Birds 1 Flashcards
(98 cards)
Lymphoid tissues what are the 2 types and things within
1) Primary
- thymus (T cells)
- Cloacal bursa (B cells)
2) Secondary
- Spleen
- Harderian glands
- caecal tonsils
- GALT - gastrointestinal associated lymphoid tissues
- MALT - mucosal associated lymphoid tissues
- BALT - bronchial associated lymphoid tissues
- CALT - conjunctival associated lymphoid tissues
○ No lymph nodes in birds except for water fowl (have structures that resemble lymph nodes)
Primary lymphoid tissues what are the 2 types and describe their structure and function
1) Thymus -> T cells
§ 7 lobes with lobules (cortical and medullary areas)
2) Cloacal bursa -> B cells
§ Starts growing when hatches, when gets to sexually maturity starts to shrink
□ Largest at 6-8 weeks of age
§ Lots of folds -> with follicles and specialised epithelial cells that present antigens leading to clonal expansion
Secondary lymphoid tissues what are the 3 main ones, location and function
1) Spleen - B and T cells present (not produced just move into)
§ Located underneath the proventriculus
§ In poultry round, other species can be elongated or triangular
2) Harderian glands
§ Underneath the eyeball (ventral aspect)
Lots of Plasma cells -> local antibody response for URT diseases
3) Caecal Tonsils
§ Not all birds have caecal tubes and therefore don’t have tonsil - chickens do
§ Both B and T cells
Vaccination programs what is important and the types based on
○ Timing is important and every bird needs to get the vaccine
○ Different programs depending on the type of production
§ Broilers -> only live average of 45 days, so don’t get diseases of older just younger birds
□ Only vaccinate against -> Infectious bronchitis virus vaccine
® Some use Marek’s disease, Infectious Laryngotracheitis virus and at outbreak Newcastle disease
§ Layer -> live a lot longer and egg production is important (not just clinical but also poor performance)
□ A whole range of vaccines used such as Infectious bronchitis
§ Breeders -> need maternal antibodies to be passed to progeny (antibodies last 3-6 weeks in chick)
□ FAV -> transmitted vertically and can kill progeny but doesn’t affect adults
Vaccination techniques list the main types
1) Mass vaccination
1. drinking water
2. spray vaccination
2) single vaccination
1. eye droplets
2. injections
Mass vaccination what are the 2 main techniques how works and advantages and disadvantages
1) Drinking water -> tricky because the vaccine can get inactivated within the water before get to the end of the shed
□ Generally cut off the water for 2-4 hours depending on season and then release when chickens are thirsty
□ Most farms have medication tanks and dye in the vaccine helps knowing where vaccine is
® Scoring system, scores the birds based on dye on the tongue of the bird
□ Disadvantages -> minerals in the water can inactivate the virus - ILT, Newcastle disease
® Need to add products to the water before give vaccine
2) Spray vaccination -> different size of the droplets (larger droplets for upper respiratory system such as IB)
□ Vaccinated in crates or cabinets - easier when they are young
□ Can do in older birds but need larger equipment
Single vaccination what are the 2 mechanisms and how they work
1) Eye droplet -> individually administer vaccine -> most expensive vaccine due to cost of labour
□ Stressful for the birds so not ideal but generally done at once with multiple procedures to save on labour
□ Again dye the vaccine to check the eyes of the birds
2) Injections
□ Subcutaneous injection or muscle
□ Fowl pox injected via two way needle into the wing
□ New technology can vaccinate the embryos before they hatch - embrex
® Earlier immunity, uniform and fast delivery, reduced stress and labour cost
What are the 2 main types of diseases of the immune system and diseases within
- Neoplastic ○ Marek’s Disease ○ Lymphoid Leucosis - Immunosuppressive ○ Infectious bursal disease ○ Chicken infectious anaemia ○ Others
Marek’s disease what birds affected, clinical signs and aetiology
- Only chickens are affected, fairly rare due to vaccine
- Insignificant in terms of the poultry industry
- Sulkies particularly infected
Signs - Usually 3-4 months (point of lay)
- Paresis/paralysis - unable to walk or lameness
○ wing, leg, neck
○ respiratory, GIT - if these nerves are affected - Non-specific signs
Aetiology - Herpesvirus
- Three serotypes -> evolving and increasing in virulence over the years
○ serotype I -> pathogenic, a spectrum of virulence
○ serotypes II & III (HVT) -> ubiquitous but avirulent
List 6 diagnostic techniques for mareks disease
- Age - 3-4 months of age
- Clinical signs (paralysis)
- Gross lesions (nerves, skin & eye)
○ Swelling and discolouration of the nerve, sciatic nerve is a good one, generally only one side of the neuron - Histopathology
○ Made of lymphocytes (T cells) -> mature and immature - polymorphic -> lymphomatous infiltrations of nerves/skin/organs
§ Lymphoid leucosis -> stain with B cell markers not T cell so how to tell the difference - Immunohistochemistry (T cells)
- PCR
What are the 4 steps in the pathogenesis of mareks disease
- Infection by inhalation
- Acute phase - growth of virus in lymphoid tissue (bursa, thymus), degenerative lesions -> premature bursal/thymic atrophy
- Latent phase, virus carried in lymphocytes, inflammatory lesions in nerves
- Proliferation of lymphoid cells, lymphoma formation, lymphomatous infiltration of nerves and viscera. Malignantly transformed cells are T-cells through both T and B cells participate in lesions
What are the 5 main factors affecting development of the disease
- Virus pathogen virulence and pathogenicity
- Environment, degree of exposure
- Host - age at first exposure, genetic resistance/susceptibility
- Sex - females are more susceptible than males
- Immune status - vaccination protects against development but not infection
What is transmission and control of mareks disease
MDV Transmission
1. Very early in life - first day
2. Horizontal
○ Complete virus produced only in feather follicle epithelium so excreted in feather dander, keratin
○ Infectious within the environment for a long time - can never eradicate it
3. No vertical
Control
- No treatment!
