Diagnostics: virology lab Flashcards Preview

MCD Y2: Laboratory diagnostic methods > Diagnostics: virology lab > Flashcards

Flashcards in Diagnostics: virology lab Deck (14):
1

Describe the difference between the sensitivity and specificity of a test

Sensitivity = ability to detect false positives
Specificity = ability to detect false negatives

2

Recall 3 ways of sampling for respiratory viruses

Bronchealveolar lavage
Nasopharyngeal aspirate
Throat swab

3

What viruses can be tested for using a stool sample?

Entovirus
Adenovirus
Rotavirus

4

What viruses can be identified in urine?

BK
Adenovirus

5

What viruses can be detected in the CSF?

Herpes
Enterovirus

6

How are IgM and IgG measured?

Serology

7

What viruses can be tested for using the results of an IgG/ IgM test?

CMV
EBV
MMR
Parvovirus

8

What additional test is required for all new confirmed HIV+s?

EDTA blood to test viral load

9

How is viral load assessed?

Blood (EDTA)

10

What is the most common use of immuofluorescence in testing for viruses?

Out-of-hours respiratory virus testing

11

how can PCR be used in virus testing?

Can amplify DNA/ RNA sequences from a dsDNA starting block using RT

12

What is the enzyme used to amplify RNA samples in PCR?

Taq polymerase

13

What is the main advantage of multiplex PCR?

Can test for many different viruses simultaneously

14

What test is done to test for antiviral drug resistance?

Sequencing