Lab 2: Icterus Index by Meulengracht (Hilado, 1996)

Question 1

Icterus Index by Meulengracht principle

Answer 1

To find the find out the yellow intensity of the sample

The intensity of the golden yellow color imparted to the serum by bilirubin is measured by comparing the serum color with the color of standard solution of dichromate standard.

Question 2

standard used

Answer 2

Potassium dichromate standard

Question 3

side note

One unit of Icterus Index has been defined as the color of a 1:10,000 solution to potassium dichromate.

Answer 3

One unit of Icterus Index has been defined as the color of a 1:10,000 solution to potassium dichromate.

Question 4

1. Transfer ____ ml of unhemolyzed serum to a test tube and add ____ mL of phosphate buffer solution, pH 7.4

Answer 4

🩵1 mL - unhemolyzed serum

🩵9 mL - phosphate buffer solution

Question 5

3. If the reading is high, transfer to _____ mL e mixture to another test tube and dilute to ____with distilled water. Mix and read using the same wavelength.

Answer 5

🩵 1 mL
🩵 10 distilled water

Question 6

Read the working potassium dichromate standard setting the instrument at ____ against distilled water.

Answer 6

zero

Question 7

Calculation formula:

Answer 7

OD of unknown/OD of standard…. multiply by 10

Question 8

Notes:

\2. The patient should not eat food items which contain yellow pigment for ___to____hours prior to the drawing of blood for the test.

Answer 8

14 to 48 hours

Question 8

Notes:
1. It is essential that the serum used for the Icterus Index determination is absolutely free of visible hemolysis and chyle.

Answer 8

🩵

Question 9

(Normal Value)
how many units Icterus Index:

Answer 9

4-6 Units

Question 10

Preparation of Reagents:

Dissolve ___gram of potassium dichromate in ____ ml of distilled water.

Add 0.1 ml of concentrated sulfuric acid and dilute to 100 ml. Keep in brown bottle.

Answer 10

🩵1 gram of potassium dichromate

🩵90 ml distilled water

Question 11

Preparation of Reagents:

how many ____mL of concentrated sulfuric acid is added and dilute to ____mL .

And then keep it what bottle color?

Answer 11

🩵0.1 mL of sulfuric acid
🩵Dilute to 100 mL
🩵Brown bottle

Question 12

Standard Working Solution

Dilute ____ml of Stock Standard Solution to 1L with distilled water.

This makes a 1:10,000 solution of potassium dichromate.

Answer 12

🩵10 mL

Question 13

What determines intensity of the yellow color of this solution represents one Icterus Index Unit.

Answer 13

Standard Working Solution

Question 14

Phosphate Buffer Solution, pH 7.4

Into a flask, place____gram monopotassium dihydrogen phosphate.

Add ____ml distilled water and dissolve.

Answer 14

23.16 grams of monopotassium dihydrogen phosphate.

400 mL