Biochemical Tests Flashcards Preview

Davis BIO 225 (Lab) > Biochemical Tests > Flashcards

Flashcards in Biochemical Tests Deck (19)
Loading flashcards...
1
Q

The superoxide ion is first converted to hydrogen peroxide and normal oxygen by the action of an enzyme called

A

Superoxide dismutase.

2
Q

Hydrogen peroxide must be degraded by an enzyme called _____ into water and oxygen

A

Catalase

3
Q

A catalase positive test will have what reaction?

A

It will bubble

4
Q

Used to identify bacteria containing the respiratory enzyme cytochrome c oxidase

A

Oxidase Test

5
Q

An oxidase positive test will turn what color

A

Blue

6
Q

What is the time limit for interpretation of an Oxidase test

A

20 Seconds

7
Q

The SIM medium is used for determination of which three bacterial activities

A

Sulfur Reduction, Indole Production and Motility

8
Q

During a SIM test how will you know that sulfur reduction occurred?

A

The media will turn black

9
Q

During a SIM test if a thick stab line is present than your bacteria most likely have a______

A

Flagella

10
Q

Tryptophan is broken down into indole and pyruvate by which enzyme?

A

The tryptophanase enzyme

11
Q

What is the name of the reagent used to check for indole production?

A

Kovacs Reagent

12
Q

Indole is a by product of the break down of what?

A

Tryptophan

13
Q

Some organisms float on top of the medium and produce a type of surface membrane called a

A

Pellicle

14
Q

Some organisms sink to the bottom of a nutrient broth. This is called a

A

Sediment

15
Q

Growth at the top around the edges of a nutrient broth is known as a

A

Ring

16
Q

In a nutrient broth where will Aerobic bacteria grow?

A

Towards the top surface

17
Q

In a nutrient broth where will Anaerobic bacteria grow?

A

At the bottom of the test tube

18
Q

In a nutrient broth where will facultative anaerobic bacteria grow?

A

At both the top and bottom of the test tube

19
Q

Cloudiness in a nutrient broth is known as

A

Turbidity