Antigen Capture And Presentaion To Lymphocytes (Ch. 3)

Question 1

What presents Ag on cells to be recognized by T cells

Answer 1

Major Histological Complexes (MHC’s)

Question 2

What do DC’s secrete to attract T cells

Answer 2

CCR 7

Question 3

The Ln handles infection where? What about the spleen?

Answer 3

LN = tissues/lymph
Spleen = blood

Question 4

What are the two types of DC’s and where are they found

Answer 4

Classical - found in tissues (Langerhans is example)

Plasmacytoid - Blood and tissue (major source of IFN)

Question 5

What are the surface markers for classical and plasmacytoid DC’s

Answer 5

Classical = CD 11 c and b

Plasmacytoid = B220

Question 6

What TLR’s are expresses on classical and plasmacytoid DC’s

Answer 6

Classical = 4,5,8

Plasmacytoid = 7, 9

Question 7

What is produced by classical and plasmacytoid DC’s

Answer 7

Classical = cytokines IL - 6 and 16, and TNF

Plasmacytoid = Type 1 interferons

Question 8

Which DC classically activates T cells

Answer 8

Classical DC’s

Question 9

DC’s express MHC’s for what? What about macrophages? What about FDC’s

Answer 9

DC = CD4 and CD8
Mac = CD4 
FDC = B Cells

Question 10

What is the human MHC called

Answer 10

Human Leukocyte Antigens (HLA)

Question 11

Class 1 MHC has what alleles

Answer 11

HLA - A, B, and C

Two from each parent

Question 12

Class 2 MHC has what alleles

Answer 12

Varies

Question 13

HLA’s are very what in terms of gene expression? What are they in terms of receiving genes from each parent?

Answer 13

Very polymorphic

Express codomenance which means they express genes equally from each parent.

Question 14

What can only bind to MHC Class 1?

Answer 14

CD 8

Question 15

How do people get their specific MHC genes

Answer 15

They are inherited codominantly

Question 16

Class 2 MHC are mainly on what cells? What about Class 1?

Answer 16

Class 2 = DC, macrophages, B-cells

Class 1 = all cells

Question 17

Class 2 MHC binds only to what

Answer 17

CD4

Question 18

B cells can recognize class 2 MHC and also what

Answer 18

Almost anything else

Question 19

Each MHC can only present how many peptide AG at a time

Answer 19

1

Question 20

Extra-cellular proteins that are internalized by APC’s are broken down by what and displayed on what?

Answer 20

Broken down by endosomes/lysosomes and displayed as Class 2 MHC’s

Question 21

Cytosolic proteins of any nucleoid cell are broken down by what and presented as what

Answer 21

Broken down by proteases (ubiquiten pathway) and displayed as Class 1 MHC’s

Question 22

Where are MHC’s made

Answer 22

In the ER