Biochemical Activities of Bacteria

Question 1

Sum of all chemical reaction that occur within a living cell

Answer 1

Metabolism

Question 2

Metabolic activity of bacteria in the presence of enzymes

Answer 2

Endoenzymes and exoenzymes

Question 3

Energy-producing reactions of bacteria

Answer 3

Oxidative process and fermen

Question 4

Biochemical activities

Answer 4

Carbohydrate fermentation, Organic Nitrogen Metabolism, Electron Transport

Question 5

The substrate formed from the metabolism of carbohydrate is either glucose or lactose

Answer 5

Carbohydrate Fermentation

Question 6

Glucose in Carbohydrate Fermentation

Answer 6

acid, alcohol, gas, organic molecule

Question 7

Culture Media used in Fermentation in Broth Medium

Answer 7

Lactose Broth

Question 8

(Fermentation in Broth Medium) From RED to RED

Answer 8

inability to utilize carbohydrates (negative result)

Question 9

(Fermentation in Broth Medium) from RED to RED (cloudy)

Answer 9

Turbidity indicates growth in the medium, color unchanged indicates other possible sources of energy such as peptone (negative result)

Question 10

(Fermentation in Broth Medium) from RED to YELLOW

Answer 10

Ability to use carbohydrates and produce organic acid (positive result)

Question 11

(Fermentation in Broth Medium) from RED to YELLOW with bubble in Durham tube

Answer 11

Ability to use carbohydrate and produce organic acids and gas (positive result)

Question 12

Culture Media used in Fermentation in Solid Medium?

Answer 12

TSI Agar Deep Slant Tube

Question 13

(Fermentation in Solid Medium) indicated acid production

Answer 13

RED-YELLOW

Question 14

(Fermentation in Solid Medium) indicates hydrogen sulfide gas

Answer 14

BLACK

Question 15

(Fermentation in Solid Medium) YELLOW deep and RED slant

Answer 15

POSITIVE (glucose fermentation)

Question 16

(Fermentation in Solid Medium) YELLOW deep and YELLOW slant

Answer 16

POSITIVE (indicates glucose, lactose, and sucrose fermentation)

Question 17

(Fermentation in Solid Medium) RED deep and RED slant

Answer 17

NEGATIVE (no sugar was fermented)

Question 18

Culture Media used with the Methyl Red Test and Voges-Proskauer Test??

Answer 18

Methyl Red-Voges Proskauer Broth (MRVP)

Question 19

(Methyl Red Test) pH of Glucose acid?

Answer 19

4.4 and below

Question 20

(Methyl Red Test) pH indicator?

Answer 20

Methyl red

Question 21

(Methyl Red Test) yellow at what pH?

Answer 21

6.2

Question 22

(Voges-Proskauer Test) Glucose —>

Answer 22

Acetoin (Acetyl Methyl Carbinol) through Butanediol fermentation

Question 23

Barritt’s A (alpha-napthol) in ethanol

Answer 23

12 gtts

Question 24

Barritt’s B (40% KOH)

Answer 24

6 gtts

Question 25

Used to differentiate between organisms that produce large amounts of acid and organisms that only produce neutral content (acetoin)

Answer 25

MRVP Test

Question 26

Culture Media used in Citrate Utilization Test

Answer 26

Simmons Citrate Agar (SCA) Slants

Question 27

Indicator used in Citrate Utilization Test?

Answer 27

Bromythol blue

Question 28

Alkaline pH in Citrine Utilization Test?

Answer 28

blue

Question 29

Neutral pH in Citrate Utilization Test

Answer 29

green

Question 30

Culture Media Used in Indole Production Test

Answer 30

Sulfide Indole Motility (SIM)

Question 31

Culture Media used in Lysine Decarboxylase Test

Answer 31

Lysine Iron Agar (LIA) Slants

Question 32

(Lysine Decarboxylase Test) lysine ---->

Answer 32

cadaverine and CO2 through decarboxylase

Question 33

(Lysine Decarboxylase Test) Indicator used?

Answer 33

Bromcresol purple

Question 34

(Lysine Decarboxylase Test) Alkaline pH

Answer 34

Purple (positive result)

Question 35

(Lysine Decarboxylase Test) Acidic pH

Answer 35

Yellow

Question 36

(Lysine Decarboxylase Test) H2S production

Answer 36

Black

Question 37

Culture Media used for Phenylalanine Deamination

Answer 37

Phenylalanine Agar Slants

Question 38

(Phenylalanine Deamination) Phenylalanine ------>

Answer 38

phenyl pyruvic acid through phenylalanine deaminase

Question 39

(Phenylalanine Deamination) Reagent contains...

Answer 39

FeCl3

Question 40

(Phenylalanine Deamination) id acid is present it turns the medium

Answer 40

Green

Question 41

(Phenylalanine Deamination) if there is negative acid present it turns the medium

Answer 41

Yellow

Question 42

Culture Media used for Urease Test

Answer 42

Urea Broth

Question 43

(Urease Test) a byproduct of metabolism

Answer 43

Urea

Question 44

(Urease Test) Production of ammonia results in the _____ of the pH of the medium

Answer 44

Increase

Question 45

(Urease Test) Indicator used?

Answer 45

Phenol red

Question 46

(Urease Test) pH<6.8

Answer 46

orange-yellow

Question 47

(Urease Test) pH >8.1

Answer 47

bright pinkish-red

Question 48

(Catalase Test) one of the by-products of oxidation-reduction in the presence of oxygen

Answer 48

Hydrogen peroxide

Question 49

(Catalase Test) H2O2 ----->

Answer 49

water and oxygen through catalase

Question 50

(Functions of Catalase) protects bacteria from...

Answer 50

Toxic hydrogen peroxide (H2O2) accumulation which can occur during aerobic metabolism

Question 51

Anaerobic respiration involves reduction to inorganic compounds other than O2 act as final electron acceptors

Answer 51

Nitrogen Reduction Test

Question 52

(Nitrate Reduction Test) used to determine a bacterium's ability to reduce nitrates

Answer 52

Nitrate broth

Question 53

(Nitrate Reduction Test Reagent) dimethyl-alpha-napthylamine

Answer 53

Nitrate Reagent A

Question 54

(Nitrate Reduction Test Reagent) sulfanilic acid

Answer 54

Nitrate Reagent B