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Microbiology and Parasitology (Lab) > Experiment Results Reviewer > Flashcards

Experiment Results Reviewer Flashcards

(84 cards)

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1
Q

Gram Stain positive result?

A

Purple

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2
Q

Gram Stain negative result?

A

Red

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3
Q

Acid Fast?

A

Pink/Red

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4
Q

Non Acid Fast?

A

Blue/Purple

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5
Q

Negative Staining?

A

White MO against black stain

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6
Q

(Flagella Staining) Wet Mount Staining?

A

Dark purple MO against purple stain

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7
Q

(Flagella Staining) Leifson’s Staining?

A

Blue MO against red stain

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8
Q

(Endospore Staining) Endospore

A

Green

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9
Q

(Endospore Staining) Vegetative Cell

A

Pink

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10
Q

Capsule Staining results?

A

Purple stain, clear capsule, purple MO

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11
Q

(Carbohydrate Fermentation) Culture Media Used?

A

Lactose Broth and TSI and Agar Deep Slant Tube

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12
Q

(Carbohydrate Fermentation, broth medium) Red to red?

A

negative

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13
Q

(Carbohydrate Fermentation, broth medium) Red to cloudy red?

A

some growth but still negative

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14
Q

(Carbohydrate Fermentation, broth medium) Red to yellow?

A

positive with organic acids

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15
Q

(Carbohydrate Fermentation, broth medium) Red to yellow with bubble in Durham tube

A

positive with organic acids and gas

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16
Q

(Carbohydrate Fermentation, solid medium) Yellow deep to red slant?

A

positive with glucose fermentation

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17
Q

(Carbohydrate Fermentation, solid medium) Yellow deep to yellow slant?

A

positive with glucose, lactose, and sucrose fermentation

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18
Q

(Carbohydrate Fermentation, solid medium) Red deep to red slant?

A

negative with no sugar fermentation

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19
Q

(Methyl Red Test and Vogues-Proskauer Test) Culture Media used?

A

Methyl Red-Voges Proskauer Broth

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20
Q

(Methyl Red Test) Positive result?

A

Red

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21
Q

(Methyl Red Test) Negative result?

A

Yellow

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22
Q

(Voges-Proskauer Test) Positive result?

A

Red

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23
Q

(Voges-Proskauer Test) Negative result?

A

Yellow

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24
Q

(Voges-Proskauer Test) Negative result?

A

Yellow

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25
(Citrate Utilization Test) Culture Media Used?
Simmons Citrate Agar (SCA) Slants
26
(Citrate Utilization Test) Positive result?
Blue indicates an alkaline pH
27
(Citrate Utilization Test) Negative result?
Green indicates a neutral pH
28
(Indole Production Test) Culture Media Used?
Sulfide Indole Motility (SIM)
29
(Indole Production Test) purple color of kovac's reagent, no black, but turbid
indole positive, sulfide negative, motile
30
(Indole Production Test) purple color of kovac's medium, black
indole positive, sulfide and motile positive
31
(Indole Production Test) yellow and has sharp line of growth
all negative reactions
32
(Lysine Decarboxylase Test) Culture Media Used?
Lysine Iron Agar (LIA) Slants
33
(Lysine Decarboxylase Test) positive result?
purple and has alkaline pH
34
(Lysine Decarboxylase Test) negative result?
yellow and has acidic pH
35
(Lysine Decarboxylase Test) presence of hydrogen sulfide?
black
36
(Phenylalanine Deamination) Culture Media Used?
Phenylalanine Agar Slants
37
(Phenylalanine Deamination) positive result?
Green which means an acid is present
38
(Phenylalanine Deamination) negative result?
Yellow and acid is not present
39
(Urease Test) Culture Media Used?
Urea Broth
40
(Urease Test) positive result?
bright pinkish-red is pH>8.1
41
(Urease Test) negative result?
orange-yellow is pH<6.8
42
(Catalase Test) positive result?
formation of bubbles
43
Purple
Gram Stain positive result?
44
Red
Gram Stain negative result?
45
Pink/Red
Acid Fast?
46
Blue/Purple
Non Acid Fast?
47
White MO against black stain
Negative Staining?
48
Dark purple MO against purple stain
(Flagella Staining) Wet Mount Staining?
49
Blue MO against red stain
(Flagella Staining) Leifson's Staining?
50
Green
(Endospore Staining) Endospore
51
Pink
(Endospore Staining) Vegetative Cell
52
Purple stain, clear capsule, purple MO
Capsule Staining results?
53
Lactose Broth and TSI and Agar Deep Slant Tube
(Carbohydrate Fermentation) Culture Media Used?
54
negative
(Carbohydrate Fermentation, broth medium) Red to red?
55
some growth but still negative
(Carbohydrate Fermentation, broth medium) Red to cloudy red?
56
positive with organic acids
(Carbohydrate Fermentation, broth medium) Red to yellow?
57
positive with organic acids and gas
(Carbohydrate Fermentation, broth medium) Red to yellow with bubble in Durham tube
58
positive with glucose fermentation
(Carbohydrate Fermentation, solid medium) Yellow deep to red slant?
59
positive with glucose, lactose, and sucrose fermentation
(Carbohydrate Fermentation, solid medium) Yellow deep to yellow slant?
60
negative with no sugar fermentation
(Carbohydrate Fermentation, solid medium) Red deep to red slant?
61
Methyl Red-Voges Proskauer Broth
(Methyl Red Test and Vogues-Proskauer Test) Culture Media used?
62
Red
(Methyl Red Test) Positive result?
63
Yellow
(Methyl Red Test) Negative result?
64
Red
(Voges-Proskauer Test) Positive result?
65
Yellow
(Voges-Proskauer Test) Negative result?
66
Yellow
(Voges-Proskauer Test) Negative result?
67
Simmons Citrate Agar (SCA) Slants
(Citrate Utilization Test) Culture Media Used?
68
Blue indicates an alkaline pH
(Citrate Utilization Test) Positive result?
69
Green indicates a neutral pH
(Citrate Utilization Test) Negative result?
70
Sulfide Indole Motility (SIM)
(Indole Production Test) Culture Media Used?
71
indole positive, sulfide negative, motile
(Indole Production Test) purple color of kovac's reagent, no black, but turbid
72
indole positive, sulfide and motile positive
(Indole Production Test) purple color of kovac's medium, black
73
all negative reactions
(Indole Production Test) yellow and has sharp line of growth
74
Lysine Iron Agar (LIA) Slants
(Lysine Decarboxylase Test) Culture Media Used?
75
purple and has alkaline pH
(Lysine Decarboxylase Test) positive result?
76
yellow and has acidic pH
(Lysine Decarboxylase Test) negative result?
77
black
(Lysine Decarboxylase Test) presence of hydrogen sulfide?
78
Phenylalanine Agar Slants
(Phenylalanine Deamination) Culture Media Used?
79
Green which means an acid is present
(Phenylalanine Deamination) positive result?
80
Yellow and acid is not present
(Phenylalanine Deamination) negative result?
81
Urea Broth
(Urease Test) Culture Media Used?
82
bright pinkish-red is pH>8.1
(Urease Test) positive result?
83
orange-yellow is pH<6.8
(Urease Test) negative result?
84
formation of bubbles
(Catalase Test) positive result?

Microbiology and Parasitology (Lab) (6 decks)

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