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beetroot CSM practical Flashcards

(11 cards)

1
Q
A
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2
Q

detailed method

A
  • use extract and water to prepare a series of 6 test tubes containing 5cm3 of different concentrations of extract. concentrations should be equally spaced and cover a range from pure water to pure extract
  • label the tubes 0,2,4,6,8,10
  • with a second set of test tubes add 2cm3 of 100% alcohol to a test tube and put a bung in the tube.
  • label the tube with alcohol concentration
  • repeat for all different alcohol concentrations
  • put tubes of alcohol in the water bath until temperature of alcohol reaches 30 degrees
  • blot 10 discs of beetroot with a paper towel to remove excess water
  • gently put the beetroot in each test tube, replace bungs straight after
  • leave tubes in water bath for 5 mins. shake them gently every minute or so. then take them out
  • immediately pour each solution into a clean test tube, labelled appropriately
  • use colorimeter to measure absorbance and colour.
  • make a calibration curve graph
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3
Q

risk assessment

A

safety issues with ethanol- no naked flames, ensure good ventilation and wear eye protection

care using cork borers and scalpels

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4
Q

why is it important to wash the beetroot discs

A

to wash off any excess pigment and that pigment released was from the effect of alcohol

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5
Q

describe a suitable control for the investigation

A

distilled water and beetroot, to show that the alcohol was causing the leakage of pigment / to compare it with the effects of the alcohol

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6
Q

why shake the tubes every minute

A

distribution of pigment, increase contact of all surfaces with alchol

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7
Q

why pour the solution immediately from the test tube into the clean one

A

to limit variation in time discs are in alcohol- make sure all discs were in contact with alcohol at the same time and so no more pigment can diffuse out of the discs

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8
Q

why would the results be different if we used cooked beetroot

A

cooking would damage the plasma membranes so denatures proteins and less pigment present in tissue

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9
Q

why would a decrease of 5 degrees affect the results

A

less kinetic energy
molecules move slower
lower rate of diffusion
membranes less fluid

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10
Q

why did students who carried out the same investigation find their results were different

A

variation in judging colour
different beetroot
different surface area of beetroot

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11
Q

an alternative way of carrying out this practical is to use detergent instead of alcohol. suggest why detergent would affect the cells in a similar way to ethanol
explain why the detergent used needs to be colourless

A
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