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BIO CHEM 4 Flashcards

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1
Q

cDNA, a term used in recombinant DNA technology means

Competitive DNA
Chemical DNA
Complex DNA
Complementary DNA

A

Complementary DNA

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2
Q

The process of introduction of foreign DNA into an animal cell is called

Transversion
Conversion
Inversion
Transfection

A

Transfection

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3
Q

A segment of DNA that reads from the same forward and backward is called

Palindromic DNA
Complementary DNA
Plasmid DNA
Copy DNA

A

Palindromic DNA

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4
Q

Which of the following enzyme is used in PCR?

Taq DNA polymerase
HRP
EcoRI
EcoRII

A

Taq DNA polymerase

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5
Q

Which of the following is helpful in distinguishing DNA of one individual from another?

PCR
Reverse transcriptase
cDNA
RFLP

A

RFLP

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6
Q

Which of the following is the correct order of organization of genetic material from largest to smallest?

Genome, chromosome, gene, nucleotide
Nucleotide, gene, chromosome, genome
Gene, nucleotide, chromosome, genome
Chromosome, genome, nucleotide, gene

A

Genome, chromosome, gene, nucleotide

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7
Q

Which of the following codons is not a termination codon for protein synthesis?

UUU
UAG
UAA
UGA

A

UUU

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8
Q

Which of the following are the two methods of screening?

Hybridization and PCR
ELISA and blotting
ELISA and PCR
PCR and RFLP

A

Hybridization and PCR

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9
Q

Short sub-sequence of a cDNA sequence is

Expressed sequence tag
Sequence tagged site
cContig
YAC

A

Expressed sequence tag

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10
Q

Short DNA sequence having single occurrence in genome is

Expressed sequence tag
Sequence tagged site
Contig
YAC

A

Sequence tagged site

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11
Q

A set of overlapping DNA segments that together represent a consensus region of DNA is

Expressed sequence tag
Sequence tagged site
Contig
YAC

A

Contig

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12
Q

Which of the following statements is false?

BAC is a circular DNA molecule
YAC is a linear DNA molecule
Taql is a DNA polymerase
BAC is a linear DNA molecule

A

BAC is a linear DNA molecule

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13
Q

Which of the following is not denatured by heating steps during PCR?

DNA pol
RNA pol
Restriction enzymes
DNA ligase

A

DNA pol

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14
Q

Which of the following statements regarding DNA fingerprinting is false?

DNA fingerprinting cannot be used for paternity testing

DNA profile using STR (short terminal repeats) is unique to an individual

PCR is used for DNA profiling

Forensic analysis makes use of SNPs (single nucleotide polymorphisms) in coding sequences to distinguish between individuals

A

DNA fingerprinting cannot be used for paternity testing

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15
Q

How are expression vectors useful?

To produce protein products
Used for genomic libraries
Used for chromosome synthesis
Used for finger printing

A

To produce protein products

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16
Q

Who was the first person to develop DNA finger printing?

David Suzuki
Khorana
Alec Jaffreys
Gilbert

A

Alec Jaffreys

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17
Q

Southern blotting technique is used in

Monoclonal antibody production
In vitro culture
Genetic finger printing
Polymerase chain reaction

A

Genetic finger printing

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18
Q

Electrophoresis helps to separate

DNA segments
Cells from DNA
Tissues
RNA from DNA

A

DNA segments

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19
Q

During DNA profiling, DNA nucleotides hybridized with probe can be detected through

Electrophoresis
Polymerase chain reaction
Autoradiography
Hybridoma

A

Hybridoma

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20
Q

Which of the following term is associated with DNA finger printing?

