Benzodiazepines analgesia and cognition

Question 1

_____ ______ supressess the sensitised response to heat and mechanical stimulation evident in rodent models of both inflammatory and neuropathic pain

Answer 1

intrathecal diazepam

Question 2

intrathecal diazepam supresses the sensitised response to ____ and ______ stimulation evident in rodent models of both inflammatory and neuropathic pain

Answer 2

heat and mechanical stimulation

Question 3

intrathecal diazepam suppresses the sensitised response to heat and mechanical stimulation evident in rodent models of both _______ and ______ pain

Answer 3

inflammatory and neuropathic

Question 4

MEASURING ANALGESIA IN MOUSE MODELS OF INFLAMMATORY AND NEUROPATHIC PAIN
measuring analgesia in mouse models of inflammatory pain what was used and where

Answer 4

zymosan A injection into left hind paw

Question 5

MEASURING ANALGESIA IN MOUSE MODELS OF INFLAMMATORY AND NEUROPATHIC PAIN
measuring analgesia in mouse models of neuropathic pain what was used

Answer 5

tie off the sciatic nerve

Question 6

MEASURING ANALGESIA IN MOUSE MODELS OF INFLAMMATORY AND NEUROPATHIC PAIN
how is heat hyperalgesia measured

Answer 6

paw withdrawal latency upon exposure to a defined radiant heat

Question 7

MEASURING ANALGESIA IN MOUSE MODELS OF INFLAMMATORY AND NEUROPATHIC PAIN
how is mechanical sensitization measured

Answer 7

paw withdrawal with von frey filaments (varying the site of filament)

Question 8

MEASURING ANALGESIA IN MOUSE MODELS OF INFLAMMATORY AND NEUROPATHIC PAIN
how was cold allodynia measured

Answer 8

time spent lifting, shaking, or licking the paw after a drop of acetone on the injured paw

Question 9

the analgesic effects of intrathecal diazepam in mouse models of inflammatory and neuropathic pain are primarily mediated by what GABAa receptors

Answer 9

alpha2/3-GABAa receptors

Question 10

where are alpha2 GABAaRs densely expressed

Answer 10

lamina II/II dorsal horn neurons
and in primary afferent dorsal root ganglion neurons

Question 11

intrathecal diazepam suppresses the sensitised response to heat and mechanical stimulation of both inflammatory and neuropathic pain
these effects of diazepam are maintained in the ________ mouse but NOT the ________ mouse
fill the blank
alpha1H101R
alpha2H101R-GABAaR mouse

Answer 11

response to heat and mechanical stimulation of both heat and mechanical stimulation in models of both inflammatory and neuropathic pain. These effects if diazepam ARE maintain in the alpha1H101R mouse but NOT the alpha2H101R-GABAaR mouse

Question 12

in rats L-838,417 suppresses/enhances sensitization of mechanical and heat evident in both inflammatory and neuropathic mouse models

Answer 12

suppresses

Question 13

DEVELOPING ALPHA5-NAMs AS COGNITIVE ENHANCERS
diazepam/flunitrazepam impair cognition, particularly when combined with what

Answer 13

alcohol

Question 14

DEVELOPING ALPHA5-NAMs AS COGNITIVE ENHANCERS
however, NAMs of the alpha5betagamma2 subtype, that bind to the BZ site between the alpha5-gamma2 subunit interface exhibit pro-cognitive properties in what mouse models:

Answer 14

huntingtons
alzheimers
down’s syndrome

Question 15

what are two ways to assess cognition in mice

Answer 15

morris water maze
novel object recognition task

Question 16

impaired LTP is enhanced by what

Answer 16

alpha5 NAMs of mouse models of huntingtons and alzheimers disease

Question 17

alpha5 GABAaRs are expressed where

Answer 17

extrasynaptically in the hippocampal CA1 neurons

Question 18

alpha5 GABAaRs are expressed extrasynaptically in hippocampal CA1 neurons wher they mediate a ____ current

Answer 18

tonic

Question 19

the alpha5 KO mouse exhibits improved performance in what

Answer 19

water maze spatial learning paradigm

Question 20

the cognitive effects impairing the effects of diazepam are _____ in the alpha5 KO mouse

Answer 20

absent

Question 21

NAMs of alpha5 GABAaRs improve cognitive performance in mouse models of down’s syndrome and huntingtons and _____ LTP in these mice

Answer 21

enhance

Question 22

alpha 5 GABAaRs NAMs acting on the benzodiazepine binding site, are being assessed in clinical trials for cognitive disorders, but also depression and stroke

Answer 22

just a statement soz