Bacillus, Trueperella, Listeria Flashcards

1
Q

Listeria monocytogenes

A

Causative agent of human listeriosis
Agent of septicemic disease affecting rabbits and GPs
Most important in animals (birds and humans)

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2
Q

L. monocytogenes characteristics

A

Gram + intracellular bacteria, motile, non-spore forming, facultative anaerobe, rod shaped
Catalase and L- rammnose + and oxidase -
Resistant to NaCl

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3
Q

Beta hemolysin

A

L. monocytogenes expresses it
Causes RBC destruction

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4
Q

L. monocytogenes serotypes

A

Serotyped according to somatic (O) and flagellar (H) AGs
More than 14

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5
Q

Listeriosis

A

Sickens pregnant women and newborns, adults >65, immunocompromised
Facial and trigeminal n. Damage (facial paralysis and salivation)

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6
Q

Listeriosis food safety alert (CDC)

A

NY and PA found in Ice Cream House brand (Ice cream cups)
Enoki mushrooms
Deli meat and cheese

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7
Q

Listeriosis transmission

A

Eating contaminated food
Person to person (sexual contact)
Mother to child (pregnancy or childbirth)

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8
Q

Where has L. monocytogenes isolated from?

A

Meat or milk of goats, sheep, cattle, pig, chicken, quail, partridge, ostrich, buffaloes, fish
Veggies, seafoods

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9
Q

How long does listeriosis survive?

A

@ 5C survives for 13 (milk), 16 (brain), 12 (feces and silage) years

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10
Q

Listeria illness

A

Invasive: everywhere
Noninvasive: intestine
Symptoms start within 2 weeks after eating food

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11
Q

Listeria symptoms

A

Headache, stiff neck, confusion, meningitis, loss of balance, convulsions in pregnant women
Abortion, stillbirth, premature birth, septicemia

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12
Q

Circling disease (sheep)

A

Septicemic (horses and pigs), encephalitic (circling), abortion form
Causes encephalitis, repeat breeding endometriosis and abortion
Conjunctivitis in ruminants, mastitis in cattle

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13
Q

Listeriolysin O

A

Most significant virulence factor
Responsible for B-hemolysis on erythrocytes and destruction of phagocytic cells

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14
Q

Internalins (inIA)

A

Membrane proteins promoting cellular uptake
Virulence factor for Listeria

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15
Q

Listeriolysin (LLO)

A

Mediates intracellular survival and escape from phagosomes
Virulence factor for Listeria

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16
Q

Phospholipases

A

Involved in lateral spread to adjacent cells
Virulence factor for Listeria

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17
Q

L. innocua

A

Rare and cause meningoencephalitis in sheep (circling)
Acute and frequently fatal

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18
Q

L. ivanovii

A

Causes abortion in sheep and goats
Late abortion after 7 months in cattle and 12 weeks in sheep

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19
Q

Listeriosis diagnosis

A

Bacterial culture
Isolation from food, water, milk soil, silage and fecal samples
Oxford or RAPID’L.mono agar

20
Q

Listeriosis treatments for animals

A

Procaine penicillin G and oxytetracycline (early)

21
Q

Listeriosis treatments for humans

A

Penicillin, ampicillin, amoxicillin fluid therapy and supportive care

22
Q

Bacillus characteristics

A

Gram +, spore forming, aerobic or facultative anaerobes
Inhabitant of soil and water
Catalase +

23
Q

2001 Anthrax attacks

A

Letters with anthrax spores mailed to media office and senators
Killed 5 people and 17 infected

24
Q

Which bacillus species is non motile?

A

B. Anthracis (endospore)

25
Q

B. Anthracis

A

Causitive agent of anthrax- most important pathogen
Zoonotic and biothreat agent

26
Q

B. cereus

A

Human and canine food poisoning and rare case of bovine mastitis

27
Q

B. thuringiensis

A

Naturally occurring soil bacterium
Lepidopteran insect pathogen

28
Q

B. subtilis

A

Model organism
Food spoiler causing ropiness in bread and related food
Produce and secrete abx

29
Q

B. coagulans

A

Food spoilage of highly acidic, tomato-based products

30
Q

B. Anthracis growth

A

Colonies grow on common media
Not killed by heat fixation smear
Resistant ins soil (50 years)

31
Q

How is B. Anthracis killed?

A

Autoclaving 121C/15 min or dry heat
Not susceptible to phenolic, alcoholic and quaternary ammonium disinfectants

32
Q

Anthrax spread

A

Disease of cattle and sheep
Infected discharge large # of bacilli from mouth, nose and rectum
Biological weapon (spores)

33
Q

Anthrax source of infection

A

Bacilli sporulate in soil
Biting flies and consumption of infected meat

34
Q

Anthrax animals infection

A

Dead, bloated, without rigor mortis
Blood at body orifices
Staggering, trembling, breathing difficult, convulsions

35
Q

B. Anthracis virulence factors

A

Edema factor, protective Ag, lethal factor
Harmless individually, deadly together

36
Q

Protective Ag (PA)

A

Binds to the cell surface and forms a channel in the cell membrane to allow the other factors to enter the cell

37
Q

Anthrax in horses

A

Colic and diarrhea
Edema in throat or intestine causing asphyxiation
Septicemic in horses

38
Q

Anthrax in swine

A

Localization in pharyngeal tissues
Cutaneous form called bull nose

39
Q

Anthrax diagnose

A

Blood aspirated from superficial orifices, aqueous humor bloody discharges, spleen
Exam
Gram stain and McFadyen’s methylene blue, PCR
Ascoli prescription test

40
Q

Anthrax control and prevention in humans

A

Penicillin and oxytetracycline
Ciprofloxacin and doxycycline (prevention)
Vx- Biothrax

41
Q

Antitoxin use

A

Anthrax spores enter the body and activated → multiply, spread, and produce toxins → illness
Antitoxin treats in toxins ⬆️⬆️

42
Q

Anthrax spore vaccine

A

Sterne strain used in the form of live-spore vx
Used in all domestic animals 1 cc SQ

43
Q

Trueperella pyogenes

A

Faculative anaerobic, non-motile, non-spore forming, catalase +
Causes abscesses, mastitis, pneumonia in pigs and ruminants

44
Q

T. pyogenes importance

A

Opportunistic and economically important livestock: dairy and beef cattle and swine

45
Q

T. pyogenes infection

A

Physical or microbial trauma to the mucous membrane in udder, urogenital and upper resp. tract
Lesions in elbow and shoulder joints and abscesses in pigs
Cavities in lung tissue (chronic)
SC compression
Inflammatory and degenerative lesions of mammary glands

46
Q

T. pyogenes treatment

A

Beta-lactams: penicillin and cephalosporin and tetracyclines, macrolides