Optochin
Differentiates what organism? How?
aka P-Disk
Strep pneumoniae are SENSITIVE
Oxidase positive GNRs (list 6)
Vibrio Aeromonas Neisseria Pseudomonas Campylobacter Pasteurella
List tests (4) which differentiated β hemolytic Strep
Bacitracin
•GAS SENS
•GBS RES
TMP-SXT
•GAS RES
•GBS RES
PYR
•GAS+
Hippurate
•GBS+
List tests (3) to diff Group D Strep
Bile esculin
•GDE+
•S. bovis+
6.5 NaCl
•GDE RES
•S. bovis SENS
PYR
•GDE+
•S. bovis-
DNAse
Distinguishes Moraxella catarrhalis from Neisseria
Who needs X and V factors?
Haemophilus spp
What is X and V factor?
V = NAD
X = hemin
Superoxol test
30% H2O2
(Catalase is 3%)
N gonorrhea brisk bubbling
N meningitidis weak delayed bubbles
Neisseria CHO use profiles
Glucose ==> gonorrhea
Maltose ==> meningitidis
Sucrose
Lactose ==> lactamica
Red ==> Yellow (acid)
Moraxella is negative for all
What does the Staph streak test grow?
Haemophilus spp
String test
V cholera vs non-cholera Vibrio
Stuart’s broth
Christensens broth
Urease test
Rapid splitters = Proteus
Slow splitters = Klebsiella
Who are PYR+?
GAS
GDE
