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Flashcards in Basics of Immunology Deck (127)
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1

PAMPs

Pathogen-associated molecular patterns. On the intruder,. What is recongnized by PRRs on the cell surface of innate immune cells. These are proteins that humans don't have, so we know they don't belong (ie a protein of bacterial cell well or dsRNA)

2

PRRs

Pattern-recognition receptor. Proteins on the cell surface expressed by cells of innate immune system. PRRs identify the PAMPs expressed on foreign intruders

3

TLRs

Toll-like receptors. A type of PRR. There are at least 10 TLRs and are located where infections enter, ie lining blood vessels

4

DAMPs

Damage-associated molecular pattern. Stress or damage indicators expressed by body cells (ie paintball example)

5

What are some foreign patters recognized by TLRs?

TLR1: Lipopeptide (bacterial)
TLR2: Zymosan (fungal)
TLR3: dsRNA (viral)
TLR4: endotoxin
TLR5: flagellin (bacteria)
TLR6: Lipopeptide (mycoplasma)
TLR7: ssRNA (Influenza)
TLR8: ssRNA (viral)
TLR9: Unmethylated CpG (herpes)
TLR10: asthma connection

6

What is the final transcription factor that is most commonly activated by inflammation?

Bad bug makes endotoxin >> endotoxin is recognized by TLR >> Signal cascade >> Activates NF-KB >> Inflammation

7

Cytokines

Made by PAMP-stimulated cells. Small cell-signaling protein molecules that are secreted by the immune system for intercellular communication Helps inflammation

8

Chemokine

Made by PAMP-stimulated cells. Family of small cytokines. Induce directed chemotaxis of WBC to site of injury.

9

Innate to Adaptive Immune Response

At the wound site, immature DCs get activated by the cytokines and chemokines secreted by the innate immune response, and they take up anything they can find. Activated (mature) DCs leave the local wound and travel to the nearest lymph node. They "show" the antigen they have eaten to the lymphocytes, and the adaptive immune response develops with T cells, B cells and DCs.

10

Dendritic Cells

Phagocytic cells at the interfaces between the body and the world (skin, lung, mucous membranes...). Immature DCs are the best phagocyte ever! Mature DCs are the best antigen presenting cell ever! (A change in phenotype occurs when the DC matures)

11

T Lymphocytes

Start development in bone marrow, but mature in THYMUS. (Helper) T lymphocytes recognize and remove foreign substances. Two main classes: Helper T cells and Killer T cells.

12

The epic journey of Helper T Cells

They recognize antigens using surface receptors which see antigens presented by the DC that is traveling via lymphatics. When the HTC recognizes the foreign material it becomes activated and proliferates. The daughter cells travel through the body until they reach where the antigen first invaded. There they are re-stimulated and release short-range mediators called lymphokines. These mediators call up much of the inflammatory response, attracting monocytes/macrophages

13

Killer T Cells

Kill any body cell that they identify as containing abnormal molecules. Examine surface of incoming DCs, but they are also looking for fragments on MHC Class I antigen presenter, which are on ALL cells. Appropriate clones of Killer T cells proliferate and daughter cells circulate. When a daughter cell binds a cell showing the same peptide it delivers a lethal "hit" signaling the target cell to commit suicide.

14

The role of B cells in immunity

Recognize and remove foreign substances and protect extracellular spaces of the body by releasing antibodies into tissue fluids, blood, and bodily secretions. They arrange for phagocytosis and recognize antigens via surface receptors and become activated and proliferate. They do NOT require recognition of MHC. Fully differentiated B cells are called plasma cells and are antibody producing "factories"

15

What are the 5 immunoglobulin classes?

IgG, IgM, IgD, IgA, IgE

16

IgG

most abundant antibody, 2 adjacent IgG molecules binding an antigen cooperate to activate COMPLEMENT. It is the only antibody that passes from mother to fetus. Phagocytic cells have receptors for the Fc of bound IgG>Opsonizing

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Complement

A system of proteins that enhances inflammation and pathogen destruction

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IgM

Even better at activating COMPLEMENT than IgG. First antibody to appear in blood after exposure to new antigen. Replaced by IgG in 1-2 weeks. It is the ONLY antibody made in the fetus.

19

IgD

Antibody inserted into B cell membranes as their antigen receptor

20

IgA

Most important antibody in secretions (tears, saliva, breast milk). Associated with SECRETORY COMPONANT which makes the antibody resistant to digestive enzymes and plays a role as the first line of defense against microorganisms

21

IgE

Resistance to parasites and worms. Fc attaches to mast cells in tissues. Once attached, when it encounters and antigen it will cause the mast cell to make protaglandins, leukotrienes and cytokines and release its granules which contain mediators of inflammation (ie histamine). Also triggers Mast Cells to release Eosinophils to combat parasites

22

Type I immunopathology

Immediate hypersensitivity. Patient makes too much IgE to environmental antigen

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Type II Immunopathology

Autoimmunity due to antibodies that react against self. Treat diseases with immunosuppressives

24

Type III Immunopathology

Patient makes antibody against a soluble antigen. Antigen-antibody complex sometimes gets trapped in basement membrane of capillaries, where they activate Complement causing inflammation. (ie Systemic Lupus Erythemtosus and Rheumatoid Arthritis) Also, large doses of penicillin can cause Type III

25

Type IV

T-cell mediated. Can be autoimmune or innocent bystander disease. Example: in TB most of the cavity formation in lungs is T cell mediated, not bacterium mediated

26

AIDS

HIV infection of TH cells; binds to CD4 molecules they have on their surface. Uses reverse transcriptase to insert itself into the cells genome, where it remains latent until T cell is activated, leading to loss of TH cells

27

Chronic frustrated immune response

Antigen is not to "self", but something you can't get rid of, like gut bacteria or gluten

28

Leukocytes

Nucleated cells of the blood, white blood cells. The buffy coat when you centrefuge blood

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Mononuclear cells

Leukocytes whose nucleus has a smooth outline; monocytes and lymphocytes

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Polymorphonuclear cells

Cells whose nucleus is lobulated, also called granulocytes because they have prominent cytoplasmic granules; Eosinophils, Basophils and Neutrophils