Hybridoma
Site specific mutagenesis
Shotgun cloning
RFLP

A

RFLP

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21
Q

Recombinant DNA construction involves

Cleaving DNA with restriction endonuclease and joining with ligase

Cleaving DNA with ligase and joining with endonuclease

Cleaving and joining DNA with restriction endonuclease

Cleaving DNA with restriction endonuclease and joining with polymerase

A

Cleaving DNA with ligase and joining with endonuclease

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22
Q

The study of full complement of proteins expressed by a genome is called

Proteome
Proteomics
Genomics
Protein formation

A

Proteomics

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23
Q

The effects of protein on an entire organism is described in

Phenotypic function
Cellular function
Molecular function
Structural genomics

A

Phenotypic function

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24
Q

The network of interactions engaged in by protein at cellular level is described in

Phenotypic function
Cellular function
Molecular function
Structural genomics

A

Cellular function

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25
The precise biochemical activity of a protein is described in Phenotypic function Cellular function Molecular function Structural genomics
Molecular function
26
Sequencing of genomic DNA is included in Phenotypic function Cellular function Molecular function Structural genomics
Structural genomics
27
The goal of structural proteomics project is To crystallize and determine the structure of as many proteins as possible, in many cases with little or no existing information about protein function To identify and sequence of all the genes present in the human body To introduce new genes to human beings To remove disease causing genes from humans
To crystallize and determine the structure of as many proteins as possible, in many cases with little or no existing information about protein function
28
Conserved gene order can be termed as Ortholog Synteny Paralog Microarray
Synteny
29
Genes of different species but possessing a clear sequence and functional relationship to each other are Ortholog Synteny Paralog Microarray
Ortholog
30
Genes of same species, similarly related to each other are Ortholog Synteny Paralog Microarray
Paralog
31
Collection of microscopic DNA spots attached to solid surface are Ortholog Synteny Paralog Microarray
Microarray
32
Rauolfia serpentine, to save this plant under the threat of extinction, which of the following techniques is useful? Genetic engineering In vitro culture DNA fingerprinting Hybridoma technology
In vitro culture
33
Transgenic organisms are generally Extinct organisms Naturally occurring and endemic Produced by traditional plant breeding technique Produced by gene transfer technology
Produced by gene transfer technology
34
Dolly, the first animal produced by cloning is a Cow Sheep Rat Dog
Sheep
35
Gene therapy is a technique preferred to cure inherited diseases by Repairing the faulty gene Introducing the correct copy of the gene Adding new cells to the body PCR
Introducing the correct copy of the gene
36
Glucose is added to the tissue culture media as a/an Carbon source Growth regulator Solidifying agent Antibiotic
Carbon source
37
Explant can be a Cut part of the plant used in tissue culture Plant extract used in tissue culture Source of growth regulators added to media Solidifying agent
Cut part of the plant used in tissue culture
38
Meristem culture helps in developing which of the following? Tall plants Disease resistant plants Virus free plants Hybrid plants
Virus free plants
39
Which of the following is used to transfer genes in plants? Ti plasmid pBR 322 EcorI pUC 18
Ti plasmid
40
Which of the following bacterium is used for gene transfer in plants? Agrobacterium Azatobacter Rhizobium Ecoli
Agrobacterium
41
Production of a large number of genetically similar plants through plant tissue culture is called Recombinant DNA technology Gene therapy Micro propagation Hybridoma technology
Micro propagation
42
Which of the following is an essential fatty acid? Linolenic Palmitic Oleic Stearic
Linolenic
43
Which of the following is a polar derivative of cholesterol? Bile salt Oestrogen Vitamin D Progesterone
Bile salt
44
Which of the following fatty acid has the least melting point? Palmitic acid Stearic acid Arachidonic acid Timnodonic acid
Timnodonic acid
45
