Biochemistry - Cardiology Block (III) Flashcards
(167 cards)
1
Q
Why is hemoglobin a necessary component of efficient oxygen transport within the body?
A
O2 is relatively insoluble
(as are all nonpolar gases)
2
Q
Which is more soluble in blood (and any aqueous solution), polar or nonpolar gases?
A
Polar gases
3
Q
At what pH is O2 / hemoglobin binding increased, high or low?
A
High pH
4
Q
At what pH is O2 / hemoglobin binding decreased, high or low?
A
Low pH
5
Q
Which of the following affect myoglobin affinity for O2?
pH
2,3-DPG
CO2
A
None of them
(myoglobin not regulated by pH or modifying molecules as hemoglobin is)
6
Q
Hemoglobin is made up of how many subunits?
Myoglobin is made up of how many subunits?
A
4;
1
7
Q
What are the two contributing portions of a heme molecule?
A
The protoporphyrin IX ring
+
Fe2+ (ferrous iron)
8
Q
How many bonds can a single ferrous iron atom make?
To what structure(s) is one ferrous iron atom bound in a single heme molecule?
A
6;
4 bonds to the protoporphyrin IX ring,
1 bond to a posterior histidine (from helix F),
1 potential bond to oxygen
9
Q
Binding of O2 to the ferrous iron in a heme molecule leads to _______ movement of what amino acid also bound to the ferrous iron?
A
Lateral;
histidine
10
Q
Ferrous iron is notated as ___?
Is this an oxidized or reduced form of Fe?
A
Fe2+;
reduced (as opposed to oxidized, ferric iron –> Fe3+)
11
Q
True/False.
Ligand binding to molecules such as hemoglobin is all of the following: specific, reversible, and transient.
A
True.
12
Q
What is the Kd in regards to ligands for a molecule like myoglobin?
A
The concentration [L] of the ligand (mM) at which 50% of the available binding sites are occupied (on average)
13
Q
A ligand with high affinity for its substrate protein (e.g. hemoglobin) will have what type of Kd, high or low?
A
Low
14
Q
A Kd in ligand binding is equivalent to what type of constant in Michaelis-Menten kinetics?
A
Km
- (low Kd = high affinity;*
- high Kd = low affinity)*
15
Q
What does sigma represent in the attached graph?
(Note: the graph shows myoglobin O2 binding in relation to partial pressure of O2)
A
θ = binding sites filled
(in this case, by O2)
16
Q
Which is indicative of high ligand affinity in a graph comparing binding sites to partial pressure of the gas in question, a right shift or a left shift?
A
Left shift
17
Q
A right shift in a graph showing hemoglobin-O2 binding would indicate what type of affinity, high or low?
A
Low
18
Q
The hemoglobin-O2 binding curve is ___________, indicating cooperativity.
The myoglobin-O2 binding curve is ___________, indicating no cooperativity.
A
Sigmoidal;
hyperbolic
19
Q
The myoglobin-O2 binding curve is hyperbolic, indicating no __________.
The hemoglobin-O2 binding curve is sigmoidal, indicating __________.
A
Cooperativity;
cooperativity
20
Q
In the T state, the Fe2+ in heme is where?
A
Pulled out of the plane of the protoporphyrin IX ring
(low affinity for O2)
21
Q
In the R state, the Fe2+ in heme is where?
A
In-plane with the protoporphyrin IX ring
(high affinity for O2)
22
Q
The histidine that binds the heme group Fe2+ is attached to which hemoglobin subunit, α or β?
A
α
(from helix F)
23
Q
In which hemoglobin state (tense or relaxed) is the Fe2+ pulled out of plane with the protoporphyrin ring (by histidine)?
A
Tense (T)
24
Q
In which hemoglobin state (tense or relaxed) is the Fe2+ pulled into plane with the protoporphyrin ring (by histidine)?
A
Relaxed (R)
25
How many O2 molecules can bind one hemoglobin molecule?
4
26
What type of bonds allow for hemoglobin conformational change from T to R and back?
How many of these bonds?
Ionic bonds;
8 (2 intrachain; 6 interchain)
27
For each single O2 molecule that binds hemoglobin, how many ionic bonds are broken (of the 8 involved in conformational change)?
