Simple Staining and Negative Staining Flashcards Preview

Davis BIO 225 (Lab) > Simple Staining and Negative Staining > Flashcards

Flashcards in Simple Staining and Negative Staining Deck (18)
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1
Q

The portion of the chromogen that gives it color is called the

A

Chromophore

2
Q

The charged portion of a chromogen is known as the

A

Auxochrome

3
Q

Stains consist of a ______ , and a colored molecule called the _____

A

Solvent;Chromogen

4
Q

The two common dyes are

A

Basic Dyes and Acidic Dyes

5
Q

Basic dyes have a _____ charge and stain the_____

A

Positive; Cells

6
Q

Acidic dyes have a ______ charge and stain the____

A

Negative; Background

7
Q

Why are acidic dyes repelled by bacterial cell membranes?

A

Because bacterial cells also have a negative charge

8
Q

Simple stains use what type of dye?

A

Basic Dye

9
Q

Simple stains show these three physical characteristics

A

Size, Shape and Arrangement

10
Q

Circular shaped bacterial cells are

A

Coccus

11
Q

Rod shaped bacterial cells are

A

Bacillus

12
Q

Pairs of cells form this arrangement

A

Diplo

13
Q

Irregular clusters form this arrangement

A

Staphylo

14
Q

Chains of cells form this arrangement

A

Strepto

15
Q

What three things does heat fixation accomplish

A
  1. It kills the organisms 2. It cause the organism to adhere to the slide 3. It alters the organism so that they can more readily accept stains
16
Q

When are negative stains useful

A

To determine morphology and cellular arrangement in bacteria that are to delicate to withstand heat fixing.

17
Q

Negative staining requires which type of dye

A

Acidic Dye

18
Q

What gets stained during a negative staining procedure

A

The background