Bilirubin Auto Total FS

Question 1

Intended use:
(side note)

Diagnostic reagent for quantitative in vitro determination of total bilirubin in human serum or heparin plasma on automated
photometric systems.

Answer 1

Diagnostic reagent for quantitative in vitro determination of total
bilirubin in human serum or heparin plasma on automated
photometric systems.

Question 2

In the liver, bilirubin is
conjugated with __

Answer 2

glucoronic acid

Question 3

High levels of total bilirubin are
observed in 60 – 70% of neonates due to an increased postpartum
breakdown of erythrocytes and because of ___

Answer 3

delayed function of
enzymes for bilirubin degradation.

Question 4

Method used

Answer 4

Photometric test using 2,4-dichloroaniline (DCA)

Direct bilirubin in presence of diazotized 2,4-dichloroaniline forms a red colored azocompound in acidic solution. A specific mixture of detergents enables a safe determination of the total bilirubin [3].

Question 5

Reagents
(Components and Concentrations)

Answer 5

✅R1:
(1)…..Phosphate buffer
(50 mmol/L)

(2)…..NaCl — 150 mmol/L

✅R2:
(1)….. 2,4-Dichloroaniline
(5 mmol/L)

(2)…..HCl – 130 mmol/L

Question 6

Reagents are stable up to the date of expiry indicated on the kit, if
stored at____ and contamination is avoided.

Answer 6

2 – 8°C

Question 7

May be corrosive to metals.

Answer 7

H290

Question 8

Causes skin irritation

Answer 8

H315

Question 9

Causes serious eye
irritation

Answer 9

H319

Question 10

Keep only in original packaging

Answer 10

P234

Question 10

very toxic to aquatic life with long lasting
effects

Answer 10

H410

Question 11

Wash
hands and face thoroughly after handling

Answer 11

P264

Question 12

Wear protective gloves/protective
clothing/eye protection.

Answer 12

P280

Question 13

If eye irritation persists:
Get medical advice/attention

Answer 13

P337+P313

Question 14

Collect spillage.

Answer 14

P391

Question 15

If in eyes: Rinse cautiously with
water for several minutes. Remove contact lenses, if present
and easy to do.

Answer 15

P305+P351+P338

Question 16

If eye irritation
persists: Get medical advice/attention.

Answer 16

P337+P313

Question 17

Stability:

Answer 17

1 day at 20 – 25°C
7 days at 4 – 8°C
6 months at –20°C

Question 17

Absorb spillage
to prevent material damage.

Answer 17

P390

Question 18

Specimen

Answer 18

Human serum or heparin plasma

Question 19

Wavelength

Answer 19

545/658 nm

Question 20

Temperature

Answer 20

37°C

Question 21

Sample/Calibrator (??)
Reagent 1 (??)
Reagent 2 (??)

Answer 21

Sample/Calibrator ➡️ 2 µL
Reagent 1 ➡️ 80 µL
Reagent 2 ➡️ 20 µL

Question 22

Addition reagent 2

Answer 22

Cycle 19 (286 s)

Question 23

Calculation With calibrator

Answer 23

Bilirubin [mg/dL] = ΔA Sample/ΔA Cal (multiply) Conc. Cal [mg/dL]

Question 24

DiaSys TruCal U is recommended for calibration. TruCal U calibrator values have been made traceable to the______ reference material. Use DiaSys _____for internal quality control. Each laboratory should establish corrective action in case of deviations in control recovery.

Answer 24

✅NIST SRM 916 ✅TruLab N and P

Question 25

Side note: Measuring range up to ✅30 mg/dL. When values exceed this range, samples should be diluted ✅1 + 1 with NaCl solution ✅(9 g/L) and the result multiplied by ✅2

Answer 25

🩵

Question 26

(Performance Characteristics) Measuring range up to ___

Answer 26

30 mg/dL.

Question 27

Interfering substance Interferences Interferences ≤ 10% up to: ???

Answer 27

Ascorbic acid ➡️ 30 mg/dL Hemoglobin ➡️500 mg/dL Lipemia (triglycerides) ➡️1000 mg/dL

Question 28

Bilirubin Conversion Factor (mg/dL to µmol/L)

Answer 28

Bilirubin [mg/dL] x 17.1

Question 29

Reference Range in NEONATES

Answer 29

24 h ➡️ < 8.8 [mg/dL] ➡️ < 150[µmol/L] 2nd day ➡️ 1.3 – 11.3 [mg/dL] ➡️ 22 – 193 [µmol/L] 3rd day ➡️ 0.7 – 12.7 [mg/dL] ➡️ 12 – 217 [µmol/L] 4th – 6th day ➡️ 0.1 – 12.6 [mg/dL] ➡️ 1.7 – 216 [µmol/L]

Question 30

Reference Range in CHILDREN

Answer 30

>1 month ➡️ 0.2 – 1.0 [mg/dL] ➡️ 3.4 – 17

Question 31

Reference Range in ADULTS

Answer 31

➡️ 0.1 – 1.2 [mg/dL] ➡️ 1.7 – 21 [µmol/L]