2
28
Describe which concentration changes in the following would likely lead to a right shift (decreased affinity) in hemoglobin-O2 binding:
H+
2,3-BPG
CO2
CO
H+ - **Increased**
2,3-BPG - **Increased**
CO2 - **Increased**
CO - **Decreased**
29
How does increased [H+] lead to increased O2 unloading from hemoglobin?
Decreased pH leads to stronger ionic interactions between hemoglobin subunits (favoring the T state)
30
Describe which concentration changes in the following would likely lead to a left shift (increased affinity) in hemoglobin-O2 binding:
H+
2,3-BPG
CO2
CO
H+ - **Decreased**
2,3-BPG - **Decreased**
CO2 - **Decreased**
CO - **Increased**
31
In what direction does a high CO concentration shift the hemoglobin-O2 binding curve?
To the left
| (high CO increases Hb-O2 binding)
32
Does 2,3-BPG increase or decrease O2 binding to hemoglobin?
Decrease
| (causes O2 unloading)
33
Describe the three methods by which CO2 is transported in the blood and what percentage of CO2 follows each method.
Dissolved in the blood (10%)
As carbaminohemoglobin (~20%)
As bicarbonate (~70%)
34
How much of CO2 is simply dissolved in the blood?
10%
35
How much of CO2 is transported as carbaminohemoglobin?
~20%
36
How much of CO2 is transported as bicarbonate?
~70%
37
Where does CO2 bind hemoglobin to form carbaminohemoglobin?
The amino-terminal end of each subunit
38
What two effects does CO have on O2-hemoglobin interactions?
1. It replaces O2
(binds with 250x the affinity of O2)
2. It causes a left shift and decreased O2 unloading
(stabilizes the R state)
39
How does CO poisoning manifest?
How does severe CO poisoning manifest?
How can CO poisoning be treated?
Flu-like syndromes (headache, nausea, vertigo);
seizures, coma and death;
pure oxygen delivery (can be hyperbaric)
40
Ingestion of nitrates (e.g. bismuth nitrate or from well water) or oxidixing medications can have what effect on hemoglobin?
What is this condition called in infants?
How is it treated (in general)?
Oxidation of Fe2+ to Fe3+
(methemoglobinemia);
blue-baby syndrome;
methylene blue
41
Prolonged periods of time spent at high altitudes will have what two effects on the blood?
Increased 2,3-BPG;
increased Hb and RBCs
42
How are γ Hb subunits different from β Hb subunits?
*(I.e. how are infant hemoglobin* (α2γ2) *different from adult hemoglobin (*α2β2)*?)*
Less affinity for 2,3-BPG
*(specifically, 2 serine residues are replaced with 2 histidine granules)*
43
Why does fetal Hb (α2γ2) have a higher affinity for O2 than adult Hb (α2β2)?
γ subunits has less affinity for 2,3-BPG than β subunits
44
What is the mechanism of the Bohr effect?
As **acidity increases**, protons attach to hemoglobin histidines
---\> cause a conformational change in Hgb and **O2 unloading**
45
What enzyme combines CO2 and H2O to form carbonic acid and a proton?
Which is the predominant tissue in which this reaction take place?
Carbonic anhydrase;
erythrocytes
46
How much of CO2 is transported in the blood as bicarbonate?
How much of CO2 is transported in the blood as dissolved CO2?
How much of CO2 is transported in the blood bound to Hgb as carbaminohemoglobin?
~70%
~10%
~20%
47
Which has a stronger affinity for Hgb, O2 or CO?
How much stronger is its affinity?
CO
250x
48
**True/False.**
A single bound CO destabilizes the R state of Hgb.
**False**.
It stabilizes the Hgb
49
Sickle cell anemia results due to what genetic defect?
Glutamate6 change to valine6 in _both β-hemoglobin chains_
50
**True/False.**
A single mutation in the gene coding for the sixth glutamic acid in β-hemoglobin is all it takes to result in sickle cell anemia.
**False**.
The disorder is autosomal recessive. A mutation in both genes (SS, homozygous) is necessary.
51
Sickle cell anemia results from a mutation in the __ glutamic acid in β-Hgb to \_\_\_\_\_\_\_\_.
