Application of Molecular Techniques in the Diagnosis IN GENETICS AND FORENSICS

Question 1

Genetics in Molecular Biology specifically deals with the?

Answer 1

Human Chromosome

Question 2

This refers to the complete set of the chromosomes in the cells of organisms

Answer 2

Karyotyping

Question 3

It is a new technique under molecular biology

Answer 3

In situ Hybridization

Question 4

This technique before uses radiolabeled probes as target labels

Answer 4

In Situ Hybridization

Question 5

This is a tagging technique in FISH

Answer 5

Nick Translation

Question 6

It is a method on how we add the probe into the sample or reagent

Answer 6

Nick Translation

Question 7

It is a process wherein the DNA polymerase I is used to replaced some nucleotides of DNA sequences

Answer 7

Nick Translation

Question 8

Give the advanced application of FISH

Answer 8

1. Interphase FISH
2. WCP (Whole Chromosome Painting
3. MFISH (Multicolor FISH)
4. SKY (Spectral Karyotyping)
5. ALL-TELOMERE0FISH
6. Comparative Genomic Hybridization

Question 9

There is no cell culture required here, thus the diagnosis is much rapid

Answer 9

Interphase FISH

Question 10

In this application, different or many types of probes are used from a SINGLE chromosome

Answer 10

WCP (Whole Chromosome Painting)

Question 11

The main principle of this application is, on the single chromosome, there are LOTS OF UNIQUE DNA associated with it

Answer 11

WCP (Whole Chromosome Painting)

Question 12

The main advantage of this application is that it is much more automated and fixed profile

Answer 12

MFISH (Multiple FISH)

Question 13

This application is a computer based

Answer 13

MFISH (Multiple FISH)

Question 14

In this type of application, you will be able to observe multiple chromosomal aberrations or changes

Answer 14

SKY (Spectral Karyotyping)

Question 15

This application is almost the same with WCP, but instead of a single chromosome, multiple chromosomal aberrations are detected

Answer 15

SKY (Spectral Karyotyping)

Question 16

The main probe/label of this application is a telomere probe

Answer 16

ALL-Telomere FISH

Question 17

This probe is used to recognize six base repeats which is present at the end of all choromosomes

Answer 17

Telomere probe

Question 18

This application detects six base repeats sequence present at the ends of all chromosome

Answer 18

ALL-Telomere FISH

Question 19

The main advantage of this application is used as a confirmatory assay

Answer 19

ALL-Telomere FISH

Question 20

In this type of application, you simply compare the DNA content of a differentially labeled normal and abnormal cell population

Answer 20

Comparative Genomic Hybridization

Question 21

In this application, we only compare DNA content

Answer 21

Comparative Genomic Hybridization

Question 22

Give the main application of FISH

Answer 22

1. Diagnosis
2. Prognosis
3. Evaluate

Question 23

It refers to some kind of the inherited diseases associated with mutation

Answer 23

Genetics

Question 24

It is a certain condition whether there is a presence or absence of a mutated gene

Answer 24

Prognosis

Question 25

Advantages of FISH

Answer 25

1. much more specific 2. distinguish one disease from another 3. detected disease more easily 4. can be quantitated through automated methods

Question 26

It is one of the most powerful tools in the fight against crime and other legal matters

Answer 26

DNA Testing

Question 27

It is a common specimen that can be used in identifying criminal suspects in forensic

Answer 27

Hair

Question 28

Age, sex, and sometimes medical history can be determine by this

Answer 28

bones

Question 29

This evidence can be used in police and court matters

Answer 29

Fingerprint

Question 30

This is considered as a best sample to use in forensics

Answer 30

Blood

Question 31

Where can we obtain DNA?A

Answer 31

It can be obtain from blood, semen, saliva, bone, teeth, or microscopic flecks of skin

Question 32

The highest amount of DNA can be obtained in?

Answer 32

Male semen

Question 33

It is the best specimen if one wants to investigate crime associated with sexual assault

Answer 33

Semen

Question 34

True or False: Forensics may also be used in identifying issues in relation to that of the family

Answer 34

True

Question 35

How will you know if a certain sequence is a Satellite DNA?

Answer 35

As long as there are two repeating sequences