B1 cell biology Flashcards

(58 cards)

1
Q

Animal cell

A

Nucleus
Cell membrane
Cytoplasm

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2
Q

Plant cell

A
Nucleus 
Cell membrane 
Cytoplasm
Vacuole 
Chloroplasts 
Cell wall
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3
Q

Nucleus

A

Controls activities of cell
Contains DNA
DNA organised into chromosomes

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4
Q

Cell membrane

A

Controls passage of substances into and out of the cell

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5
Q

Cytoplasm

A

Is where most chemical reactions take place. (Aerobic Respiration)

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6
Q

Vacuole

A

Surrounded by a membrane and fluid filled
Fluid is called cell sap
Vacuoles are permanent structures in plants

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7
Q

Chloroplasts

A

Found in plant cells above ground

Contain chlorophyll that absorbs the light the plant needs for photosynthesis

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8
Q

Cell wall

A

Is an additional layer outside the cell membrane
Is made from cellulose fibres
Contains fibres that provide strength
Doesn’t regulate what enters or leaves the cell

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9
Q

Cell size

A

0.01-0.10 mm

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10
Q

Total magnification

A

Total magnification= mag of eyepiece x mag of object lens

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11
Q

Magnification of image

A

Mag of image=size of image/size of real object

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12
Q

Resolving power/resolution

A

Ability to distinguish 2 points

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13
Q

Mitochondria

A

Where aerobic respiration takes place. It has a double membrane although the internal membrane is folded.

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14
Q

Ribosomes

A

Tiny structures where protein synthesis takes place. They either lie free in the cytoplasm or be attached to an internal network of channels within the cytoplasm

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15
Q

TEMs

A

X1000000

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16
Q

SEMs

A

Reveal shape of structures

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17
Q

Electron microscope

A

Colour images

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18
Q

Required practical- using a light microscope to observe and record animal and plant cells

A

Mount live cells on a slide using a drop of water
Cover with a glass coverslip and avoid air bubbles
Start looking at it on low power and then swap to high power for more detail
Sketch the cell you saw, label and identify it as animal or plant

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19
Q

Eukaryotic

A

Have cell membrane, cytoplasm containing sub-cellular structures called organelles and a nucleus containing DNA

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20
Q

Prokaryotic

A
Ribosomes
Cytoplasm
Plasmids- small ring of DNA 
Cell membrane 
Cell wall (may be surrounded by a capsule) 
Flagellum 
Single loop of DNA not enclosed in a nucleus 
Smaller cells
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21
Q

Classification domains

A

Bacteria
Archaea
Eukaryota (animals,fungi,plants,protists)

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22
Q

Archaeans

A

Live in extreme environments eg.hot springs and salt lakes
Ribosomes are similar size and structure of bacteria.
Nucleic acid is closer to eukaryotes
2/3 of DNA are unfamiliar

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23
Q

How many chromosomes do we have

A

46 and 23 pairs

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24
Q

Mitosis

A

DNA molecule in nucleus
DNA replicates to form a double chromosome each half has an identical set of genes
DNA condenses to form a recognisable chromosome. The DNA is now compacted into 1/50000 of it original length