- Hygiene, age separation, pest & insect control, genetic selection do not provide total protection
“Vaccination” -> injected to back of neck at 1 day old, or the embryo injection machine
Lymphoid leucosis what age, clinical signs, transmission and mechanism
- Naturally occurs in chickens
- Usually > 5 m
- Clinical Signs - non-specific
○ Reduced weight, weakness & anorexia
○ Egg parameters, hatchability & fertility
○ Non-specific mortality
Transmission - Virus generally comes from the parents
○ Chicks hatch with viraemia and shed from all systems (respiratory, gastrointestinal, reproductive)
Mechanism - Affect B cells within the cloacal bursa and B cells become tumour and metastasise to other organs
Lymphoid leucosis describe 6 ways to diagnose lymphoid leucosis
- Age -> < 5months
- Lesions
○ Cloacal bursa ALWAYS affected
○ B cells involved - Histopathology
○ Doesn’t generally affect skeletal muscle, eyes or nerves unlike Marek’s disease
○ Lymphoblast’s not lymphocytes - Immunohistochemistry
○ B cell marker staining works - different to Marek’s disease - PCR
○ Need to determine which PCR -> such as Lymphoid leucosis PCR - Serology
○ Detect antibodies however with immunotolerance some birds infected don’t produce antibodies
Lymphoid leucosis pathogenesis and cause
Pathogenesis
1. Infection in ovo leads to disease about 4-5months
2. Neoplastic transformation occurs initially in the bursa of fabricius
3. Nodular tumours are evident from about 14 weeks and metastasize from bursa to other organs
LL Virus
- A member of the avian leucosis/sarcoma viruses (ALV)
- Other members cause:
○ myelocytomatosis
○ Myeloblastoma & erythroblastoma
Haemangioma, nephroblastoma, fibrosarcoma, osteopetrosis, etc.
Lymphoid leucosis what are the two types of transmission and control
Exogenous and Endogenous Viruses Transmission
Exogenous
1. Transmitted through the egg via parents -> progeny
2. Horizontal via infectious virus
Endogenous
1. Genetic -> viral DNA integrated in gamete DNA -> can start to produce virus
Control
- No vaccine
- Eradication via selection for genetic resistance and detection of carrier hens
○ Carrier hens -> detected via vaginal swabs, carrier birds culled from breeder flock
Infectious bursal disease (IBD) what birds does it affect, virus character and the 3 diseases forms
- Only in chickens but is very significant worldwide
- Very hardy virus, cooking chicken the virus is still viable
- Disease forms
a. Immunosuppression, <3 wo
§ Succumb to other diseases
b. Clinical disease, 3-6 wo
c. Severe form, any age
Infectious bursal disease (IBD) what are the 3 main mechanism forms and the weeks involved
1) inapparent form (<3 weeks)
2) acute form (3-6 weeks)
3) very severe form
Inapparent form for infectious bursal disease what are the clinical signs and lesions
○ Subtle clinical signs
§ Mortality apparently due to other diseases
□ Trying to control multiple bacterial infections and cannot control - THINK THIS
§ Poor vaccination response due to the virus affecting the B lymphocytes
○ Lesions -> cloacal bursa may be very small for its age
Acute form of infectious bursal disease clinical signs, lesions and onset
- Clinical signs ○ "sick chicken" -> chicken hunched over, fluffed up, low acitivty ○ Polyuria causing running droppings - Lesions ○ Bursa is swollen ○ May see haemorrhage in skeletal muscle - Immunosuppression - Rapid onset (IP 2-3d) and course Mortality up and then back to normal
Very severe form of infectious bursal disease where presnt, what caused by, clinical signs, affected tissues and age
- “EXOTIC TO AUSTRALIA”
- Caused by vvIBDV (very virulent)
- Clinical signs & lesions as acute form
- Also affects other lymphoid tissues eg. thymus
Affects any age eg. >6 w
Infectious bursal disease what are the 4 main diagnostic techniques
- Rapid course
- Bursal lesions* - swelling, oedema, haemorrhage, atrophy
- Detection of Virus
○ RT-PCR -> RNA viruses
○ AGP - Serology (ELISA) - mainly for monitoring
Infectious bursal disease what are some control methods
- Hygiene does NOT eliminate the virus but can reduce level of infection - all in - all out
- Biosecurity
○ Source of feed - not contaminated
○ Insect control - beetles can carry the virus - Passive antibody* - MATERNAL ANTIBODIES ARE THE BEST
○ How done in Australia
○ Different strains in different areas so need to strain type to ensure vaccine has local strain
Vaccination of Non-breeder Flocks - NOT IN AUS - If the risk of vIBDV is high
- Passive antibodies interference
○ “antibody profiling” - 1-3 live, moderately virulent vaccines