Hemoglobin C disease results from a mutation in the __ glutamic acid in β-Hgb to \_\_\_\_\_\_\_\_.
6th, valine;
6th, lysine
52
**True/False.**
Sickle cell vasocclusive crises can affect any organ system.
True.
53
What inheritance pattern does sickle cell anemia follow?
Autosomal recessive
54
What is the first-line treatment for the disorder shown here?
Via what mechanism does it function?
Hydroxyurea;
increasing HgbF concetrations
55
HgbS is most likely to form fibers and sickle RBCs when it is in what state?
What are the implications?
The deoxygenated state;
hypoxic conditions (e.g. exercise, high altitudes) can exacerbate the condition
56
Through what mechanism does hydroxyurea improve the signs/symptoms of sickle cell anemia?
HgbF levels increase;
HgbF gets incorporated into HgbS chains and halts their continued polymerization
57
Is sickle cell anemia a hemolytic anemia?
Yes.
The sickle cells are rigid and inflexible and become jammed and/or lysed in capillary beds
58
Besides hydroxyurea, what other treatments exist for sickle cell anemia in certain situations?
Blood transfusions,
prophylactic antibiotics,
pain management,
bone marrow transplants
59
The glutamic acid that is changed to valine in sickle cell anemia is at what amino acid position and in which hemoglobin chain?
The glutamic acid that is changed to lysine in hemoglobin C disease is at what amino acid position and in which hemoglobin chain?
6, β-Hgb;
6, β-Hgb
60
What treatments are typically needed for hemoglobin C disease?
**None**;
it is basically a much less severe form of sickle cell anemia
(glutamic acid6 --\> lysine6 instead of valine)
61
What form of HbS (Sickle cell hemoglobin) is protective against malaria?
The heterozygous form
62
During weeks 3 - 8 of embryonic development, what organ is producing erythrocytes?
What kind of hemoglobin is produced? What two subunits are used?
Yolk sac;
hemoglobin E (HbE), ε2ζ2 (epsilon, zeta)
63
At week 9 of embryonic development, which organ takes over erythrocyte production?
What kind of hemoglobin is produced? What two subunits are used?
The liver;
hemoglobin F (HbF), α2γ2
64
What are the three main types of hemoglobin found in adults?
In what percentages?
Adult Hb (HbA) **α2β2** \>95% of adult Hb
Adult Hb (HbA2) **α2δ2** \<3% of adult Hb
Fetal Hb (HbF) **α2γ2** \<3% of adult Hb
65
What are the two forms of adult hemoglobin?
HbA **α2β2** (\>95%)
HbA2 **α2δ2 **
66
What type of hemoglobin predominates in the embryonic period?
What type of hemoglobin predominates in the fetal period?
What type of hemoglobin predominates in the adult period?
What type of hemoglobin is present in adults but never predominates at any stage?
HbE **ε2ζ2**
HbF **α2γ2**
HbA **α2β2**
HbA2 **α2δ2**
67
About 5% of adult hemoglobin does not come from HbA (α2β2). This 5% comes from a mix of what two other types of hemoglobin?
HbA2 **α2δ2**
HbF **α2γ2**
68
Although most cases of methemoglobinemia result from exposure to nitrates or certain drugs, what two genetic changes can also cause it?
Certain mutations in the α- or β-globin chains (autosomal dominant);
mutated NADH-cytochrome B5 reductase (diaphorase I) (reduces met-Hb to Hb in normal cells)
69
What enzyme is responsible for reducing methemoglobin back to hemoglobin in normal RBCs?
NADH-cytochrome B5 reductase (diaphorase I)
70
Hemoglobin normally has iron in its ___________ (oxidized/reduced) state, Fe?.
Methemoglobin has iron in its ___________ (oxidized/reduced) state, Fe?.
Reduced, Fe2+ (ferrous);
oxidized, Fe3+ (ferric)
71
**True/False.**
Individuals with sickle cell anemia are at an increased risk for infection.
True.
72
What is a very prominent sign of methemoglobinemia?
What is this called when found in infants?
Blue-brown skin;
blue baby syndrome
73
What are the two main causes of 'blue baby syndrome?'
