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Flashcards in GNRs Deck (31)
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1

List the four hallmarks of Enterobacteriaceae

Nitrate+
Oxidase-
Glucose ferm+
Catalase+

2

List anaerobic GNRs:

Bacteroides
Porphyromonas
Prevotella
Fusobacteria

3

MacConkey-GROWTH, Glucose-POS, Nitrate-POS, Oxidase-NEG, Catalase-POS

Enterobacteriaceae

Proteus
Shigella
Citrobacter
Edwardsiella
Escherichia
Salmonella
Klebsiella
Serratia

4

MacConkey-GROWTH, Glucose-POS, Oxidase-POS

Vibrio
Aeromonas
Plesiomonas

5

MacConkey-GROWTH, Glucose-NEG

Pseudomonas ***
Xanthomonas maltophilia
Acinetobacter
Flavobacterium ***

*** oxidase-POS

6

Fastidius organisms

Francisella
Brucella
Bordetella
Pasteurella
Legionella
Campylobacter
Capnocytophaga
Streptobacilus monoliformis
Haemophilis
Helicobacter

7

List members of the Proteae family:

List a uniformly POS biochemical test in these bitches:

Proteus
Morganella
Providencia

**Phenylalanine deaminase

8

Shigella:

How many organisms does it take to fuck you up?

What lab characteristic is memorable about Shigella?

100-200 organisms

**It's NEG for everything... except METHYL RED

Therefore... lactose? Nope
H2S gas? Nope
VP? Nope
Indole? Citrate? PDA? Nope, nope, nope.

9

Yersinia entercolitica's name to fame?

**List three species:

MCC of blood transfusion-related sepsis

Remember *Y*C*E*P*

** enterocolitica
** pseudotuberculosis
** pestis

10

What other enteric rod does Citrobacter resemble?

How do they differ?

Salmonella
*both citrate-POS

Citrobacter is POS in the ONPG reaction ("O-NitroPhenyl-B-D-Galactopyranoside")

11

What other enteric rod does Edwardsiella resemble?

How do they differ?

*Any disease associations?

Salmonella
**both produce H2S

Edwardsiella is Indole-POS

*Iron overload
*co-infection with E. histolytica

12

Escherichia:

Key BIOCHEM features (2)

Lactose-FERM
Indole-POS *like Edwardsiella*

13

List types of E. coli infection:

ETEC = Enterotoxic = Traveler's diarrhea
heat-stable vs. guanylate/heat-labile vs. adenylate cyclase

EIEC = Enteroinvasive = Dysentery

EPEC = Enteropathogenic = gastroenteritis in infants of poo' countries

EAEC = Enteroaggregative = infant diarrhea in poo' countries

EHEC = Enterhemorrhagic** gets its own flashcard

14

Which E. coli is O157:H7?

Its biochem characteristic?

EHEC

Sorbitol NEG

*plate on MacConkey-sorbital vs. MacConkey-lactate
*clear vs. pink = presumptive EHEC!

Miscellaneous shit:
Verotoxin (Shiga-like) = inhibits protein synth and damages enterocytes

Toxin in blood = endothelial damage

HUS

15

Which Salmonella species cause bacteremia?

MoA (mode of action):

S. typhi, paratyphi, and cholerasuis

Invade bowel wall and chill in reticuloendothelial cells of liver, spleen, and gb ==> reinfection

16

I say Klebsiella, you say... (biochem test)

Disease assoc? (List two)

Voges-Proskauer!

Pneumonia in alcoholics (K. pneumniae)
Rhinoscleroma (K. rhinoscleromatis)

17

Which Enterobacteriaceae are non-motile?

Klebsiella
Shigella

**Yersinia at 37 C

18

What is prodigiousum?

The microbe that produces this is known for what?

A red pigment produced by some strains of Serratia

Nosocomial infections from medical devices

19

What else is H2S-producing? (Besides Salmonella)

CEP

Citrobacter
Edwardsiella
Proteus

20

Escherichia's key BIOCHEM features (2)

Lactose-FERM
Indole-POS *like Edwardsiella*

21

What else is VP-pos? (Besides Klebsiella)

SHE

Serratia
Hafnia
Enterobacter

22

Which Enterobacteriaceae ferment lactose?

KEE

Klebsiella
Escherichia
Enterobacter

23

Interpret this TSI/KIA:

(slant/deep)
K/K

Not Enterobacteria!

PCEM (pchem?)

Pseudomonas
Campylobacter
Eikenella
Moraxella

24

Interpret this TSI/KIA:

(slant/deep)
K/A

Glucose fermented only

SSPY

Shigella
Serratia
Providencia
Yersinia

25

Interpret this TSI/KIA:

(slant/deep)
K/A +H2S

Glucose fermented only + H2S-producing

SPEC

Salmonella
Proteus
Edwardsiella
Citrobacter

26

Interpret this TSI/KIA:

(slant/deep)
A/A

What if it produces gas too (list 2 bugs)

Lactose is their bitch

Escherichia

Klebsiella
Enterobacter

(The KEEs! Remember, these are the three lactose fermenters)

27

Media for Vibrio?

Vibrio cholerae vs. parahemolyticus

Thiosulfate citrate bile salt agar

V. cholera: Sucrose+

28

TCBS agar: what's the differentiating factor?

Sucrose

V. cholera will be positive (YELLA)

29

Wtf is El Tor biotype?

V. cholera with WEAK string test, has B-hemolysis, and agglutinates on chicken RBCs

30

How is V. cholera classified?

(1) O Ags (>150)
(2) Biotype (class vs. El Tor)
(3) Serotype (Ogawa, Inaba, Hikojima)

*O1 causes most cases

*non-O1, nobody gives a fuck

*O139 causes cholera in India