B1.1.3 - Light Microscopy Flashcards Preview

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Flashcards in B1.1.3 - Light Microscopy Deck (18):
1

Who was the first Scientist to see cells?

Scientist Robert Hooke

2

When did Robert Hooke first see cells?

350 years ago

3

How does a light microscope work?

It passes light through an object placed on a slide on the stage, and then through the 2 glass lenses.

4

What are the 2 different glass lenses called?

Objective lens and eyepiece lens

5

What are the 2 knobs called?

Course focus knob
Fine focus knob

6

Which glass lens do you look through with your eye?

Eyepiece lens

7

When first viewing cells, what position should the stage be at?

Lowest position

8

When first viewing, which objective lens should you choose?

Lowest magnification lens

9

What is total magnification?

Eyepiece lens magnification * Objective lens magnification

10

Why would you stain cells?

Because most are colourless and cells are easier to observe with stains

11

What stain would you use to view the nucleus of an animal cell?

Methylene blue

12

What stain would you use to view plant cell nuclei?

Iodine

13

What stains bacterial cell walls?

Crystal violet

14

How would you apply a stain?

Place the cells on a glass slide
Add 1 drop of stain
Place a coverslip on top
Tap on it lightly with a pencil to remove air bubbles

15

Why would you use a plastic coverslip instead of a glass coverslip?

The glass coverslip may shatter under the pressure of the pencil

16

What is the maximum resolution for the light microscope?

0.2 micro meters

17

What are the advantages to light microscope?

They can view alive specimens
They can view in colour
They are cheap
Easy to operate
Portable

18

What are the disadvantages of light microscopes?

They can’t view cells in as much detail as electron microscopes