Flashcards in Diagnostics: virology lab Deck (14):
Describe the difference between the sensitivity and specificity of a test
Sensitivity = ability to detect false positives
Specificity = ability to detect false negatives
Recall 3 ways of sampling for respiratory viruses
What viruses can be tested for using a stool sample?
What viruses can be identified in urine?
What viruses can be detected in the CSF?
How are IgM and IgG measured?
What viruses can be tested for using the results of an IgG/ IgM test?
What additional test is required for all new confirmed HIV+s?
EDTA blood to test viral load
How is viral load assessed?
What is the most common use of immuofluorescence in testing for viruses?
Out-of-hours respiratory virus testing
how can PCR be used in virus testing?
Can amplify DNA/ RNA sequences from a dsDNA starting block using RT
What is the enzyme used to amplify RNA samples in PCR?
What is the main advantage of multiplex PCR?
Can test for many different viruses simultaneously