bacterial smear preparation and simple stain Flashcards
(61 cards)
This is done when basic stain is applied
Simple staining
What type of stain do we use in simple staining?
Basic stain
These helps us identify the bacteria under the microscope after applying staining reagent to the prepared smear
- Size
- Shape
- Arrangement
It is done after air drying the bacteria in the glass slide in smear preparation
Heat Fix
What electric charge do stain carries which the surface and cytoplasm of bacteria attracts?
Positive electric charge
What electric charge do the surface or cytoplasm of bacteria carries which stain attracts?
Negative electric charge
Two types of sample in the
DIRECT SMEAR
- from SWABS
- Concentrated centrifuged specimens
What do we do to avoid destruction of cellular entities in direct smear preparations when using swab?
Rolling the swab across the slide
In preparing concentrated centrifuged specimens in a glass slide, what do we use to transfer the sample?
Plastic transfer pipette
Needs to be sterile
Three types of samples for SMEARS
- Culture smears (subculture smears)
- Blood culture bottles
- Solid media
How many slide do we use in preparing smears from blood culture bottles?
One slide only
It is used to puncture the top of the blood culture bottle
Safety venting subculture unit
It is used for placing a small drop of water in the squares of glass slides
(not sure with this question)
I-mL tuberculin syringe
with the needle removed
These are added first before smearing bacteria to the glass slide
- Sterile NSS
- Distilled water (H20)
Why do we rub the colony into the glass slide with a drop of H20 or distilled water in it?
for emulsification
or to emulsify
What should be the appearance of the glass slide after emulsifying it?
Turbid
Two types of culture
- Pure culture
- Mixed culture
Three types of tool used for implanting the bacteria into the glass slide
- Inoculating loop
- Inoculating needle
- Disposable Inoculating loop and needle
Two sizes for inoculating loop
- 10uL
- 1uL
It is commonly reffered to the use of a single stain to color a bacterial organism
Simple staining
How many stain do we use in simple staining?
1 stain only
Three common types of stains in simple staining
- Methylene blue
- Basic fuchsin
- Crystal violet.
Why do we use stain to color bacterial organism?
It is useful in determining basic morphology and the presence or absence of certain kinds of granules.
How many seconds/minutes is done in simple staining?
30 seconds to 2 minutes
depending on the affinity of the dye
