12 Double stranded RNA segments NON enevloped icosahedral

Arboviruses 3 (Reoviridae) + Rhabdo/Filo Flashcards by Chris Robert

Reoviridae genus

Coltivirus: Colorado Tick Fever Virus

How well did you know this?

Not at all

Perfectly

Coltivirus structure

12 Double stranded RNA segments
NON enevloped
icosahedral

How well did you know this?

Not at all

Perfectly

Coltivirus replication cycle (5 steps)

endocytosed, enters lysosome
core particle primary transcript
Translated into +/- strands in cytoplasm
replicase creates + strand from the - one
capsid assembly and release

How well did you know this?

Not at all

Perfectly

Colorado tick fever is a ____, _____ illness

nonspecific, febrile

How well did you know this?

Not at all

Perfectly

Coltivirus clinical findings (4)

Tick bite
Saddleback Fever
Flu-like symptoms
Transient Rash

(Complication of Encephalitis or pericarditis)

How well did you know this?

Not at all

Perfectly

Coltivirus infects _______ cells.

What effect does this have?

Bone Marrow cells

this halts development of blood cell types (PMN, eos, baso’s)

How well did you know this?

Not at all

Perfectly

When do Coltivirus symptoms occur

4-5 days after tick bite

How well did you know this?

Not at all

Perfectly

Significance of the first two weeks of Coltivirus infection

free virus can be found in the blood

after which it circulates within erythroblasts

How well did you know this?

Not at all

Perfectly

When does Coltivirus antibody appear?

two weeks after symptom onset

How well did you know this?

Not at all

Perfectly

Coltivirus lifespan in blood

can be isolated for 6 weeks

BUT can live in RBC for life of the cell

How well did you know this?

Not at all

Perfectly

Coltivirus resevoir and transmitter

Squirrel/rodent resevoir

Wood Tick is transmitter

How well did you know this?

Not at all

Perfectly

What tick spreads Coltivirus?

Rocky Mountain wood tick

(Dermacentor andersoni)

How well did you know this?

Not at all

Perfectly

90% of Coltivirus cases happen during…

april-july

How well did you know this?

Not at all

Perfectly

Coltivirus control

stay away from ticks

check yo pets

get out of your skin if embedded

How well did you know this?

Not at all

Perfectly

Filoviridae diseases

Ebola

Marburg

How well did you know this?

Not at all

Perfectly

Characteristics that filoviridae share

Genome structure

Zoonotic

Damage the microvasculature = hemorrhage

How well did you know this?

Not at all

Perfectly

Filovirus organism structure

ss (-) RNA

Encodes 7 proteins

Enveloped filaments

Helical

How well did you know this?

Not at all

Perfectly

Filovirus nucleocapsid enclosed in an envelope that contains…

one glycoprotein

How well did you know this?

Not at all

Perfectly

Filovirus replicates in the

cytoplasm

How well did you know this?

Not at all

Perfectly

Filovirus (ebola) causes ______ infection

hemorrhagic

How well did you know this?

Not at all

Perfectly

Ebola rash appearance and time

Study These Flashcards

macropapular, appears at 5 days

Signs of recovery occur at day ____ and are concurrent with…

Study These Flashcards

Day 7

With antibody response

Ebola transmission and reservoir

Study These Flashcards

Unknown

possibly bats

Major characteristic of Ebola (filo) infection is…

Study These Flashcards

destruction of the immune system

especially the breakdown of endothelial cells

Ebola cell damage due to \_\_\_\_

Cytokine release from Monocytes

Ebola cytokine dysregulation promotes ______ and \_\_\_\_\_\_

hemorrhage + vasomotor collapse

Ebola vector

Not identified

Rotavirus serogroups

A-E

Rotavirus genome and structure

**nonenveloped** **dsRNA** 3 capsid layers + inner core (Outer capsid VP4)

Rotavirus VP4 protein is located on _____ and functions to aid \_\_\_\_\_\_\_

Outer capsid ## Footnote **host attachment**

Is rotavirus segmented or not?

Yes **_11_** segments of **dsRNA**

Rotavirus transcription and translation occur in \_\_\_\_\_\_\_

Inner core = transcription Cytoplasm = translation

What is different about the replication of Rotavirus?

replicates in **progeny** core uses **positive** RNA template

Rotavirus release from cell, two steps...

buds into ER release by cell lysis

Rhabdovirus genus

Lyssavirus

Rhabdo shape and structure

bullet shaped helical, *enveloped* ## Footnote **ss (-) RNA**

Rhabdo: associated proteins

NPMGL (nucleo, phospho, matrix, glyco, polymerase)

Rhabdo replication

1. Binds using **G protein** 2. **Pinocytosis** into cytoplasm 3. Endosome fusion and release RNP into cytoplasm 4. L gene (pol-ase) makes **5 mRNA**s + 1 full **(+)RNA** 5. **Translation** of NPMGL (G = processed in ER and Golgi) 6. +RNA serves as templates for full length (-) RNA 7. **Assemble NPL** around RNP core, M forms capsule (matrix) 8. **Bud** from PM and release

Rhabdo periods (and # of days for each)

**Incubation** (20-90) **Prodromal** (2-10) **Neurological** (2-7)

Rhabdo travels via..

retrograde axonal transport

Rhabdo pathogenesis duration depends on...

**size & site** of exposure human/animal **immunity**

Rhabdo replicates in \_\_\_\_\_

muscle at the site of innoculation

Rhabdo concentrates in \_\_\_\_\_\_

salivary glands

common reservoirs for rhabdo

bats, foxes, raccoons, skunks

Rhabdo Dx

Histopathology (also electron micro, direct elisa/immunochemistry)

signs of rhabdo in histopathology are... (5)

1. mononuclear infiltration 2. perivascular cuffing (lymph/PMN) 3. Lymphocytic foci 4. Babes nodules 5. **_NEGRI BODIES_**

Rhabdo vaccine is unique in that it is adminitered

Post exposure

Other measures taken after exposure to rhabdo (other than post exposure vax)

Clean site with soap and water

Rhabdo vax administration schedule after exposure?

Days - 0, 3, 7, 14, 28

Is there a pre exposure rhabdo vaccine?

Yes 3 doses

What is the Wisconsin rabies treatment protocol?

Ketamine Ribavirin Amantadine

Arboviruses 3 (Reoviridae) + Rhabdo/Filo Flashcards

(51 cards)