Methemoglobinemia;
congenital cyanotic heart diseases
74
**True/False.**
Some individuals have a congenitally high level of HbF (α2γ2) as high as 30%.
**True**.
Hereditary persistence of fetal hemoglobin (HPFH)
75
What are the two types of α-globin chain?
What are the two types of β-globin chain?
**α** (alpha), **ζ** (zeta)
**β** (beta), **γ** (gamma), **δ** (delta), **ε** (epsilon)
76
Describe the transition in hemoglobin type from yolk sac production to post-natal hemoglobin.
77
How is methemoglobinemia treated?
IV methylene blue
78
What gene can be edited to increase HbF (α2γ2) and improve the symptoms of sickle cell anemia?
HS-1 (γ-silencing region)
79
# Define thalassemia.
What inheritance pattern do thalassemias follow?
A mutation that diminishes or eliminates the production of one of the two chains of hemoglobin;
autosomal recessive
80
What population(s) is most affected by β-thalassemia?
What population(s) is most affected by α-thalassemia?
Mediterranean;
Southeast Asian, Middle Eastern, African
81
**True/False.**
Thalassemias can be caused by mutations in the coding or regulatory genes for α- or β-globin.
True.
82
How are the hemoglobin genes controlled?
On what chromosome are the genes coding for α-globin located?
On what chromosome are the genes coding for β-globin located?
by **locus control regions**;
16;
11
83
In what order are the various β-globin genes organized on chromosome 11?
In the order in which they become useful
(starting with the locus control region; epsilon --\> gamma --\> delta --\> beta)
84
A newborn presents in the first year of life with severe microcytic hypochromic anemia.
What autosomal recessive disorder might this child have and why are they at an increased risk of heart failure as early as the second or third decade of life?
β°-thalassemia major (Cooley's anemia)
buildup of iron in the heart and other organs
85
A symptomatic patient has both β-hemoglobin genes inactivated. What disorder is this?
An asymptomatic patient has a single β-hemoglobin genes inactivated. What disorder is this?
A symptomatic patient has a single β-hemoglobin genes inactivated. What disorder is this?
β°-thalassemia major (Cooley's anemia);
β-thalassemia minor;
β-thalassemia intermedia
86
What is β+-thalassemia?
Decreased β-hemoglobin production due to a mutation in the locus control region
87
In what order are the various α-globin genes organized on chromosome 16?
In the order in which they become useful
(starting with the locus control region; zeta --\> alpha)
88
What type of α-thalassemia is fatal in-utero and is characterized by virtually no α-globin production? *(All 4 genes deleted)*
What type of heterozygous, severe α-thalassemia is characterized by only 25% of normal α-globin production? *(3 genes deleted)*
What type of mildy symptomatic α-thalassemia presents similarly to an iron deficiency? *(2 genes deleted)*
What type of α-thalassemia is asymptomatic? *(1 gene deleted)*
α-thalassemia major *(hemoglobin Bart) (hydrops fetalis) (Hgb* γ4)
Hemoglobin H disease *(Hgb β4 + Hgb* γ4*)*
α-thalassemia trait (cis or trans)
α-thalassemia silent
89
What type of α-thalassemia is fatal in-utero and is characterized by virtually no α-globin production? *(All 4 genes deleted)*
How many of the 4 α-globin genes (2 alleles each in 2 diploid cells) are deleted in this case?
α-thalassemia major *(hemoglobin Bart) (hydrops fetalis) (Hgb* γ4);
4
90
What type of heterozygous, severe α-thalassemia is characterized by only 25% of normal α-globin production?
How many of the 4 α-globin genes (2 alleles each in 2 diploid cells) are deleted in this case?
Hemoglobin H disease *(Hgb β4 + Hgb* γ4*)*
3
91
What type of mildy symptomatic α-thalassemia presents similarly to an iron deficiency?
How many of the 4 α-globin genes (2 alleles each in 2 diploid cells) are deleted in this case?
α-thalassemia trait (cis or trans)
92
What type of α-thalassemia is asymptomatic?
How many of the 4 α-globin genes (2 alleles each in 2 diploid cells) are deleted in this case?
α-thalassemia silent;
1
93
What are the three most abundant classes of plasma proteins?