Out of the following which is not a source of glycerol? Adipolysis Glycolysis Glycogenolysis Diet
Glycogenolysis
46
Which of the following is false about fatty acids? Melting point of fatty acids decreases with increase in degree of saturation Lipids in tissues that are subjected to cooling are more unsaturated Naturally occurring unsaturated long-chain fatty acids are nearly Trans-configuration The membrane lipids contain mostly unsaturated fatty acids
Naturally occurring unsaturated long-chain fatty acids are nearly Trans-configuration
47
Which of the following fatty acid has 16 carbon atoms? Linolenic acid Oleic acid Palmitic acid Stearic acid
Palmitic acid
48
Which of the following is a hydroxyl fatty acid? Linoleic acid Palmitic acid Linolenic acid Cerebronic acid
Cerebronic acid
49
Out of the following, which is not an essential amino acid? Linolic acid Linolenic acid Arachidic acid Arachidonic acid
Arachidic acid
50
The number of milligrams of KOH required to neutralize the free and combined fatty acid in one gram of a given fat is called Saponification number Iodine number Acid number Polenske number
Saponification number
51
Which of the following is a storage form of lipid? Glycolipid Phospholipid Sufolipid Triacyl glycerol
Triacyl glycerol
52
Out of the following, cholesterol does not serve as a precursor for which compounds? Vitamin D Sex hormones Bile salts Bile pigments
Bile pigments
53
Which of the following is a sphingophospholipid? Lecithin Sphingomyelin Plasmolegen Cardiolipin
Sphingomyelin
54
Which of the following glycerophospholid acts as a lipotropic agent? Cardiolipin Phosphatidylserine Phosphatidylinositol Phosphatidylcholine
Phosphatidylcholine
55
Which of the following phospholipids is a component of inner mitochondrial membrane? Plasmologen Cephalin Lecithin Cardiolipin
Cardiolipin
56
In which of the following glycerophospholipids two phosphatidic acids share a single glycerol? Cardiolipin Phosphatidylserine Phosphatidylinositol Phosphatidylcholine
Cardiolipin
57
Platelet activating factor stimulates the release of which of the following compounds? Vasopressin Serotonin Adrenaline Cortisol
Serotonin
58
Which of the following group of membrane lipids predominate in plant cells? Galactolipids Sphingolipids Glycerophospholipids Archaebacterial ether lipids
Galactolipids
59
Which of the following membrane lipids have a direct glycosidic linkage between the head-group sugar and the backbone glycerol? Glycolipids Phospholipids Sphingolipids Ether lipids
Glycolipids
60
The backbone of phospholipids is L-glycerol 1-phosphate L-glycerol 3-phosphate D-glycerol 3-phosphate sn-glycerol 1-phosphate
L-glycerol 3-phosphate
61
What is the head-group alcohol in plasmolegen and platelet-activating factor? Alkene Choline Alkane Acetic acid
Choline
62
Which of the following does not contain glycerol? Glycerophospholipids Galactolipids Sphingolipids Triacylglycerols
Sphingolipids
63
Phosphorylation of phosphatidylinositol yields Phosphatidylinositol 4, 5-biphosphate Phosphatidylinositol 3, 5-biphosphate Phosphatidylinositol 3, 4-biphosphate Phosphatidylinositol 5, 6-biphosphate
Phosphatidylinositol 4, 5-biphosphate
64
Which of the following vitamin is derived from cholesterol? A B C D
D
65
What leads to the activation of protein kinase C? Release of intracellular Ca2+ + diacylglycerol Release of intracellular Mg+ + diacylglycerol Release of intracellular Ca2+ + glycerol Release of intracellular Ca2+ + triacylglycerol
Release of intracellular Ca2+ + diacylglycerol
66
Which of the following serves as a specific binding site for the proteins involved in membrane fusion during exocytosis? Phosphatidylinositol Phosphatidylinositol 4, 5-biphosphate Phosphatidylinositol 3, 5-biphosphate Phosphatidylinositol 3, 4-biphosphate
Phosphatidylinositol 4, 5-biphosphate
67
Which of the following hormone is responsible for the activation of phospholipase C? Serotonin Cortisol Vasopressin Adrenaline
Vasopressin
68
An example of glycerophospholipid involved in cell signaling is Cardiolipin Phosphatidic acid Phosphatidylcholine Phosphatidylinositol
Phosphatidylinositol
69
Which of the following type structure contains all the three glycosphingolipids? B structure A structure O structure AB structure
O structure
70
The lipids with potent bio activities derived from isoprenoid precursors are common in Vitamin A, K, ubiquinone and dolichol Vitamin A, D, ubiquinone and dolichol Vitamin A, B, D and K Vitamin A, B, K and dolichol
