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Flashcards in Biochemical Tests Deck (19)
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1

The superoxide ion is first converted to hydrogen peroxide and normal oxygen by the action of an enzyme called

Superoxide dismutase.

2

Hydrogen peroxide must be degraded by an enzyme called _____ into water and oxygen

Catalase

3

A catalase positive test will have what reaction?

It will bubble

4

Used to identify bacteria containing the respiratory enzyme cytochrome c oxidase

Oxidase Test

5

An oxidase positive test will turn what color

Blue

6

What is the time limit for interpretation of an Oxidase test

20 Seconds

7

The SIM medium is used for determination of which three bacterial activities

Sulfur Reduction, Indole Production and Motility

8

During a SIM test how will you know that sulfur reduction occurred?

The media will turn black

9

During a SIM test if a thick stab line is present than your bacteria most likely have a______

Flagella

10

Tryptophan is broken down into indole and pyruvate by which enzyme?

The tryptophanase enzyme

11

What is the name of the reagent used to check for indole production?

Kovacs Reagent

12

Indole is a by product of the break down of what?

Tryptophan

13

Some organisms float on top of the medium and produce a type of surface membrane called a

Pellicle

14

Some organisms sink to the bottom of a nutrient broth. This is called a

Sediment

15

Growth at the top around the edges of a nutrient broth is known as a

Ring

16

In a nutrient broth where will Aerobic bacteria grow?

Towards the top surface

17

In a nutrient broth where will Anaerobic bacteria grow?

At the bottom of the test tube

18

In a nutrient broth where will facultative anaerobic bacteria grow?

At both the top and bottom of the test tube

19

Cloudiness in a nutrient broth is known as

Turbidity