25
The cell cycle
1. The cell grows. No. Of sub cellular structures increases 2. DNA replicates to form 2 copies of each chromosome 3. Further growth and cell checked for errors 4. Mitosis- the chromosomes move apart and 2 nuclei form 5. Cytoplasm divides to make 2 identical cells 6. Temporary cell resting period, doesn't divide
26
Differentiation
As cells divide, new cells acquire certain features for their specific function. A cells shape,size and internal structure are adapted for its role. Most animal cells differentiate at an early stage
27
Sperm cell
``` Head Acrosome- contains enzymes to penetrate the egg Nucleus- 23 chromosomes Mitochondria- in spiral for energy Tail for movement Cell membrane 55 nano metres long 3 nano metres wide ```
28
Nerve cell
``` Extensions that communicate with other nerve cells Nucleus Fatty covering- the myelin sheath Main part of cell(cell body) Gap I'm myelin sheath- the nerve impulses jumps from one gap to the next making it quicker. Branches that connect with a muscle Motor nerve cell length 1m diameter 1-20 nano metres ```
29
Muscle cell
Mitochondria- provide energy for muscle contraction Protein filaments- slide over each other to produce muscle contraction The cells that make up a skeletal muscle fibre physically join together during their development
30
Protein filaments
Give the cells of a heart and skeletal muscle a striped appearance. In smooth muscle, found in the circulatory system, there are fewer filaments, which are thinner and less well organised
31
Cells, tissues and organs
Cell Tissue - lots of cells Organs- tissue grouped together Organ system- organs working together
32
What is cancer?
A damaged cell that divides uncontrollably.
33
Benign tumour
Slow growing Often have capsule around so easy to remove Not cancerous and rarely spread Can press on other body organs and look unsightly
34
Malignant tumour
Grow faster Can spread to other body tissues As it grows, cancer cells detach and can form secondary tumours in other parts of the body.
35
What triggers cancer?
Chemicals and other agents that cause cancer called carcinogens They damage the DNA causing mutations If it isn't discover in the cell cycle it continues to spread.
36
Mutations
Viruses Chemicals in the home,industry or environment Ionising radiation Ultraviolet radiation Lifestyle choices eg. Alcohol, smoking, diet
37
Stem cells
Unspecialised cells that can produce many different types of cells
38
How stem cells could help
Injuries eg. Spinal injuries leading to paralysis Conditions in which the body cells degenerate eg. Alzheimer's disease, diabetes and multiple sclerosis Cancers, or following treatments for cancer such a chemotherapy or radiation eg. Leukaemia Produce red blood cells
39
Embryonic stem cells
Removed from a living human embryo Religion doesn't agree with this as life starts at contraception Law says embryos can be created purely for scientific research
40
Rejection of stem cell transplants
If a stem cell is taken from a bank to which other people have contributed the patients body may reject it. A current solution is to find a close match between donor and patient. Or give drugs to suppress Immune system. A possible source is blood left in the umbilical cord and placenta after baby is born. It is easy to collect and store.
41
Therapeutic cloning
Producing stem cells with the same genes as the patient. So it wouldn't be rejected. Requires nuclear transfer.
42
Nuclear transfer
``` Human egg has nucleus removed and combined with the nucleus of the patient. Cell is simulated to divide Embryo produced is grown. After 4-5 days stem cells are removed Stem cells from the embryo are cultured ```
43
Scientific, ethical and social questions of stem cells
How successful would it need? After 60 divisions mutations have been observed Virus could transfer with stem cell leading to infection Is it morally right to create an embryo with the intent of destroying it Could an embryo simple be a resource for researchers Are there more positives than negatives Does it give patients false hope
44
Cells
Protein molecule- helically coiled nucleic acid molecule with protein coat. Virus- has lipoprotein envelope around it.
45
Mitosis occurs when...
Cells die or become damaged A eukaryotic organism reproduces by asexual reproduction Cancer cells divide Eukaryotic cells are cloned
46
Organisms need energy to...
Drive chemical reactions needed to keep them alive, including building large molecules For movement ( make our muscles contract) For keeping warm Cell division Maintain constant environment Active transport Transmit nerve impulses
47
Aerobic respiration
Uses glucose to create heat (ectothermic reaction) Glucose+oxygen}carbon dioxide + water C6H12O6+6O2}6CO2+6H2O Starts in cytoplasm then in mitochondria Respiration with oxygen
48
Anaerobic respiration
Respiration without oxygen In cytoplasm of cell Anaerobic respiration in yeast cells and other micro organisms is called fermentation
49
Fermentation
Glucose} ethanol+carbon dioxide | Anaerobic isn't as efficient a as aerobic
50
Oxygen debt
Glucose}lactic acid Incomplete oxidisation cases a build up of Latin acid and oxygen debt. The acid is taken to the liver by the blood where it is oxidised to co2 and waterproof converted to glucose then glycogen. Glycogen levels in the muscles and liver are then restored Needs oxygen to repay debt
51
Culturing bacteria
Best to grow them in large numbers to study them. Bacteria grow in culture, in or on a culture medium This is either a liquid nutrient broth or a gel agar
52
How to stop bacteria being infected
All equipment must be sterilised The inoculating loop must be sterilised by passing it through a Bunsen flame before and after use The lid of the agar plate must be secured, but not sealed using adhesive tape Agar plates must be sterilised in an autoclave before disposal This is an aseptic technique
53
Binary fission
Prokaryotes divide into 2 rapidly to form a colony
54
Bacteria growth curves
Lag phase- no reproduction. Bacteria copying DNA and proteins within their cell Exponential growth phase Stationary phase- resources become scarce so bacteria dies at same rate produced Death phase- bacteria poisoned by build up of toxins in the culture
55
Antibiotic testing
Disc diffusion technique Agar plate inoculated with bacterium being tested (spread over plate evenly) A disc of antibiotic is placed on the plate Different concentrations are put on plate as well
56
Lab vs school
Lab- mueller-Hinton agar with blood For human pathogens 37 degrees Celsius School- normal agar plate Bacteria (don't want to grow pathogens) 25 degrees Celsius
57
Validity of testing Antibiotics
A sample bacteria for testing must be representative of the population of bacteria Clear zones will be uniformly circular Measurements made with ruler or callipers If there is poor growth becomes anomalies
58
Required practical- investigating disinfectants
Agar plate inoculated with bacteria with different disks with different disinfectants and a control Inoculate at 25 degrees Celsius See which has the biggest clear zone as it is most effective for bacerierium staphylococcus aureus in hospitals