Albumin (60%)
Immunoglobulins (18%)
Fibrinogens (4%)
94
What are albumin's two main functions?
To maintain oncotic pressure;
to transport substances in the blood
95
Define antigen.
Foreign molecule that is selectively bound by antibodies
96
Define immunogen.
Molecules that induce antibody production (e.g. carbohydrates, proteins, nucleic acids)
97
# Define antigenic determinant.
What is its alternate name?
A small region of a larger molecule that elicits production of a specific antibody;
**epitope**
98
# Define epitope.
What is its alternate name?
A small region of a larger molecule that elicits production of a specific antibody;
**antigenic determinant**
99
Epitopes (antigenic determinants) are usually only large enough for an immunoglobulin region of what size to bind them?
6 - 7 amino acids
| (the hypervariable regions)
100
Name the different terms that line up with each of the following definitions.
* 1. Molecules that induce antibody production (e.g. carbohydrates, proteins, nucleic acids)*
* 2. A small region of a larger molecule that elicits production of a specific antibody*
* 3. Foreign molecule that is selectively bound by antibodies*
1. Immunogen
2. Antigenic determinant; epitope
3. Antigen
101
Describe the basic structure of an immunoglobulin.
102
Where are the main bonds combining heavy and light chains?
What type of bond are they?
Heavy-heavy (x2)
Heavy-light (x1 each)
103
What are the two types of antibody light chain?
Lambda and kappa
| (λ and κ)
104
The Fc end of the immunoglobulin ends in which terminus?
The antigen-binding end of the immunoglobulin ends in which terminus?
Fc --\> carboxy(C)-terminus
Antigen-binding --\> amino(N)-terminus
105
How many domains make up an antibody heavy chain?
Describe them.
4;
1 variable (VH), 3 constant (CH1, CH2, CH3)
106
How many domains make up an antibody light chain?
Describe them.
2;
1 variable (VL), 1 constant (CL)
107
**True/False.**
A single immunoglobulin molecule can have a kappa light chain and a lambda light chain.
**False**.
While it may have either type, it will have either two kappa light chains or two lamda light chains (i.e. there is no mixing or matching of light chain types)
108
Describe which classes of antibody can take on different forms (e.g. monomer, dimer, tetramer, pentamer, etc.).
109
What is the supersecondary structure of an immunoglobulin?
A beta sandwhich (immunoglobulin fold)
* (constant region: 4-3 arrangement*
* variable region: 5-4 arrangement)*
110
How many complementary determining regions (CDRs) exist per single variable domain (of which, there are four per monomeric antibody)?
3
## Footnote
* (yielding 12 per immunoglobulin monomer and*
* 60 per IgM pentamer)*
111
Describe the structure of an antigen-binding cleft (pocket).
3 heavy chain loops + 3 light chain loops
= 1 barrel-shaped pocket
112
The constant domain will be identical within each ____ of antibody.
Class (IgG, IgA, IgM, IgD, IgE)
113
What defines the class of an antibody?
What are the classes?
The constant domain;
IgG, IgA, IgM, IgD, IgE
114
Name the classes of antibody from most to least prevelant in the serum.
Put their relative domains in parentheses next to them.
**IgG** (γ), 12 mg/ml
**IgA** (α), 3 mg/ml
**IgM** (μ), 1 mg/ml
**IgD** (δ), 0.1 mg/ml
**IgE** (ε), 0.001 mg/ml
115
IgG, IgA, IgM, IgD, and IgE class immunoglobulins are defined by which constant domains, respectively?
**γ** (IgG)
**α** (IgA)
**μ** (IgM)
**δ** (IgD)
**ε** (IgE)
116
Which immunoglobulin class is the first seen in acute immune responses?
IgM
117
Which immunoglobulin is found in mucosal secretions?
IgA
118
Which immunoglobulin class is present on lymphocyte cell surfaces but its function is not yet clear?
IgD
119
Which is the most abundant immunoglobulin class in serum?
IgG
120
Which immunoglobulin class is found most abundantly on mast cell surfaces?
IgE
121
What are complementary determining regions (CDRs)?
How many does each immunoglobulin have?
The part of the antibody that binds an antigen;
**12** (12 possible specificities per antibody)
122
What are some examples of non-immunoglobulin proteins that have similar structural motifs to immunoglobulins?
