MICROPARA_LAB Flashcards

(56 cards)

1
Q

A system of accurately ground
lenses arranged to give sharp,
clear, magnified images of
minute objects

A

MICROSCOPE

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2
Q

The technology of making very
small things visible to the
naked eye

A

MICROSCOPY

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3
Q

the process of enlarging something
only in appearance

A

MAGNIFICATION

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4
Q

related to scaling up visuals or
images to be able to see more detail

A

MAGNIFICATION

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5
Q

ability of the lenses to distinguish
fine detail and structure

A

RESOLVING POWER

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6
Q

ability to distinguish two points a
specified distance apart

A

RESOLVING POWER

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7
Q

when two substances have a
different index of refraction, the
light will bend as it passes from
one material to another

A

REFRACTIVE INDEX

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8
Q

Has one lens, similar to a
magnifying glass

A

SIMPLE MICROSCOPE

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9
Q

Leeuwenhoek’s simple
microscopes magnify images
from 100X to 300X

A

SIMPLE MICROSCOPE

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10
Q

Has series of lenses

A

COMPOUND MICROSCOPE

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11
Q

Enables us to examine
very small specimens
and some fine details

A

COMPOUND MICROSCOPE

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12
Q

Microscope uses visible light to
observe specimen

A

LIGHT MICROSCOPY

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13
Q

Specimen appears
against a bright
background

Use: various stained
specimens

A

B R I G H T F I E L D

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14
Q
  • Special condenser with
    opaque disk. Light enters
    the objective lens
  • Use: for microorganisms
    which cannot be stained
A

D A R K F I E L D

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15
Q
  • Uses condenser with annular
    diaphragm which allows direct
    light to pass through the
    condenser
  • Use: detailed examination of
    internal structures; does not
    require staining
A

P H A S E - C O N T R A S T

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16
Q
  • Uses a UV or near-UV
    source of illumination
  • Use: observation of
    natural fluorescence and
    immunofluorescence
A

F L U O R E S E N C E

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17
Q
  • Uses different refractive
    indexes to produce image
  • Use: provides 3D images
    of samples
A

DIFFERENTIAL INTERFERENCE CONTRAST

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18
Q
  • Light source and
    condenser is on top,
    above the stage
  • Use: observation of living
    cells under more natural
    conditions
A

I N V E R T E D

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19
Q

Microscope uses beam of electrons
instead of light

A

ELECTRON MICROSCOPY

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20
Q

Offers much greater resolving power

A

ELECTRON MICROSCOPY

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21
Q

Electrons are reflected from
the specimen; magnifies
1,000 to 10,000X

A

SCANNING ELECTRON (SEM)

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22
Q

Use: study of surface
features of cells and viruses

A

SCANNING ELECTRON (SEM)

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23
Q

Electrons pass through the
specimen; magnifies 10,000
to 100,000X

A

TRANSMISSION ELECTRON (TEM)

24
Q

Use: examination of viruses
or internal ultrastructure in
thin sections of cells

A

TRANSMISSION ELECTRON (TEM)

25
Uses a thin metal probe that scans a specimen
SCANNING TUNNELING (STEM)
26
Use: reveals bumps and depressions of atoms on surface of specimen
SCANNING TUNNELING (STEM)
27
Measures the deflection of a laser beam aimed at the tip of a probe that travels across the surface of the specimen
ATOMIC FORCE MICROSCOPY
28
Measures the deflection of a laser beam aimed at the tip of a probe that travels across the surface of the specimen
ATOMIC FORCE MICROSCOPY
29
Use: visualization of individual molecules and atoms
ATOMIC FORCE MICROSCOPY
30
Used when there is a need to culture microorganisms and for substances that must be protected from contamination
SCREW CAPPED TUBES
31
Used to make broth, agar deep, and agar slant
SCREW CAPPED TUBES
32
Used to detect production of gas by microorganisms. An inverted small tube is initially filled with the solution in which the microorganism is to be grown.
DURHAM FERMENTATION TUBE
33
Simpler than Durham tube. Used also for observing microorganisms perform fermentation.
SMITH FERMENTATION TUBE
34
used to make agar plates for microbiology studies
PETRI DISH
35
Glassware calibrated for precise volume.
VOLUMETRIC FLASK
36
Used for measuring or storing different amounts of liquid
VOLUMETRIC FLASK
37
Has a single graduation that allows it to deliver one specific volume accurately
VOLUMETRIC PIPETTE
38
serological or blow-out pipettes have no 0ml mark as that corresponds to an empty pipette
SEROLOGIC PIPETTE
39
Makes it easy to deliver liquids, especially small volumes, accurately and precisely
PIPETTOR
40
Has a disposable tip
PIPETTOR
41
Used to observe specimens for a long time because they do not dry up very quickly. Thus, motility of an organism can be observed.
HANGING DROP SLIDE
42
AKA smear loop, inoculation wand or microstreaker
INOCULATING LOOP
43
used to retrieve and transfer an inoculum from a culture of microorganisms
INOCULATING LOOP
44
mainly used to pick a single colony (pure) and transfer in an agar slant (stab and streak method)
INOCULATING NEEDLE
45
- Test tube rack - Slant rack - Staining rack
RACKS
46
Uses dry heat to sterilize heat-stable materials
HOT AIR STERILIZER
47
For sterilization of media and other materials affected by moisture
HOT AIR STERILIZER
48
Uses moist heat or steam under pressure
AUTOCLAVE
49
For sterilization of media and other materials affected by moisture
AUTOCLAVE
50
Not sterile and cannot be used to sterilize culture media
DRYING OVEN
51
For drying specimens and lab apparatus or glassware
DRYING OVEN
52
used for growing bacterial cultures or providing suitable conditions for a chemical or biological reaction.
INCUBATOR
53
It is used in the microbiological lab for incubations or enabling chemical reactions to occur at higher temperatures
WATER BATH
54
Storage for culture media
R.REFRIGERATOR
55
Preserves the morphological characteristics of microorganisms while arresting their growth
REFRIGERATOR
56
An enclosed, ventilated lab workspace for safely working with pathogens requiring a defined biosafety level
BIOSAFETY CABINET (BSC)