{ "@context": "https://schema.org", "@type": "Organization", "name": "Brainscape", "url": "https://www.brainscape.com/", "logo": "https://www.brainscape.com/pks/images/cms/public-views/shared/Brainscape-logo-c4e172b280b4616f7fda.svg", "sameAs": [ "https://www.facebook.com/Brainscape", "https://x.com/brainscape", "https://www.linkedin.com/company/brainscape", "https://www.instagram.com/brainscape/", "https://www.tiktok.com/@brainscapeu", "https://www.pinterest.com/brainscape/", "https://www.youtube.com/@BrainscapeNY" ], "contactPoint": { "@type": "ContactPoint", "telephone": "(929) 334-4005", "contactType": "customer service", "availableLanguage": ["English"] }, "founder": { "@type": "Person", "name": "Andrew Cohen" }, "description": "Brainscape’s spaced repetition system is proven to DOUBLE learning results! Find, make, and study flashcards online or in our mobile app. Serious learners only.", "address": { "@type": "PostalAddress", "streetAddress": "159 W 25th St, Ste 517", "addressLocality": "New York", "addressRegion": "NY", "postalCode": "10001", "addressCountry": "USA" } }

mitosis practical Flashcards

(8 cards)

1
Q

detailed method

A
  • cut off root tip off onion/garlic and add to beaker of 10ml hydrochloric acid
  • leave in for 10 mins
  • rinse the root tip in distilled water
  • place specimen on microscope slide
  • cover specimen with toluidene bleu stain and macerate with mounted needle to separate cells
  • add cover slip and spread material gently
  • look through microscope
  • chromosomes should stain dark blue
  • take a photo of sample
  • calculate mitotic index
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

why are you told to observe cells that are found at or just behind the root tip called the meristem

A

most ideal area to look for mitosis, meristem cells are undifferentiated, and constantly dividing, making them ideal to observe.
cells in meristem are totipotent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

explain why cells may appear elongated with no visible chromosomes if they are taken too far along the root

A

in the region of cell elongation, they are increasing in size but not dividing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

why use HCL

A

-HCL quickly denatured any protein including enzymes
- it quickly stops cellular processes
- kills cells halting the process immediately so the cells in different stages can be seen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

why use toluidine blue

A

Dye is used for staining of acid rich tissues
As it can be absorbed by nucleic acid
- it increases contrast
- makes chromosomes visible

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

why is it necessary to spread the material on the slide

A

cells could overlap each other
stain might not have been dispersed properly

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

why is it suggested to look at several fields of view

A

-comparison
-mean of mitotic index

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what does diploid number of 16 mean

A

-8 chromosomes from male
-8 from female

How well did you know this?
1
Not at all
2
3
4
5
Perfectly