GNRs Flashcards

(31 cards)

1
Q

List the four hallmarks of Enterobacteriaceae

A

Nitrate+
Oxidase-
Glucose ferm+
Catalase+

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2
Q

List anaerobic GNRs:

A

Bacteroides
Porphyromonas
Prevotella
Fusobacteria

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3
Q

MacConkey-GROWTH, Glucose-POS, Nitrate-POS, Oxidase-NEG, Catalase-POS

A

Enterobacteriaceae

Proteus
Shigella
Citrobacter
Edwardsiella
Escherichia
Salmonella
Klebsiella
Serratia
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4
Q

MacConkey-GROWTH, Glucose-POS, Oxidase-POS

A

Vibrio
Aeromonas
Plesiomonas

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5
Q

MacConkey-GROWTH, Glucose-NEG

A

Pseudomonas **
Xanthomonas maltophilia
Acinetobacter
Flavobacterium **

*** oxidase-POS

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6
Q

Fastidius organisms

A
Francisella
Brucella
Bordetella
Pasteurella
Legionella
Campylobacter
Capnocytophaga
Streptobacilus monoliformis
Haemophilis
Helicobacter
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7
Q

List members of the Proteae family:

List a uniformly POS biochemical test in these bitches:

A

Proteus
Morganella
Providencia

**Phenylalanine deaminase

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8
Q

Shigella:

How many organisms does it take to fuck you up?

What lab characteristic is memorable about Shigella?

A

100-200 organisms

**It’s NEG for everything… except METHYL RED

Therefore… lactose? Nope
H2S gas? Nope
VP? Nope
Indole? Citrate? PDA? Nope, nope, nope.

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9
Q

Yersinia entercolitica’s name to fame?

**List three species:

A

MCC of blood transfusion-related sepsis

Remember YCEP*

    • enterocolitica
    • pseudotuberculosis
    • pestis
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10
Q

What other enteric rod does Citrobacter resemble?

How do they differ?

A

Salmonella
*both citrate-POS

Citrobacter is POS in the ONPG reaction (“O-NitroPhenyl-B-D-Galactopyranoside”)

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11
Q

What other enteric rod does Edwardsiella resemble?

How do they differ?

*Any disease associations?

A

Salmonella
**both produce H2S

Edwardsiella is Indole-POS

  • Iron overload
  • co-infection with E. histolytica
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12
Q

Escherichia:

Key BIOCHEM features (2)

A

Lactose-FERM

Indole-POS like Edwardsiella

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13
Q

List types of E. coli infection:

A

ETEC = Enterotoxic = Traveler’s diarrhea
heat-stable vs. guanylate/heat-labile vs. adenylate cyclase

EIEC = Enteroinvasive = Dysentery

EPEC = Enteropathogenic = gastroenteritis in infants of poo’ countries

EAEC = Enteroaggregative = infant diarrhea in poo’ countries

EHEC = Enterhemorrhagic** gets its own flashcard

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14
Q

Which E. coli is O157:H7?

Its biochem characteristic?

A

EHEC

Sorbitol NEG

  • plate on MacConkey-sorbital vs. MacConkey-lactate
  • clear vs. pink = presumptive EHEC!

Miscellaneous shit:
Verotoxin (Shiga-like) = inhibits protein synth and damages enterocytes

Toxin in blood = endothelial damage

HUS

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15
Q

Which Salmonella species cause bacteremia?

MoA (mode of action):

A

S. typhi, paratyphi, and cholerasuis

Invade bowel wall and chill in reticuloendothelial cells of liver, spleen, and gb ==> reinfection

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16
Q

I say Klebsiella, you say… (biochem test)

Disease assoc? (List two)

A

Voges-Proskauer!

Pneumonia in alcoholics (K. pneumniae)
Rhinoscleroma (K. rhinoscleromatis)

17
Q

Which Enterobacteriaceae are non-motile?

A

Klebsiella
Shigella

**Yersinia at 37 C

18
Q

What is prodigiousum?

The microbe that produces this is known for what?

A

A red pigment produced by some strains of Serratia

Nosocomial infections from medical devices

19
Q

What else is H2S-producing? (Besides Salmonella)

A

CEP

Citrobacter
Edwardsiella
Proteus

20
Q

Escherichia’s key BIOCHEM features (2)

A

Lactose-FERM

Indole-POS like Edwardsiella

21
Q

What else is VP-pos? (Besides Klebsiella)

A

SHE

Serratia
Hafnia
Enterobacter

22
Q

Which Enterobacteriaceae ferment lactose?

A

KEE

Klebsiella
Escherichia
Enterobacter

23
Q

Interpret this TSI/KIA:

slant/deep
K/K

A

Not Enterobacteria!

PCEM (pchem?)

Pseudomonas
Campylobacter
Eikenella
Moraxella

24
Q

Interpret this TSI/KIA:

slant/deep
K/A

A

Glucose fermented only

SSPY

Shigella
Serratia
Providencia
Yersinia

25
Interpret this TSI/KIA: | slant/deep K/A +H2S
Glucose fermented only + H2S-producing SPEC Salmonella Proteus Edwardsiella Citrobacter
26
Interpret this TSI/KIA: (slant/deep) A/A What if it produces gas too (list 2 bugs)
Lactose is their bitch Escherichia Klebsiella Enterobacter (The KEEs! Remember, these are the three lactose fermenters)
27
Media for Vibrio? Vibrio cholerae vs. parahemolyticus
Thiosulfate citrate bile salt agar V. cholera: Sucrose+
28
TCBS agar: what's the differentiating factor?
Sucrose V. cholera will be positive (YELLA)
29
Wtf is El Tor biotype?
V. cholera with WEAK string test, has B-hemolysis, and agglutinates on chicken RBCs
30
How is V. cholera classified?
(1) O Ags (>150) (2) Biotype (class vs. El Tor) (3) Serotype (Ogawa, Inaba, Hikojima) * O1 causes most cases * non-O1, nobody gives a fuck * O139 causes cholera in India
31
What's the point of sucrose in TSI?
To rule out Salmonella/Shigella (neither ferment sucrose) A/A result rules them out