Bacterial Growth Requirements and Gram Staining Flashcards

1
Q

What ingredient converts liquid to solid medium?

A

Agar

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2
Q

Medium that inhibits growth of certain organisms and permits growth of others.

A

Selective Media

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3
Q

Medium that gives colonies of a particular organism a distinct appearance (makes them all look different).

A

Differential Media

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4
Q

Medium that contains complexed materials that certain fastidious organisms require to grow.

A

Enrichment Media

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5
Q

Gram staining: Primary stain (applied first)

A

Crystal Violet

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6
Q

Gram staining: Mordant

A

Iodine

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7
Q

Gram staining: Decolorizer

A

Acetone/Alcohol

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8
Q

Gram staining: Counter Stain

A

Safranin

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9
Q

What color is Gram Stain?

A

Purple–resist decolorization and keep the primary stain

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10
Q

What color is Gram Negative?

A

Red–Easily decolorized - take up counter stain

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11
Q

Bacteria that cannot grow in the presence of free oxygen (cannot tolerate).

A

Anaerobe

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12
Q

Basically an aerobe but can grow in the absence of free oxygen (survive a wide range of conditions - hard to kill off)

A

Facultative Anaerobe

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13
Q

Can grow only in the complete absence of free oxygen (very difficult to grow - fussy)

A

Obligate Anaerobe

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14
Q

What pH is best for bacterial growth?

A

pH 7 - can tolerate a narrow range from 6 - 8

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15
Q

What is the best time to use bacterial cultures for lab studies?

A

18 - 24 hours

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16
Q

Toxins that are released when the cell is destroyed, because they are bound to the cell wall.

A

Endotoxins

Gram Negative

17
Q

Extremely delicate, difficult to culture, and involving specialized growth requirements.

A

Fastidious

18
Q

The interval of time between the invasion by a pathogenic microorganism and the appearance of first symptom of disease.

A

Incubation period

19
Q

The natural ability of an organism to remain unaffected by harmful substances in its environment.

A

Resistance

20
Q

Material spread on a slide for microscopic examination.

A

Smear

21
Q

The process of inculating a culture to provide for the growth of colonies on the surface of a solid medium. Streaking is accomplished by skimming a wire inoculating loop that contains the specimen across the surface of the medium using a back/forth motion.

A

Streaking

22
Q

An exotoxin produced by beta-hemolytic streptococci, which completely hemolyzes red blood cells.

A

Streptolysin

23
Q

Easily affected, lacking resistance.

A

Susceptible

24
Q

capable of of growing in absence of organic compounds; self-nourishing

A

autotrophic

25
Q

virus that attacks bacteria

A

bacteriophage

26
Q

organism living on decaying/dead organic matter

A

saprophyte

27
Q

relative power of a pathogen to cause disease

A

virulence