Fibronectin, cell adhesion molecules, T cell receptors, MHCs
123
What is another name for the complementary determining regions (CDRs) on antibodies?
How many does each immunoglobulin have?
Hypervariable regions;
12
124
What test uses **either** antibodies to look for complementary antigen binding **or** antigens to look for complementary antibody binding?
**ELISA** (enzyme-linked immunosorbent assay)
125
How does a Western blot (immunoblot) work?
Proteins are run on an SDS gel
--\> the gel is incubated in an antibody-containing substance to show presence of a specific protein
126
Attached is an image showing various crossovers between human and mouse (or other animal) immunoglobulins for therapeutic monoclonal antibodies to be produced.
Describe the convention for naming antibodies of these four types.
Murine Antibodies: -momab
Chimeric Antibodies: -ximab
Humanized Antibodies: -zumab
Fully Human Antibodies: -mumab
127
What antibody-mediated test shows the relative amount of a substance in solution?
What antibody-mediated test shows the relative size of a specific protein?
What antibody-mediated test can be used to isolate a single protein-bound DNA sequence for amplification?
ELISA;
immunoblot (Western blot);
Chip
128
A drug ends in -momab. This drug is a ____________ \_\_\_\_\_\_\_\_\_\_\_\_ that is completely \_\_\_\_\_\_\_\_\_\_\_\_.
monoclonal antibody (mab);
murine (mo) (meaning mouse)
129
A drug ends in -ximab. This drug is a ____________ \_\_\_\_\_\_\_\_\_\_\_\_ that is \_\_\_\_\_\_\_\_\_\_\_\_.
monoclonal antibody (mab);
chimeric (xi) (murine/mouse + human)
130
Which of these monoclonal antibodies is the body most likely to mount a resistance against?
**Murine** \> chimerized \>\> humanized \>\>\>\> human
131
A drug ends in -zumab. This drug is a ____________ \_\_\_\_\_\_\_\_\_\_\_\_ that is \_\_\_\_\_\_\_\_\_\_\_\_.
monoclonal antibody (mab);
humanized (zu) (partially murine/mouse)
132
Why is it best to use antibodies with the least amount of murine (mouse) (or other organism) protein involved?
There is less likelihood that the body will mount an immune response against the antibody
133
A drug ends in -mumab. This drug is a ____________ \_\_\_\_\_\_\_\_\_\_\_\_ that is completely \_\_\_\_\_\_\_\_\_\_\_\_.
monoclonal antibody (mab);
human (mu)
134
What are polyclonal antibodies?
What are monoclonal antibodies?
Antibodies produced by various B cells to respond to multiple epitopes on a single antigen (normal bodily function);
antibodies produced by clonal B cells to respond to a single epitope on a single antigen (usually done in culture)
135
Which represents normal bodily antibody production, polyclonal or monoclonal antibodies?
Polyclonal
## Footnote
*(multiple types of plasma cell producing antigens to various epitopes on a target antigen)*
136
Why is monoclonal antibody production safer for clinical/therapeutic use?
As there is only a single epitope target, there is less likelihood of interaction with other bodily tissues
137
What is an example of a disease caused by monoclonal antibody production?
What is this antibody called?
What is found in the urine?
Multiple myeloma;
M protein;
Bence Jones proteins
138
What are Bence Jones proteins?
M protein light chains secreted in the urine
(multiple myeloma)
139
What are the four main signs/symptoms of multiple myeloma?
**CRAB**
**C**a2+ elevation
**R**enal failure
**A**nemia
**B**one lesions
140
Why do the plasma cells in multiple myeloma result in monoclonal antibody production when most antibody production in the body is polyclonal (multiple plasma cell types creating multple antibodies)?
The multiple myeloma cells are immortal and outlast / outcrowd the other plasma cells
141
What are the two largest classes of therapeutics?
1. Vaccines
2. Monoclonal antibodies
142
In what cultures or organisms are most therapeutic monoclonal antibodies produced?
Bacterial or insect cultures
143
What does it mean if a therapeutic agent's name ends in '-mab?'
(E.g. abciximab)
Monoclonal antibody
144
What are the two general categories of reasons a person might have erythrocytosis?
