BIOCHEM LAB_ QUIZ 1 Flashcards

(62 cards)

1
Q

Organic molecules that serve as the building blocks of proteins

A

Amino Acids

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2
Q

Short chains of amino acids

A

Peptides

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3
Q

Longer and complex peptides

A

Polypeptides

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4
Q

Structure of Amino Acids

A

Amino Group - left
Side chain or R group - bottom
Carboxyl group - right
Hydrogen - up
Alpha carbon - center

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5
Q

Proton or hydrogen donor

A

Acid

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6
Q

Proton acceptor / accepts hydrogen

A

Base

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7
Q

Molecule that contains both positive and negative charge

A

Zwitterion form

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8
Q

Negative charge in zwitterion form

A

Carboxylate ion

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9
Q

Positive charge in zwitterion form

A

Ammonium ion

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10
Q

Process of formation of peptides

A

Condensation

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11
Q

Happens when the reactive amino group of an amino acid interacts with the reactive carboxylic group of another amino acid

A

Condensation

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12
Q

Librates water molecules

A

Condensation

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13
Q

2 amino acids

A

Dipeptide

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14
Q

3 amino acids

A

Tripeptide

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15
Q

50 amino acids or more

A

Proteins or polypeptides

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16
Q

Aka amphoteric

A

Acid-base property

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17
Q

Act as a base and an acid depending on the reactant involved in the chemical reaction

A

Amphoteric

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18
Q

Acts as a base when an acid is added in the system

A

Amino acid

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19
Q

Acts an an acid when a base is added in the system

A

Amino acids

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20
Q

Results to overall charge of POSITIVE because?

A

Because of the reactive ammonium ion

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21
Q

Donates 1 hydrogen ion to the OH group from the base and becomes an _________ group

A

Amino

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22
Q

Donates 1 hydrogen ion to the OH group from the base

A

Ammonium ion

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23
Q

This results to an overall charge of NEGATIVE because?

A

Coming from the carboxylate

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24
Q

There is a certain pH where is EVENLY BALANCED between 2 forms

A

Isoelectric point

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25
As the dipolar zwitterion with a net charge of ________
Zero
26
There is a negative and a positive pole
Dipolar
27
As the pH moves away from the isoelectric point, the amino acids may exist as __________ or an __________.
Cations and ion
28
As the pH become lower than the isoelectric point, it happens when acids is added in the system. The amino acids become _______ charge.
positively
29
As the pH become higher than the isoelectric point, it happens when acids is added in the system. The amino acids become _______ charge.
negatively
30
Four different functional groups are attached to it
Chiral carbon
31
four different functional group are attached to it
CHIRAL CARBON
32
because of this property, the alpha/chiral carbon is assymetric
STERIOCHEMISTRY
33
all of the functional groups are the same, but the location of the functional groups are mirror images but non-superimposable
ENANTIOMERS
34
amino acids can exist as enantiomers, they exhibit optical property known as
OPTICAL ACTIVITY
35
determined by the use of POLARIMETER
OPTICAL ACTIVITY
36
positive for dextrorotatory
clockwise
37
negative for levorotatory
counter clockwise
38
capacity to interact with water
polarity
39
hydrophobic
Non-Polar Amino Acids
40
water-fearing
non-polar amino acids
41
1 amino, 1 carboxylic, and a side chain that polar but neutral
polar neutral amino acids
42
1 amino acids, 2 carboxyl group
Polar Acidic Amino Acids
43
2 amino groups. 1 carboxyl group
Polar basic amino acid
44
Generally soluble in water
Amino acids
45
Insoluble in non-polar organic solvents
Amino acids
46
Crystal in solids with high melting point
Melting points
47
200-300 *c
Melting point
48
Tastes of amino acids
- sour or umami - tasteless (leu) - sweet (gly,ala,val) - bitter (arg, lle)
49
Sweet or bitter taste
L-amino acids
50
Sweet flavor
D-amino acids
51
Formed by acid-base neutralization reaction
Salt formation
52
When a base reacts with an amino acid
Formation of salt with alkis
53
Chemical reaction that takes place in metabolic processes that involve enzymes
Decarboxylation
54
Removal of a carboxyl group from an amino acid
Decarboxylation
55
This takes place when an amino acids contains 2 carboxyl groups in the structure
Reaction with Ammonia
56
-nH2
Amino group
57
Formation of salt with an acid
Salt formation
58
The reactive amino group reacts with ninhydrin to form Ruhemanns’ purple
Reaction with nynhydrin
59
Quantitative determination of amino acids & proteins
Ninhydrin test
60
Transfer of an amino group from an amino acid to a keto acid to form a new amino acid catalyzed
Transamination
61
Transfer of an amino group from an amino acid to a keto acid to form a new amino acid catalyzed
Transaminases
62
Provides NH3+ for urea synthesis
Oxidative deamination