Bacteria - stain Flashcards
(64 cards)
1
Q
Staphylococcus
A
G+ cocci
2
Q
Strep
A
G+ cocci
3
Q
Moraxella catarrhalis
A
G- cocci
4
Q
Neisseria
A
G-cocci
5
Q
Bacillus
A
G+ rod
6
Q
Serratia
A
G-rod
7
Q
Shigella
A
G-rod
8
Q
mycobacterium
A
g+ rod (AF)
9
Q
enterobacter
A
g-rod
10
Q
clostridium
A
g+rod
11
Q
e coli
A
g- rod
12
Q
campylobacter
A
g-rod
13
Q
listeria
A
g+rod
14
Q
Lactobacillus
A
g+rod
15
Q
klebsiella
A
g-rod
16
Q
proteus
A
g-rod
17
Q
pseudomonas
A
g-rod
18
Q
gardnerella
A
g+ but variable
19
Q
salmonella
A
g-rod
20
Q
vibrio
A
g-rod
21
Q
corynebacterium
A
g+rod
22
Q
propionibacterium
A
g+rod
23
Q
yersinia
A
g-rod
24
Q
bacteroides
A
g-rod
25
helicobacter
g-rod
26
bordatella
g-rod
27
haemophillus
g-rod
28
actinomyces
branching filamentous
29
legionella
g-rod - (primarily intracellular - AF)
30
mycoplasma
no cell wall no gram stain -
31
nocardia
branching filamentous - AF
32
Treponema
spirochete
| dark field microscopy and fluorescent ab stain
33
Chlamydiae
pleomorphic (giemsa) - intracellular (lacks peptidoglycan)
34
leptospira
spirochete
35
bartonella
g-rod
36
borrelia
spirochete (giemsa)
37
rickettsiae
pleomorphic (giemsa) - intracellular
38
pasteurella
gram - rod
39
francicella
gram - rod
40
brucella
g- rod
41
ureaplasma
no cell wall
42
GRAM STAIN LIMITED
```
The microbes may lack real color
treponema
mycoplasma
mycobacteria
legionella
rickettsia
chlamydia
```
43
GIEMSA
```
Certain bugs really try my patients
Chlamydia
Borrelia
Rickettsia
Trypanosomes
Plasmodium
```
44
PAS (periodic acid schiff)
Tropheryma whipplei
45
Ziehl Neelsen (carbol fuchsin) - AF
Nocardia
Mycobacteria
Cryptosporidium - AIDs oocysts
46
India Ink
Cryptococcus neoformans
47
Mucicarmine
Cryptococcus - stains red
48
Silver stain
pneumocystis
legionella
h. pylori
49
```
Chocolate agar
Factors V (NAD+) and X(hematin)
```
H Influenzea culture
50
Thayer Martin agar
+ vanco (g+)
+ colistin (g-)
+ nystatin (fungi)
N gonorrhea culture
51
Thayer Martin agar
+ vanco
+ colistin
+ nystatin
N. meningitidis culture
52
Bordet-Gengou agar
Regan-Lowe medium
+ potato
+ charcoal, blood, antibiotic
Bordatella purtussis culture
53
Tellurite agar, Loffler medium
C. Diphtheria culture
54
Lowenstein-Jensen agar
M. tuberculosis culture
55
Eaton agar
| + cholesterol
M. pneumoniae culture
56
MacConkey agar
Lactose fermenting enterics (CULTURE)
57
slow LACTOSE FERMENTING enterics
citrobacter
serratia
others
58
fast Lactose fermenting enterics
```
E Coli (indole +)
Enterobacter (indole -)
Klebsiella
```
59
Eosin-methylene blue (EMB) agar
E Coli culture
60
Charcoal yeast extract
| cysteine + iron
Legionella culture
61
Sabourd agar
fungi culture
62
Classic aerobes (4)
Nagging pests must breath
nocardia
pseudamonas
mycobacterium
63
Classic anaerobes (4)
```
Frankly can't breath air
Fusobacterium
Clostridium
Bacterioides
Actinomyces
```
64
Aminoglycosides are ineffective against anaerobes
| why?
because they require O2 to activate
