Papillomaviridae & Polyomaviridae

Question 1

Papillomavirus structure

Answer 1

dsDNA (Circular)

Nonenveloped

icosahedral capsid

Question 2

Papillomavirus capsid proteins

Answer 2

L1 + L2

L1 = 80% of virion

L2 = Minor, makes up the rest

Question 3

There are _____ types of Papillomavirus

Answer 3

100 types

*grouped into 16 groups (A-P)

Question 4

Papillomavirus strains are classified as _____ or _____

Answer 4

high or low risk

Question 5

Cervical cancer is consistent with HPV in _____% of cases

Answer 5

75%

Question 6

Papillomavirus replication occurs in the _____ and assembly occurs in the ______

Answer 6

nucleus, nucleus

Question 7

HPV replicates in ______ cells

Answer 7

differentiated skin cells

Question 8

_____ genes stimulate HPV division and growth

Answer 8

Early genes

Question 9

Based on TISSUE tropism, HPV’s are classified into _____ or ______

Answer 9

cutaneous or mucosal

Question 10

Cutaneous HPV caused by strains…

Answer 10

1, 2, 3, 4

Question 11

Cutaneous HPV incubation time

Answer 11

3-4 months

Question 12

Mucosal HPV known to cause…

Answer 12

Single oral papillomas

Laryngeal papillomas

Question 13

Most common benign epithelial tumors of the oral cavity are…

Answer 13

Single Oral Papillomas (Mucosal HPV)

Question 14

Single oral papillomas features (2)

Answer 14

occur at any age

rarely recur

Question 15

Laryngeal papillomas are associated with strains ______ and ____

Answer 15

6 + 11

Question 16

Which mucosal papillomas are likelier to recur?

Answer 16

Laryngeal papillomas

Question 17

A-G warts are caused by

Answer 17

condylomata acuminata

Question 18

90% of AGW’s are associated with HPV___ and ____

Answer 18

6+11

Question 19

HPV strains associated with infection of the female genital tract:

Answer 19

16, 18, 31, 45

Question 20

85% of cervical carcinoma contains _______

Answer 20

integrated DNA from HPV

Question 21

Explain oncogenesis in terms of cervical dysplasia and HPV

Answer 21

E6 +E7 are oncogenic, and normally inhibited by E1+E2

E2 is broken to promote DNA integration

6 + 7 are free to bind p53 + RB genes and INHIBIT them

Question 22

Cells of the ________________ are invaded by HPV

Answer 22

basal layer of epidermis

Question 23

HPV infections can result in two outcomes - what are they?

Answer 23

Latent or Lytic

Question 24

Latent HPV phase can have ______ and last for ______

Answer 24

no Sx

Last from Months to Years

Question 25

After HPV latency, what begins?

Answer 25

Production of : * DNA * Capsid * Particles

Question 26

Expression of structural proteins and vegetative DNA synthesis is limited to

Answer 26

terminally differentiated epidermal cells

Question 27

Morphologic changes of host cells from HPV infection is called \_\_\_\_\_\_

Answer 27

Koilocytosis

Question 28

HPV viremia lasts how long?

Answer 28

HPV does NOT cause viremia!!

Question 29

HPV proteins for virus assembly and release

Answer 29

L1, L2, E4

Question 30

HPV proteins for cell proliferation and replication?

Answer 30

E1 - E7 (\*but not E3)

Question 31

Koilocyte appearance

Answer 31

Multinucleated, Big, hyperchromatic nucleus with irregular outlines Perinuclear halo

Question 32

HPV transmitted on \_\_\_\_\_

Answer 32

fomites

Question 33

\_\_\_\_\_\_ may promote HPV transmission

Answer 33

asymptomatic shedding

Question 34

Lab tests for HPV can detect \_\_\_, \_\_\_, or \_\_\_\_

Answer 34

virus Nucleic Acid Viral Antigens Koiloctyotic cells

Question 35

HPV control

Answer 35

* Podophyllin resin, podofilox lotion * Aldara cream (= immune response modifier, stimulate cytokines and interferon) * VACCINE

Question 36

HPV vaccines

Answer 36

Gardasil and Cervarix

Question 37

Gardasil contains recombinant \_\_\_\_\_\_\_\_\_\_\_\_

Answer 37

VLP's of **6, 11, 16, 18**

Question 38

Guardasil protects against ____ strains of HPV, which cause 70% of Cervical neoplasias worldwide

Answer 38

16 + 18

Question 39

Gardasil mechanism

Answer 39

Triggers antibody response that prevents infection from the included types

Question 40

Gardasil dosing schedule

Answer 40

given in 3 doses ## Footnote **0, 2, 6 months**

Question 41

Polyomaviruses (viruses)

Answer 41

**BK + JC** | (SV40 [simian] and murine)

Question 42

Polyomaviruses differences from papillomaviruses

Answer 42

smaller and less complex replicate in: * Resp * urinary * CNS (JC only)

Question 43

Polyomaviruses are activated in _______ patients

Answer 43

immunocompromised (also pregnant patients, but no transmission to the fetus)

Question 44

Two infections caused by BK virus:

Answer 44

1. **Ureteral stenosis** (renal transplant pts) 2. **Hemorrhagic cystits** (bone marrow transplant pts)

Question 45

Main JC virus infection

Answer 45

**Progressive Multifocal Leukoencephalopathy** (PML) \*occurs in IC patients (e.g. AIDS)

Question 46

JC virus infects _____ cells, which results in ....

Answer 46

Oligodendrocytes multifocal demyelination of CNS

Question 47

PML prognosis

Answer 47

1-4 months

Question 48

Polyomaviruses are limited to _______ and \_\_\_\_\_\_\_

Answer 48

specific hosts + cell types

Question 49

JC+BK enter the ________ and infect \_\_\_\_\_\_\_\_ Then enter the _______ with minimal effect

Answer 49

respiratory tract lymphocytes kidney

Question 50

BK virus establishes _______ in kidney

Answer 50

latent infection

Question 51

JC cirus establishes infection where?

Answer 51

kidneys, B cells, monocyte-lineage cells

Question 52

After multiplication in the kidney, JC/BK cause ____________ , which either results in permanent latent kidney infection (in non-IC) or reactivation and progression (in IC pts).

Answer 52

causes transient secondary viremia

Question 53

BK more likely to cause _____ infections, JC may cause _____ infection

Answer 53

BK --\> UTI JC --\> CNS

Question 54

Polyomaviruses control/treatment

Answer 54

None