Vitamin A, K, ubiquinone and dolichol
71
Which of the following vitamin is responsible for Ca2+ and phosphate metabolism? A K E D
D
72
How many products are obtained by the hydrolysis of phosphatidylinositol by phospholipase C? 1 2 3 4
2
73
Which of the following is false about lipids? They are either strongly hydrophobic or amphipathic They are more soluble in water Extraction of lipids from tissues require organic solvents They are insoluble in water
They are more soluble in water
74
Which would move faster in thin layer chromatography? Beeswax Phosphatidylinositol Cholesterol Steroid
Beeswax
75
In which type of chromatography, solvents of increasing polarity are passed through a column of silica gel? High performance liquid chromatography Thin layer chromatography Adsorption chromatography Gas-liquid chromatography
Adsorption chromatography
76
Phosphatidylinositol, phosphatidylglycerol and phosphatidylserine are easily separated by Absorption chromatography TLC HPLC Gas-liquid chromatography
TLC
77
For the determination of fatty acid composition, transesterification is done in a warm aqueous solution of KCl+methanol KOH+methanol NaOH+methanol H2O+methanol
NaOH+methanol
78
A mixture of fatty acyl methyl esters are separated based on Charge Chain length and degree of saturation Molecular weight Ionic size
Chain length and degree of saturation
79
Which technique is preferred in the separation of fatty acyl methyl esters from a mixture? Gas-liquid chromatography Absorption chromatography TLC Centrifugation
Gas-liquid chromatography
80
The dye used in TLC for detecting separated lipids by spraying the plate is Mordant Alizarin Rhodamine Fuchsin
Rhodamine
81
Which of the following is not a phospholipase? A C D K
K
82
In which type of chromatography lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones? Absorption chromatography Thin layer chromatography Gas-liquid chromatography HPLC
Thin layer chromatography
83
Which of the following acts as a passive electrical insulator? Myelin sheath Plasma membrane Inner mitochondrial membrane Outer mitochondrial membrane
Myelin sheath
84
Which of the following membranes contain low cholesterol and high cardiolipin? Myelin sheath Plasma membrane Inner mitochondrial membrane of hepatocyte Cell wall
Inner mitochondrial membrane of hepatocyte
85
Which of the following is important in signal transduction triggered by hormones? Phosphatidylinositol and its derivatives Phosphatidylserine and its derivatives Phosphatidylglycerol and its derivatives Phosphatidylethanolamine
Phosphatidylinositol and its derivatives
86
Which of the following are the major component of chloroplast membrane of plants and are absent from animal cells? Glycolipids Phospholipids Ether lipids Triacylglycerols
Glycolipids
87
How many types of lipid aggregates are formed when amphipathic lipids are mixed with water? 1 2 3 4
3
88
Which of the following types of lipid aggregates has the maximum stability? Micelles Bilayer Liposome Myelin sheath
Liposome
89
When cross sectional area of the head group and acyl side chain is similar, which of the following lipid aggregates formation is favored? Micelles Bilayer Liposome Myelin sheath
Bilayer
90
When cross sectional area of the head group is greater than that of the acyl side chain, which of the following lipid aggregates formation is favored? Micelles Bilayer Liposome Myelin sheath
Micelles
91
Which of the following is a choline containing lipid? Sphingomyelin Phosphatidylserine Phosphatidylglycerol Phosphatidylethanolamine
Sphingomyelin
92
A platelet is able to play its role in the formation of blood clot when one of the glycerophospholipids in the plasma membrane moves into the outer leaflet Phosphatidylserine Phosphatidylethanolamine Phosphatidylinositol Phosphatidylglycerol
Phosphatidylserine
93
Which of the following glycerophospholipids is not common in inner leaflet (cytoplasmic)? Phosphatidylserine Phosphatidylethanolamine Phosphatidylinositol Phosphatidylcholine
Phosphatidylcholine
94
Which of the following has high lipid content? Myelin sheath Plasma membrane Inner mitochondrial membrane Outer mitochondrial membrane
Myelin sheath
95
Plasma membrane is permeable to ATP Glucose K+ Urea
Urea
96
How are the intrinsic proteins in cell membrane distributed? Uniform Random Symmetric Asymmetric
Asymmetric
97
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BIO CHEM (10 decks)

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