Flashcards in BB Ch 4 121-134 Deck (53)
Give the Order and Family of the laboratory rat.
Which rat strain is a model for mononuclear cell leukemia?
Which rat strain is a model for juvenile insulin-dependent diabetes mellitus?
Name the rat strain that is a model for multiple sclerosis and various experimentally induced autoimmune diseases.
Which rat strain is an obesity model?
The rat strain that is a host for transplantable Morris hepatoma and serves as a model of spontaneous autoimmune thyroiditis.
Which rat strain is a model for spontaneous hypertension?
SHR (Spontaneous Hypertensive Rat)
Which rat strain is a model of myeloma and production of IgG autoantibody?
Which rat strain serves as a model for congenital genitourinary anomalies and prostatic adenocarcinomas?
Which rat strain serves as a model for inducible, transplantable myeloid leukemia, hydronephrosis, and bladder carcinoma?
BN (Brown Norway)
Which rat strain's primary use is as a prostatic adenocarcinoma model?
Which rat strain serves as the inbred rat model for National Toxicology Program's Carcinogen Bioassay Program and the National Institute on Aging?
F-344 (Fischer 344)
Describe important characteristics of the Brattleboro rat strain.
Diabetes insipidus (autosomal recessive)
Describe important characteristics of the Gunn rat strain.
Jaudice, kernicterus (autosomal recessive). Kernicterus is a rare neurological condition that occurs in some newborns with severe jaundice.
Describe important characteristics of the Nude rat strain.
T cell deficient (autosomal recessive)
Describe important characteristics of the Obese SHR.
Type 4 hyperlipoproteinemia (autosomal recessive)
What is the appropriate temperature and humidity for a rat housing area?
20-26C or 68-79F, 30-70% humidity (Guide 8th ed.)
How often should solid bottom rat cages be sanitized?
Name the gland that is associated with chromatodacryorrhea in the rat.
Which pair of salivary glands of the rat is composed entirely of mucous secreting cells?
T or F. Rats have a dividing ridge in their stomach that is an anatomical barrier to vomiting.
True - the ridge is called the limiting ridge or margo plicatus
What is the lobe pattern of the rat lung?
A) 1 right, 4 left
B) 4 right, 1 left
C) 2 right, 4 left
D) 4 left, 2 right
B - 4 right, 1 left (just like the mouse)
T or F. The pulmonary vein of the rat has cardiac striated muscle within its wall and bronchoconstriction is controlled by vagal tone.
Which of the following is not a characteristic of the rat.
A) Cowper’s glands
B) A bicornuate uterus with 2 cervices
C) A unipapillate kidney
D) A foramen of Magendie
D - rats have no Foramen of Magendie, which drains CSF from the 4th ventricle to the cisterna magna
What type of diet is used most commonly for toxicological and Good Laboratory Practices studies?
A) Certified natural ingredient diet
B) Purified diet
C) Chemically defined diet
D) Closed formula diet
A - GLP studies require that natural ingredient diets be certified to be free of contaminants that can influence study results
At what age does puberty occur in rats?
What is the gestation length of rats?
At what age do the eyes of rat pups open?
14-17d, but the retina is not fully developed until 30-40d
At what age are rat pups typically weaned?
Usually 20-21d, but can be as early as 17d
What is the length of the rat estrus cycle?
What two drugs can be used to synchronize estrus in rats?
Synchronization can be accomplished by giving 40mg methoxyprogesterone in the water for 6d, the injection of 1 IU PMS IM
What is the earliest day at which pregnancy can be accurately palpated in rats?
T/F. Newborn rats are hairless, blind, have short tails and open ear canals.
F - the ear canals are closed at birth
T/F: Rat pups are altricial at birth
True - altricial refers to the need for young to be fed and taken care of for a long duration
T/F: Rat pups are not nidiculous at birth
False - Nidicolous animals are those that stay at their nest or birthplace for a long time after birth, due to their dependence on the parents for feeding, protection and learning survival skills.
Genus and species of the Norway rat
Genus and species of the Black rat
Genus and species of the common lab rat
Rattus norvegicus - this was an albino orginally selected by hobbyists
What level of illumination is recommended for rats to prevent phototoxic retinopathy?
130-325 lux (Guide 8th ed.)
T/F: Rats have multipapillate kidneys.
False - Rats have unipapillate kidneys.
Name the three general classification of diets.
1) Natural ingredient: made with agricultural products & by products; composition varies b/w batches (unless certified), most common diet in research
2) Purified: defined ingredient list, often more expensive and less palatable
3) Chemically defined: very basically defined ingredient list (specific amino acids, carbohydrates, triglycerides, etc); very expensive and less palatable
T/F: Persistence of the vaginal plate is a rare cause of infertility in rats.
False - the vaginal plate is congenital epithelium that closes the vagina and is usually gone by 40-80d of age; persistence is a common cause of infertility
When does estrus begin in the rat?
A) Before puberty
B) At the same time as puberty
C) After puberty
A) Estrus begins before puberty, which is defined as the ability to bear viable young. Puberty in most rat strains/stocks occurs at 2-3 months of age.
Describe the technique for sex determination of pre-implantation embryos.
HY antigen, which is identical to anti-mullerian hormone, is produced by the testis (Sertoli cells) and prevents the formation of the Mullerian ducts, which develop into the uterus. 80% of rat embryos that develop in the presence of anti-HY antibody will become female after implantation.
What condition can develop in relation to the urinary system if rat pups are weaned before 17d of age?
Urinary obstruction - maternal stimulation is required for spontaneous urination prior to 17d of age.
Name three way of inducing pseudopregnancy in rats.
1) Vasectomized males
2) Mechanical vaginal stimulation
3) Electrical cervical stimulation
T/F: Brown Norway and F-344 rats are generally more docile than Sprague-Dawley and Lewis rats.
False - SD & Lewis rats are more docile
For what model are inbred Wistar Ottawa Karlzburg W (WOKW) rats?
Metabolic syndrome & obesity
Name two types of breeding schemes in the rat?
Continuous monogamous mating and intermittent monogamous mating.
Name the structure that allows the for the collection of blood from medial aspect of the orbit.
Orbital venous sinus
Name the accessory sex glands of male rats.
Bulbourethral (Cowper's) glands, prostate, seminal vesicles
T/F: Rats have a bicornuate uterus with two distinct ossa uteri and cervices.