**EPO elevation** *(external cause)*
(e.g. hypoxia [high altitudes, lung disorders]; EPO-secreting tumor; supplemental EPO or androgens);
**consitutional overactivation of JAK/STAT** *(internal cause)*
(e.g. myeloproliferative disorders such as polycythemia vera)
145
What is the JAK protein?
What does it activate?
How does its pathway work?
A tyrosine kinase;
STAT proteins;
EPO --\> myeloid stem cell receptor --\> JAK/STAT activation --\> erythrocyte differentiation
146
What is the purpose of PCR?
What are the three steps?
To amplify DNA fragments;
heating/denaturation, annealing of primers, /replication
147
What is a myeloproliferative disorder involving JAK2 activation and mainly erythrocytosis?
What is a myeloproliferative disorder involving JAK2 activation and mainly thrombocytosis?
What is a myeloproliferative disorder involving JAK2 activation and mainly granulocytosis?
Polycythemia vera;
thrombocytosis;
CML
148
**True/False.**
Bone marrow fibrosis is often associated with JAK2 inactivations and myeloproliferative disorders.
**False**.
Bone marrow fibrosis is often associated with **JAK2 activations** and myeloproliferative disorders.
149
What lab should you order to assist in determining the cause of erythrocytosis?
EPO
150
What is the mnemonic for EPO-secreting tumors?
"Ah, **PHUCK**. I have an EPO tumor."
**P**heochromocytoma
**H**epatoma
**U**terine leiomyoma
**C**erebellar hemangioblastoma
**K**idney
151
What do kidney tumors often secrete?
EPO
| (due to VHL degradation)
152
Erythropoeitin, thrombopoeitin, and other hematopoeitic substances all bind receptors on what type of cell to induce differentiation down the various hematopoeitic lineages?
Hematopoeitic stem cells
153
What transcription factor is activated in the renal tubules to cause EPO release?
What stimulates this dimerization?
HF-1α dimerizes to HF-1β;
hypoxia
154
What molecular effect does hypoxia have on the renal tubules?
HF-1α dimerizes to HF-1β
(transcription factors)
--\>
increased EPO synthesis and secretion
155
What are HF-1α and HF-1β?
The renal transcription factors that increase EPO secretion in hypoxic conditions
156
What protein inhibits HF-1α during non-hypoxic times, inhibiting EPO synthesis?
Via what mechanism?
VHL
(von Hippel-Lindau);
increased ubiquination/degradation of HF-1α
157
What is VHL (von Hippel-Lindau) protein's function in the renal tubules?
To bind HF-1α and **prevent EPO synthesis**
158
What hypoxia-induced transcription factor causes increased EPO secretion from the renal tubular epithelium?
What protein inhibits this transcription factor through ubiquination in non-hypoxic conditions?
HF-1α;
VHL
159
Upon HF-1α mediated synthesis and release of EPO from the kidneys, what happens next?
EPO travels hematogenously to reach hematopoeitic stem cells in the bone marrow
--\>
it binds JAK2 receptors (dimerization)
--\>
JAK is phosphorlated
--\>
STAT is phosphorlyated
--\>
Erythrocyte differentiation is stimulated
160
HF-1α is responsible for EPO synthesis in hypoxic conditions.
It also increases synthesis of what other signaling molecule?
**VEGF**
| (vascular endothelial growth factor)
161
What is a SNP?
A single nucleotide polymorphism
| (allelic change)
162
JAK-activating mutations result in what type of disorder?
Myeloproliferative neoplastic disorders
163
What does the monoclonal antibody ending -'zumab' signify?
A humanized monoclonal antibody
164
What does the monoclonal antibody ending -'momab' signify?
A fully murine (mouse) monoclonal antibody
165
What does the monoclonal antibody ending -'ximab' signify?
A chimeric monoclonal antibody
166
What does the monoclonal antibody ending -'mumab' signify?
A fully human monoclonal antibody
167
Name the monoclonal antibody suffix for each of the following:
a **chimeric** monoclonal antibody
a **fully murine (mouse)** monoclonal antibody
a **fully human** monoclonal antibody
a **humanized** monoclonal antibody
- ximab
- momab
- mumab
- zumab
