Simple Staining and Negative Staining Flashcards Preview

Davis BIO 225 (Lab) > Simple Staining and Negative Staining > Flashcards

Flashcards in Simple Staining and Negative Staining Deck (18)
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1

The portion of the chromogen that gives it color is called the

Chromophore

2

The charged portion of a chromogen is known as the

Auxochrome

3

Stains consist of a ______ , and a colored molecule called the _____

Solvent;Chromogen

4

The two common dyes are

Basic Dyes and Acidic Dyes

5

Basic dyes have a _____ charge and stain the_____

Positive; Cells

6

Acidic dyes have a ______ charge and stain the____

Negative; Background

7

Why are acidic dyes repelled by bacterial cell membranes?

Because bacterial cells also have a negative charge

8

Simple stains use what type of dye?

Basic Dye

9

Simple stains show these three physical characteristics

Size, Shape and Arrangement

10

Circular shaped bacterial cells are

Coccus

11

Rod shaped bacterial cells are

Bacillus

12

Pairs of cells form this arrangement

Diplo

13

Irregular clusters form this arrangement

Staphylo

14

Chains of cells form this arrangement

Strepto

15

What three things does heat fixation accomplish

1. It kills the organisms 2. It cause the organism to adhere to the slide 3. It alters the organism so that they can more readily accept stains

16

When are negative stains useful

To determine morphology and cellular arrangement in bacteria that are to delicate to withstand heat fixing.

17

Negative staining requires which type of dye

Acidic Dye

18

What gets stained during a negative staining procedure

The background