change

11

What is the main use for flow cytometry?

Identify lymphocyte type using Ab against CD markers

12

What can serum electrophoresis be used to show?

Monoclonal expansion of B cells

13

Give 3 examples of things ELISA might be used to look for

Tetanus
Hameophillus
Pneumococcus