Flashcards in B1.1.3 - Light Microscopy Deck (18):
Who was the first Scientist to see cells?
Scientist Robert Hooke
When did Robert Hooke first see cells?
350 years ago
How does a light microscope work?
It passes light through an object placed on a slide on the stage, and then through the 2 glass lenses.
What are the 2 different glass lenses called?
Objective lens and eyepiece lens
What are the 2 knobs called?
Course focus knob
Fine focus knob
Which glass lens do you look through with your eye?
When first viewing cells, what position should the stage be at?
When first viewing, which objective lens should you choose?
Lowest magnification lens
What is total magnification?
Eyepiece lens magnification * Objective lens magnification
Why would you stain cells?
Because most are colourless and cells are easier to observe with stains
What stain would you use to view the nucleus of an animal cell?
What stain would you use to view plant cell nuclei?
What stains bacterial cell walls?
How would you apply a stain?
Place the cells on a glass slide
Add 1 drop of stain
Place a coverslip on top
Tap on it lightly with a pencil to remove air bubbles
Why would you use a plastic coverslip instead of a glass coverslip?
The glass coverslip may shatter under the pressure of the pencil
What is the maximum resolution for the light microscope?
0.2 micro meters
What are the advantages to light microscope?
They can view alive specimens
They can view in colour
They are cheap
Easy to operate